Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K0H79_RS12930 Genome accession   NZ_CP080415
Coordinates   2967643..2968755 (-) Length   370 a.a.
NCBI ID   WP_220065174.1    Uniprot ID   -
Organism   Shewanella sp. FJAT-52076     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2962643..2973755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H79_RS12890 (K0H79_12890) - 2962827..2963276 (-) 450 WP_220076082.1 PACE efflux transporter -
  K0H79_RS12895 (K0H79_12895) - 2963364..2964245 (+) 882 WP_220076083.1 LysR family transcriptional regulator -
  K0H79_RS12900 (K0H79_12900) - 2964317..2964877 (-) 561 WP_220065170.1 DUF4136 domain-containing protein -
  K0H79_RS12905 (K0H79_12905) - 2965068..2965397 (+) 330 WP_220076084.1 hypothetical protein -
  K0H79_RS12910 (K0H79_12910) yciH 2965511..2965840 (+) 330 WP_220065172.1 stress response translation initiation inhibitor YciH -
  K0H79_RS12915 (K0H79_12915) - 2965849..2966412 (+) 564 WP_220078160.1 YqgE/AlgH family protein -
  K0H79_RS12920 (K0H79_12920) ruvX 2966412..2966831 (+) 420 WP_220065173.1 Holliday junction resolvase RuvX -
  K0H79_RS12925 (K0H79_12925) - 2966899..2967441 (-) 543 WP_220078161.1 glutathione peroxidase -
  K0H79_RS12930 (K0H79_12930) pilU 2967643..2968755 (-) 1113 WP_220065174.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0H79_RS12935 (K0H79_12935) pilT 2968765..2969802 (-) 1038 WP_220065175.1 type IV pilus twitching motility protein PilT Machinery gene
  K0H79_RS12940 (K0H79_12940) - 2969838..2970545 (+) 708 WP_220076085.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0H79_RS12945 (K0H79_12945) proC 2970637..2971452 (+) 816 WP_220076086.1 pyrroline-5-carboxylate reductase -
  K0H79_RS12950 (K0H79_12950) - 2971498..2972046 (+) 549 WP_220065178.1 YggT family protein -
  K0H79_RS12955 (K0H79_12955) yggU 2972046..2972333 (+) 288 WP_220076087.1 DUF167 family protein YggU -
  K0H79_RS12960 (K0H79_12960) - 2972416..2972850 (+) 435 WP_220076088.1 DUF4426 domain-containing protein -
  K0H79_RS12965 (K0H79_12965) rdgB 2972982..2973578 (+) 597 WP_220076089.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41117.13 Da        Isoelectric Point: 6.0929

>NTDB_id=592694 K0H79_RS12930 WP_220065174.1 2967643..2968755(-) (pilU) [Shewanella sp. FJAT-52076]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAENAFSPAQSLDFVESLMSDAQKKEFHESRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEMEDLKLPPVLKDLVMSKRGLIIMVGGTGTGKSTSLAAMVGYRNAHERGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRVSSIIQKNELHLLKDTMAKSNEQGMQTFDQA
LFKLYQEGEITYADALHHADSPNDLRLMIKLAGSESTGSGFLEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=592694 K0H79_RS12930 WP_220065174.1 2967643..2968755(-) (pilU) [Shewanella sp. FJAT-52076]
ATGGATGTACGTCCCTTTCTGAAGGTGATGGTCGAGCGTAAAGCCTCGGATTTGTTCATCACCGCAGGTTTTCCTCCCAG
CGCCAAAATCGATGGTGAGTTACGGCCTCTGGCGGAAAATGCCTTCAGCCCCGCCCAGTCACTGGACTTTGTTGAGTCCC
TGATGTCGGATGCGCAAAAGAAAGAGTTTCACGAGAGCCGTGAGTGTAACTTCGCCTTTGCCGCCAAAGACCTGGGACGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCTCCCGGCTGCGTAATGCGCCGTATCGAGACCAAAATCCCTGAGATGGA
AGATCTTAAACTGCCTCCTGTGCTTAAAGATCTGGTGATGAGTAAGCGTGGTCTTATCATCATGGTGGGTGGTACAGGTA
CCGGTAAGTCGACGTCGCTGGCGGCCATGGTGGGGTATCGTAATGCCCATGAGCGAGGTCATATCCTGACCATCGAAGAC
CCGGTGGAATTTGTGCACGACCACCGCAAGAGCATCATCACTCAGCGGGAAGTGGGTATCGATACCGACTCCTTCGACGC
GGCGCTGAAAAGCTCGCTGCGTCAGGCCCCGGACGTGATCCTTATCGGTGAAATTCGAAGCCAGGAGACCATGGAGTTTG
CCCTGTCATTCGCCGAAACCGGTCACCTCTGTATGGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATGCACCTGGTGCCGGAGAGCAAGCACCAGCAGCTGTTGTTTGACCTGTCACTCAACCTGCGTGGCATTGTGGCGCAGCA
ACTGGTGCCCAAGGCCGATGGCACTGGGCGCCGTGCGGCCATTGAAATTCTGATTAACACGCCCCGTGTGTCGAGCATTA
TTCAGAAAAATGAGCTGCATTTGCTTAAAGACACCATGGCCAAGTCCAATGAGCAGGGAATGCAGACCTTTGACCAGGCG
CTGTTCAAGCTCTATCAGGAAGGTGAAATCACCTACGCCGATGCCTTGCACCATGCCGACTCGCCAAACGATCTGCGTTT
GATGATCAAGCTCGCAGGCTCCGAAAGCACGGGTTCCGGCTTCCTGGAAGGCGTGACTCTGGACATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.29

100

0.616
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

60.34

95.405

0.576
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.667

97.297

0.405
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.03

89.189

0.384
  pilT Vibrio cholerae strain A1552

43.03

89.189

0.384
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Acinetobacter nosocomialis M2

40.058

92.432

0.37
  pilT Acinetobacter baumannii D1279779

40.058

92.432

0.37
  pilT Acinetobacter baumannii strain A118

40.058

92.432

0.37
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Pseudomonas stutzeri DSM 10701

39.13

93.243

0.365
  pilT Pseudomonas aeruginosa PAK

39.13

93.243

0.365