Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KYK33_RS03455 Genome accession   NZ_CP079742
Coordinates   732378..733412 (+) Length   344 a.a.
NCBI ID   WP_224432034.1    Uniprot ID   -
Organism   Aeromonas rivuli strain 20-VB00005     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 727378..738412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KYK33_RS03415 (KYK33_03415) - 727705..728190 (-) 486 WP_042041607.1 Rsd/AlgQ family anti-sigma factor -
  KYK33_RS03420 (KYK33_03420) hupA 728528..728800 (+) 273 WP_005305063.1 nucleoid-associated protein HU-alpha -
  KYK33_RS03425 (KYK33_03425) - 728903..729328 (+) 426 WP_042041606.1 hypothetical protein -
  KYK33_RS03430 (KYK33_03430) - 729401..729817 (-) 417 WP_042041605.1 DUF4426 domain-containing protein -
  KYK33_RS03435 (KYK33_03435) yggU 729839..730141 (-) 303 WP_224432031.1 DUF167 family protein YggU -
  KYK33_RS03440 (KYK33_03440) - 730141..730692 (-) 552 WP_042041603.1 YggT family protein -
  KYK33_RS03445 (KYK33_03445) proC 730706..731530 (-) 825 WP_224432032.1 pyrroline-5-carboxylate reductase -
  KYK33_RS03450 (KYK33_03450) - 731637..732338 (-) 702 WP_224432033.1 YggS family pyridoxal phosphate-dependent enzyme -
  KYK33_RS03455 (KYK33_03455) pilT 732378..733412 (+) 1035 WP_224432034.1 type IVa pilus ATPase TapT Machinery gene
  KYK33_RS03460 (KYK33_03460) pilU 733421..734530 (+) 1110 WP_224432035.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KYK33_RS03465 (KYK33_03465) yaaA 734565..735338 (+) 774 WP_042041596.1 peroxide stress protein YaaA -
  KYK33_RS03470 (KYK33_03470) srmB 735572..736795 (-) 1224 WP_224432036.1 ATP-dependent RNA helicase SrmB -
  KYK33_RS03475 (KYK33_03475) - 736915..737625 (+) 711 WP_224432037.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38202.85 Da        Isoelectric Point: 6.5053

>NTDB_id=589106 KYK33_RS03455 WP_224432034.1 732378..733412(+) (pilT) [Aeromonas rivuli strain 20-VB00005]
MDITELLAFSVKHNASDLHLSAGVPPMIRVDGEVRKINLPALDQREVHALIYDIMNDHQRKELEEHFEVDFSFEVPNMAR
FRVNAFQQSRGAGAVFRTIPSTVLSLEELDAPDIFQKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMVHGMQTMDQSL
KQLVGRGTVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=589106 KYK33_RS03455 WP_224432034.1 732378..733412(+) (pilT) [Aeromonas rivuli strain 20-VB00005]
ATGGATATCACAGAGTTATTGGCCTTTAGTGTAAAACATAATGCCTCGGACCTACACCTCTCGGCCGGTGTGCCACCGAT
GATCAGGGTTGATGGCGAGGTGCGCAAGATCAATTTGCCAGCCCTGGATCAGCGAGAGGTGCACGCCCTTATCTATGACA
TCATGAACGACCATCAGCGCAAGGAACTGGAAGAGCATTTTGAGGTCGATTTCTCGTTTGAGGTGCCCAACATGGCACGC
TTCCGGGTCAACGCCTTCCAGCAATCCCGCGGCGCTGGCGCAGTGTTCCGCACCATCCCCAGCACGGTGTTGAGTCTGGA
AGAGCTCGATGCCCCCGATATTTTTCAGAAGATTGCCGAATATCCACGGGGTCTGGTGCTGGTGACCGGGCCAACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGGTCAATTACATCAACGAGAACTTCCATCACCATATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAAAACAAGCGCTGCCTGGTCAACCAGCGTGAGGTGCACAGGGACACCAAGAGTTTTTCCAA
TGCCCTGCGCTCAGCGCTGCGGGAGGATCCGGACATCATACTGGTGGGGGAGATGCGCGATCTGGAAACCATACGCCTCG
CCATGACGGCGGCTGAGACCGGTCATCTGGTGTTTGGCACCCTGCACACCTCCTCGGCGGCCAAAACCATAGACCGCATC
ATCGATGTCTTCCCCGGTGCGGAGAAAGACATGGTGCGATCCATGCTGTCCGAGTCACTGCGAGCCGTTATCTCCCAGAC
GCTGCTCAAGCGCATTGGGGGCGGACGGGTCGCTGCCCACGAAATCATGCTGGGGATACCGGCGGTGCGTAACCTTATCC
GTGAAGACAAGGTGGCCCAGCTCTACTCCGTTATCCAGACAGGGATGGTGCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGGGGCGCGGGACAGTGGCCTCCCTGGATGCCAAGGCCAAGGCGGTGGATCCCAATTCTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Acinetobacter nosocomialis M2

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

75.872

100

0.759
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

71.176

98.837

0.703
  pilT Legionella pneumophila strain ERS1305867

71.176

98.837

0.703
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.187

99.709

0.52
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.41
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401