Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KY496_RS23090 Genome accession   NZ_CP080379
Coordinates   5225284..5226327 (-) Length   347 a.a.
NCBI ID   WP_005668052.1    Uniprot ID   A0A1S2NFS0
Organism   Massilia sp. NP310     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5220284..5231327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KY496_RS23080 (KY496_23080) - 5221773..5223941 (+) 2169 WP_005668049.1 TonB-dependent siderophore receptor -
  KY496_RS23085 (KY496_23085) pilU 5224136..5225272 (-) 1137 WP_005668051.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KY496_RS23090 (KY496_23090) pilT 5225284..5226327 (-) 1044 WP_005668052.1 type IV pilus twitching motility protein PilT Machinery gene
  KY496_RS23095 (KY496_23095) - 5226402..5227103 (+) 702 WP_040776914.1 YggS family pyridoxal phosphate-dependent enzyme -
  KY496_RS23100 (KY496_23100) proC 5227115..5227927 (+) 813 WP_005668054.1 pyrroline-5-carboxylate reductase -
  KY496_RS23105 (KY496_23105) - 5228427..5228894 (-) 468 WP_040776918.1 hypothetical protein -
  KY496_RS23110 (KY496_23110) - 5229335..5230369 (-) 1035 WP_219862625.1 DUF4238 domain-containing protein -
  KY496_RS23115 (KY496_23115) - 5230896..5231240 (-) 345 WP_005668055.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37999.71 Da        Isoelectric Point: 6.7269

>NTDB_id=592436 KY496_RS23090 WP_005668052.1 5225284..5226327(-) (pilT) [Massilia sp. NP310]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKQYEEVLECDFSFAIPGLAR
FRVNAFNQERGASAVLRTIPSKILTLEELNAPKIFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHILTIED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDAILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIREAKIAQMYSAIQTGSNAGMQTLDQN
LTELVRRNVISTGAARAAAKIPENFLG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=592436 KY496_RS23090 WP_005668052.1 5225284..5226327(-) (pilT) [Massilia sp. NP310]
ATGGACATTTCGGAATTACTCGCCTTCTCGGTCAAGAACAAGGCTTCAGACTTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATTAACCTGCCGCCGCTCGAGCACAAGGACGTGCACGGCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGCAGTACGAAGAAGTGCTCGAGTGCGACTTCTCGTTCGCGATCCCTGGTCTTGCGCGC
TTCCGCGTCAACGCCTTCAACCAGGAGCGCGGCGCGTCGGCCGTGCTGCGCACGATTCCGTCCAAGATCCTGACCCTCGA
AGAGCTGAACGCCCCGAAGATCTTCGCCGACCTGGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CTGGTAAATCGACCACGCTGGCGGCGATGGTGAACCACCTGAACGAGAACGAGTACGGCCACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGCACACGCTGTCGTTCAACAA
CGCGCTGCGTTCGGCGCTGCGCGAAGACCCGGACGCGATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGTCGGCGGCCGAGACGGGTCACTTGGTGTTCGGCACCCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGAAGAGAAGGAGATGGTGCGCGCGATGCTGTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGCTCAAGACCAAGGACGGCTCGGGCCGCGTGGCGGCGCACGAAATCATGATCGGCACCCCGGCGATCCGCAACCTGA
TCCGCGAAGCCAAGATCGCCCAGATGTACTCTGCCATCCAGACCGGCAGCAATGCCGGCATGCAGACGCTGGACCAGAAC
CTGACGGAGCTGGTGCGCCGTAACGTGATCTCGACCGGCGCCGCGCGCGCCGCGGCCAAGATCCCGGAAAACTTCCTCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2NFS0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.324

99.712

0.781
  pilT Acinetobacter baumannii D1279779

78.324

99.712

0.781
  pilT Acinetobacter baumannii strain A118

78.324

99.712

0.781
  pilT Pseudomonas aeruginosa PAK

77.971

99.424

0.775
  pilT Acinetobacter baylyi ADP1

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Legionella pneumophila strain Lp02

75.221

97.695

0.735
  pilT Legionella pneumophila strain ERS1305867

75.221

97.695

0.735
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Neisseria meningitidis 8013

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.303

95.101

0.669
  pilT Vibrio cholerae strain A1552

70.303

95.101

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.424

0.519
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Vibrio cholerae strain A1552

41.994

95.389

0.401
  pilU Pseudomonas stutzeri DSM 10701

42.901

93.372

0.401