Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ITG12_RS13560 Genome accession   NZ_CP080238
Coordinates   2906135..2907175 (+) Length   346 a.a.
NCBI ID   WP_038873181.1    Uniprot ID   A0A2N7N1P7
Organism   Vibrio sp. ED002     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2901135..2912175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG12_RS13530 (ITG12_13450) - 2902076..2902678 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  ITG12_RS13535 (ITG12_13455) - 2903050..2903481 (-) 432 WP_038873192.1 DUF4426 domain-containing protein -
  ITG12_RS13540 (ITG12_13460) yggU 2903545..2903835 (-) 291 WP_038873189.1 DUF167 family protein YggU -
  ITG12_RS13545 (ITG12_13465) - 2903835..2904392 (-) 558 WP_248873141.1 YggT family protein -
  ITG12_RS13550 (ITG12_13470) proC 2904447..2905265 (-) 819 WP_042602344.1 pyrroline-5-carboxylate reductase -
  ITG12_RS13555 (ITG12_13475) - 2905396..2906106 (-) 711 WP_038873183.1 YggS family pyridoxal phosphate-dependent enzyme -
  ITG12_RS13560 (ITG12_13480) pilT 2906135..2907175 (+) 1041 WP_038873181.1 type IV pilus twitching motility protein PilT Machinery gene
  ITG12_RS13565 (ITG12_13485) pilU 2907206..2908312 (+) 1107 WP_038873178.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ITG12_RS13570 (ITG12_13490) ruvX 2908536..2908961 (-) 426 WP_038868128.1 Holliday junction resolvase RuvX -
  ITG12_RS13575 (ITG12_13495) - 2909015..2909578 (-) 564 WP_038868129.1 YqgE/AlgH family protein -
  ITG12_RS13580 (ITG12_13500) gshB 2909690..2910640 (-) 951 WP_038881134.1 glutathione synthase -
  ITG12_RS13585 (ITG12_13505) rsmE 2910655..2911386 (-) 732 WP_038868134.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38633.13 Da        Isoelectric Point: 6.5043

>NTDB_id=591578 ITG12_RS13560 WP_038873181.1 2906135..2907175(+) (pilT) [Vibrio sp. ED002]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=591578 ITG12_RS13560 WP_038873181.1 2906135..2907175(+) (pilT) [Vibrio sp. ED002]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCACCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAATTCGAAGAAAAACTCGAAGTCGATTTCTCATTTGAATTGCACAATGTTGGCCGT
TTTCGTGTCAACGCCTTCAACCAATCTCGTGGCTGTGCGGCGGTGTTCCGTACCATCCCAAGTAGCATTCCAACACTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAGATTGCTAACGCAGAAAAAGGGTTAGTACTGGTTACCGGTCCAACAGGTT
CGGGTAAATCGACAACCTTGGCTGCCATTGTTGACTACATTAACCGTAATCACAACAAGCATATTCTTACAATTGAAGAT
CCTATTGAATTTGTTCACAACAACAATAAGTGTTTGATCAACCAACGTGAAGTACACCGTGATACGCATAGCTTCCAAAA
TGCGCTTCGCAGCGCGCTACGTGAAGACCCGGATGTCATTCTAGTTGGTGAGATGCGTGACAAAGAAACTATCAGTTTGG
CGCTGACGGCTGCTGAAACGGGTCACCTTGTTTTCGGTACGCTGCATACTAGCTCAGCAGCGAAAACCATCGACCGTATT
ATCGACGTATTCCCAGGTAGTGACAAAGACATGGTGCGTTCGATGCTGTCGGAGTCGTTGCGTTCGGTTATTGCGCAAAA
GCTGCTTAAGCGAAATGGTGGAGGCCGTATTGCTTGTCATGAGATCATGATGGCAACGCCAGCAATCCGTAACTTGATCC
GCGAAGATAAAGTGGCGCAGATGTACTCGATCATTCAAACCGGTGCGGCACATGGCATGCAAACCATGGAACAAAATGCC
CGTCAGTTGATGGCTCAAGGTATGGTATCGCGTGAAGAAGTCGACAAGAAAGTCGAAATCGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7N1P7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

72.727

95.376

0.694
  pilT Acinetobacter baumannii strain A118

72.727

95.376

0.694
  pilT Acinetobacter nosocomialis M2

72.424

95.376

0.691
  pilT Pseudomonas aeruginosa PAK

69.412

98.266

0.682
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas stutzeri DSM 10701

69.027

97.977

0.676
  pilT Legionella pneumophila strain Lp02

68.278

95.665

0.653
  pilT Legionella pneumophila strain ERS1305867

68.278

95.665

0.653
  pilT Neisseria meningitidis 8013

64.412

98.266

0.633
  pilT Neisseria gonorrhoeae MS11

64.118

98.266

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.143

100

0.506
  pilU Vibrio cholerae strain A1552

41.916

96.532

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.532

0.396
  pilU Acinetobacter baylyi ADP1

39.375

92.486

0.364