Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ITG12_RS13565 Genome accession   NZ_CP080238
Coordinates   2907206..2908312 (+) Length   368 a.a.
NCBI ID   WP_038873178.1    Uniprot ID   A0A2N7N1P0
Organism   Vibrio sp. ED002     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2902206..2913312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG12_RS13535 (ITG12_13455) - 2903050..2903481 (-) 432 WP_038873192.1 DUF4426 domain-containing protein -
  ITG12_RS13540 (ITG12_13460) yggU 2903545..2903835 (-) 291 WP_038873189.1 DUF167 family protein YggU -
  ITG12_RS13545 (ITG12_13465) - 2903835..2904392 (-) 558 WP_248873141.1 YggT family protein -
  ITG12_RS13550 (ITG12_13470) proC 2904447..2905265 (-) 819 WP_042602344.1 pyrroline-5-carboxylate reductase -
  ITG12_RS13555 (ITG12_13475) - 2905396..2906106 (-) 711 WP_038873183.1 YggS family pyridoxal phosphate-dependent enzyme -
  ITG12_RS13560 (ITG12_13480) pilT 2906135..2907175 (+) 1041 WP_038873181.1 type IV pilus twitching motility protein PilT Machinery gene
  ITG12_RS13565 (ITG12_13485) pilU 2907206..2908312 (+) 1107 WP_038873178.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ITG12_RS13570 (ITG12_13490) ruvX 2908536..2908961 (-) 426 WP_038868128.1 Holliday junction resolvase RuvX -
  ITG12_RS13575 (ITG12_13495) - 2909015..2909578 (-) 564 WP_038868129.1 YqgE/AlgH family protein -
  ITG12_RS13580 (ITG12_13500) gshB 2909690..2910640 (-) 951 WP_038881134.1 glutathione synthase -
  ITG12_RS13585 (ITG12_13505) rsmE 2910655..2911386 (-) 732 WP_038868134.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ITG12_RS13590 (ITG12_13510) endA 2911534..2912229 (-) 696 WP_038881140.1 deoxyribonuclease I -
  ITG12_RS13595 (ITG12_13515) - 2912380..2912877 (-) 498 WP_038868139.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41312.46 Da        Isoelectric Point: 6.7730

>NTDB_id=591579 ITG12_RS13565 WP_038873178.1 2907206..2908312(+) (pilU) [Vibrio sp. ED002]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLSENDVAMLLDSAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKTTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=591579 ITG12_RS13565 WP_038873178.1 2907206..2908312(+) (pilU) [Vibrio sp. ED002]
ATGGATCTGAATAAATTTCTCGAAGGCATGCTGGCGTTAAAAGCGTCGGATCTTTATATCACGGTTGGTGCGCCGATCTT
ATTTCGTGTGGATGGCGAACTGCGTCCGCAAGGGGATAAGTTGAGTGAAAATGACGTAGCCATGCTGCTCGATAGTGCAA
TGGATCCAGAGCGACGTCAGGAGTTTCGTAAAAGTCGAGAATCAAACTTTGCGATTGTCCGAGATTGTGGCCGCTTCCGT
GTTAGTGCTTTCTTTCAGCGAGAATTACCTGGTGCGGTGATTCGTCGTATCGAAACCAACATTCCTACGTTTGAACAGCT
TAAGCTGCCTTTAGTGTTGCAAGATCTTGCGATAGCGAAACGTGGCTTAGTGCTGGTGGTTGGTGCTACAGGTTCCGGTA
AGTCGACCACTATGGCGGCGATGACGGGTTACCGTAACAGTAATAAAACAGGTCACATTCTGACAGTTGAAGACCCAATC
GAATTTGTGCACGAGCACAAGCGTTGTATCGTGACTCAGCGTGAAGTGGGATTGGATACCGACAGCTATGAAGTCGCGTT
AAAGAACTCATTACGCCAAGCGCCTGACATGATTTTGATTGGTGAGATCCGTAGCCGCGAAACGATGGAATACGCAATGA
CCTTTGCCGAGACTGGTCACTTGTGTATGGCTACATTGCACGCGAATAACGCAAACCAAGCACTTGAGCGTATTTTGCAC
CTAGTGCCAAAAGATCAGAAAGATCAGTTCCTGTTTGATCTGTCGATGAACTTAAAAGGCGTAGTAGGTCAACAGCTGAT
CCGTGATAAAAACGGTCAAGGACGTCATGGCGTGTTTGAGATTCTGCTAAATAGTCCACGAGTTTCTGATCTGATCCGTC
GCGGTGATCTGCATGAGTTAAAAACCACCATGGCACGCTCTAATGAATTCGGGATGCTGACGTTTGATCAGTCGCTCTAT
AAATTGGTGATGCAAGGCAAGATCAGTGAAGAAGATGCATTGCACAGTGCGGATTCAGCTAACGATCTGCGTTTGATGTT
GAAGACGCAACGCGGTGAAGCTTTCTCTACAGGCACGCTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7N1P0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.793

100

0.818
  pilU Pseudomonas stutzeri DSM 10701

59.143

95.109

0.563
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.279

93.478

0.386
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

40.351

92.935

0.375
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37