Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K0H79_RS12935 Genome accession   NZ_CP080415
Coordinates   2968765..2969802 (-) Length   345 a.a.
NCBI ID   WP_220065175.1    Uniprot ID   -
Organism   Shewanella sp. FJAT-52076     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2963765..2974802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H79_RS12900 (K0H79_12900) - 2964317..2964877 (-) 561 WP_220065170.1 DUF4136 domain-containing protein -
  K0H79_RS12905 (K0H79_12905) - 2965068..2965397 (+) 330 WP_220076084.1 hypothetical protein -
  K0H79_RS12910 (K0H79_12910) yciH 2965511..2965840 (+) 330 WP_220065172.1 stress response translation initiation inhibitor YciH -
  K0H79_RS12915 (K0H79_12915) - 2965849..2966412 (+) 564 WP_220078160.1 YqgE/AlgH family protein -
  K0H79_RS12920 (K0H79_12920) ruvX 2966412..2966831 (+) 420 WP_220065173.1 Holliday junction resolvase RuvX -
  K0H79_RS12925 (K0H79_12925) - 2966899..2967441 (-) 543 WP_220078161.1 glutathione peroxidase -
  K0H79_RS12930 (K0H79_12930) pilU 2967643..2968755 (-) 1113 WP_220065174.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0H79_RS12935 (K0H79_12935) pilT 2968765..2969802 (-) 1038 WP_220065175.1 type IV pilus twitching motility protein PilT Machinery gene
  K0H79_RS12940 (K0H79_12940) - 2969838..2970545 (+) 708 WP_220076085.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0H79_RS12945 (K0H79_12945) proC 2970637..2971452 (+) 816 WP_220076086.1 pyrroline-5-carboxylate reductase -
  K0H79_RS12950 (K0H79_12950) - 2971498..2972046 (+) 549 WP_220065178.1 YggT family protein -
  K0H79_RS12955 (K0H79_12955) yggU 2972046..2972333 (+) 288 WP_220076087.1 DUF167 family protein YggU -
  K0H79_RS12960 (K0H79_12960) - 2972416..2972850 (+) 435 WP_220076088.1 DUF4426 domain-containing protein -
  K0H79_RS12965 (K0H79_12965) rdgB 2972982..2973578 (+) 597 WP_220076089.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K0H79_RS12970 (K0H79_12970) hemW 2973588..2974727 (+) 1140 WP_220076090.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38263.93 Da        Isoelectric Point: 6.9095

>NTDB_id=592695 K0H79_RS12935 WP_220065175.1 2968765..2969802(-) (pilT) [Shewanella sp. FJAT-52076]
MEITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHAGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEQLGAPDIFKKIASFPRGLVLVTGPTGSGKSTTLAGMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=592695 K0H79_RS12935 WP_220065175.1 2968765..2969802(-) (pilT) [Shewanella sp. FJAT-52076]
ATGGAAATCACAGAGTTACTGGCGTTTAGTGTAAAGCACAAGGCCTCGGATCTACACCTCTCTGCCGGTGTTCCCCCCAT
GATCCGGGTGGACGGTGAAGTGCGTAAAATCAATCTGCCTGCACTCGATCATGCCGGCGTACACTCCTTGGTGTACGACA
TTATGAATGACAAGCAGCGCAAAGACTATGAAGAGCACCTGGAAATCGACTTTTCCTTTGAAGTGCCCAATCTTGCCCGT
TTCCGTGTAAACGCCTTTAACCAATCCCGCGGCGCGGCTGCGGTGTTTCGTACCATTCCCAGCGAAATTCTGTCGCTGGA
GCAACTGGGCGCCCCGGACATCTTCAAAAAAATCGCCAGTTTCCCCCGTGGTCTGGTGCTGGTGACCGGCCCAACCGGCT
CCGGTAAGAGTACCACCCTGGCAGGCATGATTGACTACGTGAACGAAAACCGTCACGACCACATTCTGACCATCGAAGAC
CCTATCGAATTCGTGCACCAAAATAAGCAGTGTCTTATCAACCAGCGGGAAGTGCATCGCCATACCCACAGCTTTAACGC
GGCCCTTCGCAGCGCGCTGCGTGAAGACCCCGACGTGATTCTGGTGGGTGAGATGCGTGACCTTGAAACCATACGTCTGG
CGATGACAGCGGCAGAAACCGGTCACCTGGTTTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGTGTG
GTCGACGTATTCCCGGCCGGTGAAAAAGACATGGTGCGTACCATGTTGTCAGAATCCTTGCAGGCGGTAATTTCCCAGAC
GCTGATCAAGAAGATTGGCGGCGGTCGGGTAGCAGCCCACGAAATTATGATGGGTACCCCTGCGATTCGTAACCTTATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCCATTCAAACCGGTATGGCCCATGGCATGCAAACCCTCGAGCAGTGTCTG
CAGAATCTGGTGAACCGTGGCCTCATCACCCGTGAAGACGCCATGGCCAAGAGTTCCAACAAACAGGCAAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.551

99.42

0.771
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

76.812

100

0.768
  pilT Legionella pneumophila strain Lp02

75.66

98.841

0.748
  pilT Legionella pneumophila strain ERS1305867

75.66

98.841

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.857

99.42

0.426
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

40.356

97.681

0.394