Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PCA10_RS01445 Genome accession   NC_021499
Coordinates   311850..312995 (+) Length   381 a.a.
NCBI ID   WP_016490235.1    Uniprot ID   S6AL81
Organism   Metapseudomonas resinovorans NBRC 106553     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 306850..317995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCA10_RS01420 (PCA10_02860) - 308196..308492 (-) 297 WP_016490230.1 DUF167 domain-containing protein -
  PCA10_RS01425 (PCA10_02870) - 308492..309085 (-) 594 WP_016490231.1 YggT family protein -
  PCA10_RS01430 (PCA10_02880) proC 309096..309914 (-) 819 WP_016490232.1 pyrroline-5-carboxylate reductase -
  PCA10_RS01435 (PCA10_02890) - 309925..310620 (-) 696 WP_016490233.1 YggS family pyridoxal phosphate-dependent enzyme -
  PCA10_RS01440 (PCA10_02900) pilT 310785..311819 (+) 1035 WP_016490234.1 type IV pilus twitching motility protein PilT Machinery gene
  PCA10_RS01445 (PCA10_02910) pilU 311850..312995 (+) 1146 WP_016490235.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PCA10_RS01450 (PCA10_02920) - 313127..313522 (+) 396 WP_016490236.1 TM2 domain-containing protein -
  PCA10_RS29425 (PCA10_02930) - 313685..313948 (-) 264 WP_016490237.1 hypothetical protein -
  PCA10_RS29430 (PCA10_02940) - 314101..314370 (-) 270 WP_016490238.1 hypothetical protein -
  PCA10_RS30100 (PCA10_02950) - 314556..314879 (-) 324 WP_016490239.1 hypothetical protein -
  PCA10_RS29165 (PCA10_02960) - 314888..315751 (-) 864 WP_016490240.1 RHS repeat-associated core domain-containing protein -
  PCA10_RS29435 (PCA10_02970) - 316301..316675 (-) 375 WP_016490241.1 hypothetical protein -
  PCA10_RS29170 (PCA10_02980) - 316684..317568 (-) 885 WP_016490242.1 RHS repeat-associated core domain-containing protein -
  PCA10_RS01470 (PCA10_02990) - 317594..317941 (-) 348 WP_016490243.1 hypothetical protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42598.92 Da        Isoelectric Point: 6.7261

>NTDB_id=59187 PCA10_RS01445 WP_016490235.1 311850..312995(+) (pilU) [Metapseudomonas resinovorans NBRC 106553]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNPMSPEMTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETTIPTIEELKLPEILKKLSLTKRGLVLFVGATGTGKSTSLAAMIGHRNRNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKALMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLLIKLGSETDGDHLQHMSDGLSLQVTEEDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=59187 PCA10_RS01445 WP_016490235.1 311850..312995(+) (pilU) [Metapseudomonas resinovorans NBRC 106553]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAAAAAGGCGGTTCCGACCTCTTCATCACGGCCGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGACCAAGAACCCCATGTCGCCGGAAATGACCCGGGAAACCGTGCTGGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCGCGAGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGGATCGAGACCACCATCCCGACGATCGA
AGAACTCAAACTGCCGGAAATCCTCAAGAAGCTGTCGCTGACCAAGCGCGGCCTGGTGCTCTTCGTCGGCGCCACCGGTA
CCGGCAAGTCCACGTCCCTGGCGGCGATGATCGGCCACCGCAACCGGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAGCTGCATCGTCACCCAGCGTGAAGTGGGCATCGATACCGAGTCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGGTGCGGTCCCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCCGCCGACCGGCAGCAACAGGTGTGGATGGACCTCTCGCTCAACCTCAAGGCGATCGTCGCCCAGCA
ACTGGTCCCGACCCCCGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTACTGCTCAATACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAAGTGCACGAACTCAAGGCCCTGATGAAACGCTCCACCGAGCAGGGCATGCAGACTTTCGACCAGGCG
CTGTACAACCTCTATTCCCAGGGTGAGATCACCTATGAGGACGCGCTGCTCTACGCCGACTCGGCGAACGACCTGCGACT
GCTGATCAAGCTCGGCTCGGAAACCGACGGCGATCATCTGCAGCACATGAGCGACGGCCTCTCCCTGCAGGTCACCGAGG
AAGACCCCGGGCGCCGCTTCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S6AL81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

90.026

100

0.9
  pilU Acinetobacter baylyi ADP1

63.315

96.588

0.612
  pilU Vibrio cholerae strain A1552

59.143

91.864

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.94

88.189

0.396

Multiple sequence alignment