Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K0H59_RS06455 Genome accession   NZ_CP080411
Coordinates   1422861..1423898 (+) Length   345 a.a.
NCBI ID   WP_011716282.1    Uniprot ID   A0A2M7HST3
Organism   Shewanella sp. FJAT-51649     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1417861..1428898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H59_RS06425 (K0H59_06425) rdgB 1418873..1419490 (-) 618 WP_220055003.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K0H59_RS06430 (K0H59_06430) - 1419651..1420085 (-) 435 WP_011621977.1 DUF4426 domain-containing protein -
  K0H59_RS06435 (K0H59_06435) yggU 1420154..1420444 (-) 291 WP_011621978.1 DUF167 family protein YggU -
  K0H59_RS06440 (K0H59_06440) - 1420444..1420992 (-) 549 WP_220055004.1 YggT family protein -
  K0H59_RS06445 (K0H59_06445) proC 1421069..1421887 (-) 819 WP_220055005.1 pyrroline-5-carboxylate reductase -
  K0H59_RS06450 (K0H59_06450) - 1422109..1422825 (-) 717 WP_220055006.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0H59_RS06455 (K0H59_06455) pilT 1422861..1423898 (+) 1038 WP_011716282.1 type IV pilus twitching motility protein PilT Machinery gene
  K0H59_RS06460 (K0H59_06460) pilU 1423908..1425020 (+) 1113 WP_011716283.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0H59_RS06465 (K0H59_06465) - 1425197..1425730 (+) 534 WP_220055566.1 glutathione peroxidase -
  K0H59_RS06470 (K0H59_06470) hemH 1425800..1426783 (+) 984 WP_220055007.1 ferrochelatase -
  K0H59_RS06475 (K0H59_06475) ruvX 1426844..1427269 (-) 426 WP_220055008.1 Holliday junction resolvase RuvX -
  K0H59_RS06480 (K0H59_06480) - 1427300..1427863 (-) 564 WP_011621987.1 YqgE/AlgH family protein -
  K0H59_RS06485 (K0H59_06485) yciH 1427932..1428261 (-) 330 WP_220055009.1 stress response translation initiation inhibitor YciH -
  K0H59_RS06490 (K0H59_06490) - 1428411..1428725 (-) 315 WP_011716287.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38356.02 Da        Isoelectric Point: 6.9097

>NTDB_id=592596 K0H59_RS06455 WP_011716282.1 1422861..1423898(+) (pilT) [Shewanella sp. FJAT-51649]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=592596 K0H59_RS06455 WP_011716282.1 1422861..1423898(+) (pilT) [Shewanella sp. FJAT-51649]
ATGGAAATCACTGAGTTATTAGCCTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATTAACCTGCCCGCGCTCGATCATCAGGGTGTACATAGCCTTGTGTACGACA
TTATGAATGATAAACAGCGTAAGGACTTTGAAGAGCATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTAAACGCCTTTAACCAATCCCGCGGCGCGGCGGCGGTATTTCGTACCATTCCAAGCGAAATTTTGTCGCTCGA
GCAGTTAGGTGCGCCAGAGATTTTTAAAAAAATTTCCAGCTTTCCCCGCGGCTTAGTGCTTGTTACTGGGCCTACAGGTT
CGGGTAAGAGTACCACACTTGCGGCCATGGTGGATTACATCAATGAGAACCGCCACGACCATATCTTAACCATTGAAGAT
CCTATTGAATTCGTTCACCAGAATAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGCCATACCCACAGCTTTAACGC
AGCGCTACGTAGCGCACTGCGTGAAGACCCTGACGTTATTCTCGTCGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CGATGACGGCGGCCGAAACGGGTCACTTAGTTTTTGGTACCTTGCACACCACCTCGGCGGCTAAGACCATCGACCGTGTG
GTTGACGTTTTCCCAGCTGGAGAAAAGGACATGGTGCGTACCATGTTGTCTGAATCATTACAGGCGGTTATTTCGCAAAC
CCTGATTAAGAAAATTGGTGGCGGCCGTGTGGCTGCCCACGAAATCATGATGGGTACGCCCGCTATCCGTAACCTTATCC
GTGAAGATAAAGTAGCGCAGATGTACTCAGCCATTCAAACGGGGATGGCCCATGGCATGCAAACGCTCGAACAGTGTCTG
CAAAACTTAGTCAACCGTGGCCTCATCACCCGTGAGGATGCCATGTCGAAGAGTTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2M7HST3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.592

99.42

0.791
  pilT Acinetobacter baumannii strain A118

79.592

99.42

0.791
  pilT Acinetobacter nosocomialis M2

79.592

99.42

0.791
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Acinetobacter baylyi ADP1

80.547

95.362

0.768
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

41.369

97.391

0.403
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388