Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K0H81_RS14400 Genome accession   NZ_CP080417
Coordinates   3316907..3318019 (-) Length   370 a.a.
NCBI ID   WP_011864943.1    Uniprot ID   A3QC12
Organism   Shewanella halotolerans strain FJAT-53555     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3311907..3323019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H81_RS14375 (K0H81_14375) - 3311984..3312424 (-) 441 WP_220058765.1 Imm44 family immunity protein -
  K0H81_RS14380 (K0H81_14380) - 3312527..3313000 (-) 474 WP_220058766.1 hypothetical protein -
  K0H81_RS14385 (K0H81_14385) - 3313404..3314606 (-) 1203 WP_220058389.1 IS4 family transposase -
  K0H81_RS14390 (K0H81_14390) - 3314650..3315057 (-) 408 WP_220058767.1 hypothetical protein -
  K0H81_RS14395 (K0H81_14395) hemH 3315713..3316726 (-) 1014 WP_220058768.1 ferrochelatase -
  K0H81_RS14400 (K0H81_14400) pilU 3316907..3318019 (-) 1113 WP_011864943.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0H81_RS14405 (K0H81_14405) pilT 3318032..3319069 (-) 1038 WP_011864942.1 type IV pilus twitching motility protein PilT Machinery gene
  K0H81_RS14410 (K0H81_14410) - 3319105..3319806 (+) 702 WP_220058769.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0H81_RS14415 (K0H81_14415) proC 3319891..3320709 (+) 819 WP_220058770.1 pyrroline-5-carboxylate reductase -
  K0H81_RS14420 (K0H81_14420) - 3320769..3321317 (+) 549 WP_144201230.1 YggT family protein -
  K0H81_RS14425 (K0H81_14425) yggU 3321318..3321608 (+) 291 WP_220044600.1 DUF167 family protein YggU -
  K0H81_RS14430 (K0H81_14430) - 3321695..3322129 (+) 435 WP_011864937.1 DUF4426 domain-containing protein -
  K0H81_RS14435 (K0H81_14435) rdgB 3322271..3322870 (+) 600 WP_220058771.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41143.26 Da        Isoelectric Point: 6.3733

>NTDB_id=592743 K0H81_RS14400 WP_011864943.1 3316907..3318019(-) (pilU) [Shewanella halotolerans strain FJAT-53555]
MEVRPFLKVMVERKASDLFITAGFPPSAKVDGEVRPLAENPFTPAQSLEFVEALMTEAQRKEFHETRECNFAYGEKGLGR
FRVSAFWQRESPGCVMRRIETKIPLVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNSHARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEVLINTPRVASIIQKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVEGEISYSDALHHADSPNDLRLMIKLQSSESASSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=592743 K0H81_RS14400 WP_011864943.1 3316907..3318019(-) (pilU) [Shewanella halotolerans strain FJAT-53555]
ATGGAAGTTCGTCCATTTTTAAAGGTGATGGTAGAGCGCAAGGCGTCGGATCTCTTCATCACCGCCGGCTTTCCCCCCAG
CGCTAAGGTTGATGGCGAGGTGCGCCCCTTAGCCGAGAACCCTTTTACCCCGGCTCAGTCGCTGGAGTTTGTCGAGGCCC
TGATGACAGAGGCCCAGCGTAAAGAGTTTCACGAGACCCGCGAATGTAACTTCGCCTATGGCGAGAAGGGCCTGGGACGT
TTTCGTGTCAGCGCCTTCTGGCAGCGCGAGTCACCGGGCTGCGTGATGCGTCGTATCGAGACCAAGATCCCGCTGGTGGA
AGATCTTAAGCTGCCACCAATCCTGAAAGACCTGGTAATGAGTAAGCGTGGCCTTATCATCATGGTGGGTGGTACGGGTA
CGGGTAAGTCGACCTCGCTGGCGGCGCTGGTGGGTTACCGCAACTCCCACGCCCGCGGCCATATCCTCACCATCGAAGAT
CCGGTGGAATTTGTCCACGACCATCGCAAGAGCATCATCACCCAGCGTGAGGTGGGTATAGATACCGACTCTTTCGATGC
CGCGCTCAAGAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGTGAGATCCGTACCCAGGAGACCATGGAGTTTG
CTCTGTCGTTCGCCGAAACCGGTCACCTCTGTATGGCTACCCTGCACGCCAACAACGCTAACCAGGCGCTGGACCGCATC
ATGCACTTGGTGCCCGAGAGTAAGCACCAGCAGCTGCTGTTTGACTTGTCGCTGAACCTTCGCGGCATCGTGGCGCAGCA
ACTTGTGCCTAAGGCAGATGGCACGGGTCGCCGAGCGGCCATCGAGGTGTTGATCAACACCCCAAGGGTTGCCAGCATTA
TCCAGAAGAACGAGCTGCATCTGCTTAAGGAAACCATGGCCAAATCCAACGAGCAGGGGATGCAGACCTTCGACCAGGCG
CTGCTTAAGCTCTATGTGGAAGGTGAGATCAGCTACTCGGATGCGCTGCACCACGCCGACTCGCCAAACGATCTGCGCTT
GATGATCAAGCTGCAAAGCTCAGAATCGGCAAGCTCAGGTTTCATGGAAGGTGTGACGCTGGATCTCGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3QC12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Acinetobacter baylyi ADP1

59.889

97.027

0.581
  pilU Vibrio cholerae strain A1552

58.108

100

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.444

97.297

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.189

0.373
  pilT Vibrio cholerae strain A1552

41.818

89.189

0.373
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362