Detailed information NTG0592435

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KY496_RS23085
• Genome accession: NZ_CP080379
• Coordinates: 5224136..5225272 (-)
• Length: 378 a.a.
• NCBI ID: WP_005668051.1
• Uniprot ID: A0A1S2NCK9
• Organism: Massilia sp. NP310
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5219136..5230272

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KY496_RS23075 (KY496_23075)	-	5219403..5221658 (+)	2256	WP_005668048.1	TonB-dependent receptor domain-containing protein	-
KY496_RS23080 (KY496_23080)	-	5221773..5223941 (+)	2169	WP_005668049.1	TonB-dependent siderophore receptor	-
KY496_RS23085 (KY496_23085)	pilU	5224136..5225272 (-)	1137	WP_005668051.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KY496_RS23090 (KY496_23090)	pilT	5225284..5226327 (-)	1044	WP_005668052.1	type IV pilus twitching motility protein PilT	Machinery gene
KY496_RS23095 (KY496_23095)	-	5226402..5227103 (+)	702	WP_040776914.1	YggS family pyridoxal phosphate-dependent enzyme	-
KY496_RS23100 (KY496_23100)	proC	5227115..5227927 (+)	813	WP_005668054.1	pyrroline-5-carboxylate reductase	-
KY496_RS23105 (KY496_23105)	-	5228427..5228894 (-)	468	WP_040776918.1	hypothetical protein	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 41921.34 Da
• Isoelectric Point: 6.7726

>NTDB_id=592435 KY496_RS23085 WP_005668051.1 5224136..5225272(-) (pilU) [Massilia sp. NP310]
MERDQASKFMFDLLRLMVSKGGSDLFITAGFPPAIKIDGRMTPVSSQALTASHTADLARAIMNDKQAAGFELTREANFAI
TPGDLGRFRVSSFMQMGCVGMVLRVITTAIPKFEDLALPDTLKDVVMTKRGLVIMVGATGSGKSTTLAAMVGYRNENSYG
HIITVEDPVEFVHPHRNCVITQREVGVDTDSFEAALKNSLRQAPDVIQIGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGLISQRLIPKKEAKGRVVAIEILLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEADKITYEDALRNADSVNDLRLNIKLNSKHAKNRDFSSGTEKLGLI

Nucleotide

• Length: 1137 bp

>NTDB_id=592435 KY496_RS23085 WP_005668051.1 5224136..5225272(-) (pilU) [Massilia sp. NP310]
ATGGAACGCGACCAGGCATCCAAGTTCATGTTCGACCTGCTGCGCCTGATGGTCAGCAAGGGCGGTTCCGACCTGTTCAT
CACGGCGGGCTTCCCGCCGGCGATCAAGATCGACGGCCGCATGACGCCGGTGTCGAGCCAGGCGCTCACCGCCAGCCACA
CGGCCGACCTGGCGCGCGCGATCATGAACGACAAGCAGGCGGCCGGCTTCGAGCTCACGCGCGAAGCGAACTTCGCGATC
ACGCCGGGCGACCTGGGGCGCTTCCGCGTATCAAGCTTCATGCAGATGGGTTGCGTGGGCATGGTGCTGCGCGTGATCAC
CACCGCGATCCCGAAGTTCGAAGACCTCGCCCTGCCCGACACGCTCAAGGACGTCGTGATGACCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACGGGCTCGGGCAAATCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGAGAACAGCTACGGC
CACATCATCACGGTCGAGGACCCGGTGGAATTCGTCCACCCGCACCGCAACTGCGTGATCACGCAGCGCGAAGTGGGCGT
CGATACCGACAGCTTCGAAGCGGCGCTGAAGAATTCGCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAAATCCGCG
ACCGCGAGACGATGGAACACGCGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTTGACCGCATCATCAACTTCTTCCCTGAAGAGCGCAGACAGCAGCTGCTGATGGACCTGTCGCTGAACCT
CAAGGGCCTGATCTCGCAGCGGCTGATTCCTAAGAAGGAAGCCAAGGGGCGCGTGGTGGCGATCGAGATCCTGCTGAACT
CTCCCTTGATCTCTGACCTGATCTTCAAGGGCGAGGTGCACGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTGGGG
ATGCAGACTTTCGATCAGGCGCTGTTCGACCTGTATGAGGCGGACAAGATCACTTATGAGGATGCGCTGCGCAATGCGGA
TTCGGTGAATGACCTGCGCTTGAACATCAAGCTCAATAGCAAGCACGCGAAGAACCGCGATTTTTCGAGTGGGACGGAGA
AGCTGGGGTTGATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (101-277)

3D structure

• AlphaFold DB: A0A1S2NCK9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	64.47	92.328	0.595
pilU	Acinetobacter baylyi ADP1	58.038	97.09	0.563
pilU	Vibrio cholerae strain A1552	57.303	94.18	0.54
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.373	88.624	0.402
pilT	Acinetobacter nosocomialis M2	43.202	87.566	0.378
pilT	Acinetobacter baylyi ADP1	44.062	84.656	0.373
pilT	Acinetobacter baumannii D1279779	44.164	83.862	0.37
pilT	Acinetobacter baumannii strain A118	44.164	83.862	0.37
pilT	Pseudomonas aeruginosa PAK	41.791	88.624	0.37
pilT	Legionella pneumophila strain ERS1305867	41.692	87.566	0.365
pilT	Legionella pneumophila strain Lp02	41.692	87.566	0.365