Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV822_RS09670 Genome accession   NZ_CP079801
Coordinates   2103168..2104202 (+) Length   344 a.a.
NCBI ID   WP_238869790.1    Uniprot ID   -
Organism   Halopseudomonas maritima strain RR6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2098168..2109202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV822_RS09640 (HV822_09625) - 2098599..2099030 (-) 432 WP_238869785.1 DUF4426 domain-containing protein -
  HV822_RS09645 (HV822_09630) metW 2099054..2099644 (-) 591 WP_238873578.1 methionine biosynthesis protein MetW -
  HV822_RS09650 (HV822_09635) - 2099647..2100789 (-) 1143 WP_238869786.1 homoserine O-acetyltransferase -
  HV822_RS09655 (HV822_09640) - 2100961..2101551 (-) 591 WP_238869787.1 YggT family protein -
  HV822_RS09660 (HV822_09645) proC 2101561..2102379 (-) 819 WP_238869788.1 pyrroline-5-carboxylate reductase -
  HV822_RS09665 (HV822_09650) - 2102403..2103089 (-) 687 WP_238869789.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV822_RS09670 (HV822_09655) pilT 2103168..2104202 (+) 1035 WP_238869790.1 type IV pilus twitching motility protein PilT Machinery gene
  HV822_RS09675 (HV822_09660) pilU 2104264..2105418 (+) 1155 WP_238869791.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HV822_RS09680 (HV822_09665) siaD 2105585..2106376 (-) 792 WP_238869792.1 biofilm regulation diguanylate cyclase SiaD -
  HV822_RS09685 (HV822_09670) siaC 2106384..2106761 (-) 378 WP_238869793.1 biofilm regulation phosphoprotein SiaC -
  HV822_RS09690 (HV822_09675) siaB 2106783..2107325 (-) 543 WP_238869794.1 biofilm regulation protein kinase SiaB -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37794.62 Da        Isoelectric Point: 6.7750

>NTDB_id=589561 HV822_RS09670 WP_238869790.1 2103168..2104202(+) (pilT) [Halopseudomonas maritima strain RR6]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINVPAMEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMSDVFKKISDNPRGLVLVTGPTGSGKSTSLAAMVDYLNSTKYQHILTIED
PIEFVHESKKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGEEKAMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKIAQMYSAIQTGGNIGMQTLDMCL
KGLVAKGLITKESAREKAKAVDGF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=589561 HV822_RS09670 WP_238869790.1 2103168..2104202(+) (pilT) [Halopseudomonas maritima strain RR6]
ATGGATATTACCGAGCTGTTAGCCTTCAGCGCCAAACAGGGCGCGTCGGACTTGCATCTGTCGGCAGGATTGCCGCCCAT
GATTCGCGTCGACGGCGACGTGCGCCGAATCAACGTGCCGGCCATGGAGCACAAGCAGGTTCATGCGTTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTTGAAGAGTTCCTGGAGACGGACTTCTCCTTTGAAGTGCCGGGTGTCGCGCGC
TTCCGGGTTAACGCCTTCAACCAGAACCGGGGCGCTGGCGCCGTATTCCGGACCATTCCTTCCAAGGTACTGACCATGGA
AGATCTGGGTATGAGCGATGTGTTCAAGAAGATTTCCGACAACCCGCGCGGTCTGGTGCTGGTCACCGGCCCGACCGGCT
CGGGTAAATCCACCAGCCTGGCGGCCATGGTGGACTACCTCAACAGCACCAAGTACCAGCATATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACGAGTCCAAGAAGTGTCTGGTGAACCAGCGTGAAGTACACCGCGATACCCTGGGCTTTGCCGA
GGCCCTGCGTTCGGCCCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGTGACCTTGAAACCATTCGCCTGG
CGTTGACCGCCGCAGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGGCGAAGAAAAAGCCATGGTGCGCTCGATGCTGTCCGAATCGCTGCAGGCGGTGATCTCCCAAAC
CCTGCTGAAGAAGATTGGCGGCGGCCGGGTGGCGGCGCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAAGACAAGATCGCCCAGATGTACTCGGCCATTCAGACCGGCGGCAACATTGGCATGCAGACACTCGACATGTGCCTG
AAAGGGCTGGTCGCCAAGGGTCTGATCACCAAGGAAAGCGCCCGCGAAAAGGCCAAGGCAGTCGACGGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

91.86

100

0.919
  pilT Pseudomonas stutzeri DSM 10701

90.988

100

0.91
  pilT Acinetobacter baumannii D1279779

82.849

100

0.828
  pilT Acinetobacter baumannii strain A118

82.849

100

0.828
  pilT Acinetobacter nosocomialis M2

82.558

100

0.826
  pilT Acinetobacter baylyi ADP1

80.523

100

0.805
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Neisseria meningitidis 8013

68.529

98.837

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria gonorrhoeae MS11

68.235

98.837

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.097

98.547

0.523
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

40.233

99.709

0.401
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39