Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K0H59_RS06460 Genome accession   NZ_CP080411
Coordinates   1423908..1425020 (+) Length   370 a.a.
NCBI ID   WP_011716283.1    Uniprot ID   A0A220UQ50
Organism   Shewanella sp. FJAT-51649     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1418908..1430020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H59_RS06430 (K0H59_06430) - 1419651..1420085 (-) 435 WP_011621977.1 DUF4426 domain-containing protein -
  K0H59_RS06435 (K0H59_06435) yggU 1420154..1420444 (-) 291 WP_011621978.1 DUF167 family protein YggU -
  K0H59_RS06440 (K0H59_06440) - 1420444..1420992 (-) 549 WP_220055004.1 YggT family protein -
  K0H59_RS06445 (K0H59_06445) proC 1421069..1421887 (-) 819 WP_220055005.1 pyrroline-5-carboxylate reductase -
  K0H59_RS06450 (K0H59_06450) - 1422109..1422825 (-) 717 WP_220055006.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0H59_RS06455 (K0H59_06455) pilT 1422861..1423898 (+) 1038 WP_011716282.1 type IV pilus twitching motility protein PilT Machinery gene
  K0H59_RS06460 (K0H59_06460) pilU 1423908..1425020 (+) 1113 WP_011716283.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0H59_RS06465 (K0H59_06465) - 1425197..1425730 (+) 534 WP_220055566.1 glutathione peroxidase -
  K0H59_RS06470 (K0H59_06470) hemH 1425800..1426783 (+) 984 WP_220055007.1 ferrochelatase -
  K0H59_RS06475 (K0H59_06475) ruvX 1426844..1427269 (-) 426 WP_220055008.1 Holliday junction resolvase RuvX -
  K0H59_RS06480 (K0H59_06480) - 1427300..1427863 (-) 564 WP_011621987.1 YqgE/AlgH family protein -
  K0H59_RS06485 (K0H59_06485) yciH 1427932..1428261 (-) 330 WP_220055009.1 stress response translation initiation inhibitor YciH -
  K0H59_RS06490 (K0H59_06490) - 1428411..1428725 (-) 315 WP_011716287.1 hypothetical protein -
  K0H59_RS06495 (K0H59_06495) - 1428905..1429465 (+) 561 WP_011621990.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41173.39 Da        Isoelectric Point: 6.3725

>NTDB_id=592597 K0H59_RS06460 WP_011716283.1 1423908..1425020(+) (pilU) [Shewanella sp. FJAT-51649]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESVMTEAQKKEFHSTRECNFAFAVKDLGR
FRVSAFWQRESPGCVMRRIETKIPEVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLQLYIDGEISYADALHHADSPNDLRLMIKLQNKEPTSSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=592597 K0H59_RS06460 WP_011716283.1 1423908..1425020(+) (pilU) [Shewanella sp. FJAT-51649]
ATGGATGTCCGTCCGTTTTTAAAAGTCATGGTGGAGCGTAAAGCCTCGGACTTGTTTATTACCGCAGGTTTTCCGCCGAG
CGCCAAAATCGATGGTGAGTTACGCCCATTGGCCGAGAGTGCCTTCACGCCCGCGCAGTCTTTGGATTTTGTTGAATCCG
TGATGACTGAGGCGCAAAAGAAGGAGTTTCACAGCACTCGCGAGTGTAACTTCGCTTTTGCGGTGAAGGATTTAGGGCGT
TTTCGCGTTAGCGCATTCTGGCAGCGTGAATCTCCGGGATGCGTGATGCGCCGGATTGAGACCAAGATCCCAGAGGTGGA
AGACTTAAAACTGCCACCGATTTTAAAAGATCTGGTGATGAGTAAACGGGGTCTTATCATCATGGTCGGGGGTACGGGAA
CCGGTAAGTCGACCTCTTTGGCGGCGTTAGTGGGTTATCGTAATGCCCATGCCCGTGGTCATATCCTTACCATCGAAGAC
CCAGTGGAATTTGTGCACGATCATCGCAAAAGTATCATCACTCAACGTGAAGTGGGTATAGATACAGAATCCTTTGATGC
GGCGCTGAAGAGTTCGCTGCGTCAAGCGCCCGATGTGATTTTGATTGGTGAAATTCGTACCCAAGAAACCATGGAATTTG
CCCTTTCCTTCGCCGAAACGGGTCACCTCTGTATGGCGACACTGCACGCGAACAACGCTAACCAAGCGTTAGACCGGATC
ATGCACTTAGTACCTGAGAGTAAACATCAACAGTTGCTATTCGACTTGTCACTCAACTTGCGCGGCATTGTGGCGCAGCA
ACTAGTGCCTAAGGTCGATGGCACAGGACGACGGGCGGCGATTGAAGTCTTGATCAATACGCCACGTGTCGCGAGCTTGA
TTGCTAAAAACGAGCTGCATTTGCTTAAAGAAACCATGGCTAAATCGAATGAGCAAGGCATGCAGACCTTCGACCAAGCC
TTGTTGCAACTCTATATCGATGGGGAAATCAGCTATGCCGATGCGCTTCACCATGCTGACTCGCCAAACGACTTGCGTTT
AATGATCAAACTGCAAAATAAAGAGCCTACCAGCTCAAGCTTTATGGAAGGCGTGACCTTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A220UQ50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Vibrio cholerae strain A1552

59.189

100

0.592
  pilU Acinetobacter baylyi ADP1

60.511

95.135

0.576
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.333

89.189

0.386
  pilT Vibrio cholerae strain A1552

43.333

89.189

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Acinetobacter nosocomialis M2

39.766

92.432

0.368
  pilT Acinetobacter baumannii D1279779

39.766

92.432

0.368
  pilT Acinetobacter baumannii strain A118

39.766

92.432

0.368
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Legionella pneumophila strain Lp02

41.358

87.568

0.362
  pilT Legionella pneumophila strain ERS1305867

41.358

87.568

0.362