Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PCA10_RS01440 Genome accession   NC_021499
Coordinates   310785..311819 (+) Length   344 a.a.
NCBI ID   WP_016490234.1    Uniprot ID   S6AC18
Organism   Metapseudomonas resinovorans NBRC 106553     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 305785..316819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCA10_RS01415 (PCA10_02850) - 305998..307986 (-) 1989 WP_016490229.1 dynamin-like GTPase family protein -
  PCA10_RS01420 (PCA10_02860) - 308196..308492 (-) 297 WP_016490230.1 DUF167 domain-containing protein -
  PCA10_RS01425 (PCA10_02870) - 308492..309085 (-) 594 WP_016490231.1 YggT family protein -
  PCA10_RS01430 (PCA10_02880) proC 309096..309914 (-) 819 WP_016490232.1 pyrroline-5-carboxylate reductase -
  PCA10_RS01435 (PCA10_02890) - 309925..310620 (-) 696 WP_016490233.1 YggS family pyridoxal phosphate-dependent enzyme -
  PCA10_RS01440 (PCA10_02900) pilT 310785..311819 (+) 1035 WP_016490234.1 type IV pilus twitching motility protein PilT Machinery gene
  PCA10_RS01445 (PCA10_02910) pilU 311850..312995 (+) 1146 WP_016490235.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PCA10_RS01450 (PCA10_02920) - 313127..313522 (+) 396 WP_016490236.1 TM2 domain-containing protein -
  PCA10_RS29425 (PCA10_02930) - 313685..313948 (-) 264 WP_016490237.1 hypothetical protein -
  PCA10_RS29430 (PCA10_02940) - 314101..314370 (-) 270 WP_016490238.1 hypothetical protein -
  PCA10_RS30100 (PCA10_02950) - 314556..314879 (-) 324 WP_016490239.1 hypothetical protein -
  PCA10_RS29165 (PCA10_02960) - 314888..315751 (-) 864 WP_016490240.1 RHS repeat-associated core domain-containing protein -
  PCA10_RS29435 (PCA10_02970) - 316301..316675 (-) 375 WP_016490241.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37903.65 Da        Isoelectric Point: 6.8360

>NTDB_id=59186 PCA10_RS01440 WP_016490234.1 310785..311819(+) (pilT) [Metapseudomonas resinovorans NBRC 106553]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEIFKKVSDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLVSKGLVSRESAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=59186 PCA10_RS01440 WP_016490234.1 310785..311819(+) (pilT) [Metapseudomonas resinovorans NBRC 106553]
ATGGACATTACTGAGCTGCTTGCCTTCAGCGCCAAGCAGGGCGCTTCGGACCTGCATCTCTCGGCCGGCCTGCCACCCAT
GATTCGTGTTGACGGCGACGTGCGCCGGATCAACCTGCCAGCCCTGGACCACAAGCAGGTCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTCCCCGGTGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCGGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTGCTGAGCATGGA
AGACCTGGGGATGGGCGAGATCTTCAAGAAGGTTTCCGACGTTCCCCGTGGCCTGGTGCTGGTCACCGGCCCCACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCCATGCTCGACTACCTGAACAGCACCAAGTACCACCACATCCTCACCGTGGAAGAC
CCCATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGATCAACCAGCGGGAAGTGCACCGCGACACCCTGGGCTTCAACGA
AGCCCTGCGCTCGGCCCTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGCCGAGGAAAAGTCCATGGTCCGTTCCATGCTCTCCGAATCCCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGCGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACGGGCGGCTCGCTCGGCATGCAGACCCTCGACATGTGCCTC
AAGGGCCTGGTGAGCAAGGGACTGGTCAGCCGCGAGAGCGCGCGGGAAAAGGCCAAGAGCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S6AC18

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

94.767

100

0.948
  pilT Pseudomonas stutzeri DSM 10701

93.895

100

0.939
  pilT Acinetobacter nosocomialis M2

86.047

100

0.86
  pilT Acinetobacter baumannii D1279779

85.756

100

0.858
  pilT Acinetobacter baumannii strain A118

85.756

100

0.858
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392

Multiple sequence alignment