Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KYK33_RS03460 Genome accession   NZ_CP079742
Coordinates   733421..734530 (+) Length   369 a.a.
NCBI ID   WP_224432035.1    Uniprot ID   -
Organism   Aeromonas rivuli strain 20-VB00005     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 728421..739530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KYK33_RS03420 (KYK33_03420) hupA 728528..728800 (+) 273 WP_005305063.1 nucleoid-associated protein HU-alpha -
  KYK33_RS03425 (KYK33_03425) - 728903..729328 (+) 426 WP_042041606.1 hypothetical protein -
  KYK33_RS03430 (KYK33_03430) - 729401..729817 (-) 417 WP_042041605.1 DUF4426 domain-containing protein -
  KYK33_RS03435 (KYK33_03435) yggU 729839..730141 (-) 303 WP_224432031.1 DUF167 family protein YggU -
  KYK33_RS03440 (KYK33_03440) - 730141..730692 (-) 552 WP_042041603.1 YggT family protein -
  KYK33_RS03445 (KYK33_03445) proC 730706..731530 (-) 825 WP_224432032.1 pyrroline-5-carboxylate reductase -
  KYK33_RS03450 (KYK33_03450) - 731637..732338 (-) 702 WP_224432033.1 YggS family pyridoxal phosphate-dependent enzyme -
  KYK33_RS03455 (KYK33_03455) pilT 732378..733412 (+) 1035 WP_224432034.1 type IVa pilus ATPase TapT Machinery gene
  KYK33_RS03460 (KYK33_03460) pilU 733421..734530 (+) 1110 WP_224432035.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KYK33_RS03465 (KYK33_03465) yaaA 734565..735338 (+) 774 WP_042041596.1 peroxide stress protein YaaA -
  KYK33_RS03470 (KYK33_03470) srmB 735572..736795 (-) 1224 WP_224432036.1 ATP-dependent RNA helicase SrmB -
  KYK33_RS03475 (KYK33_03475) - 736915..737625 (+) 711 WP_224432037.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  KYK33_RS03480 (KYK33_03480) brnQ 737861..739123 (+) 1263 WP_263480042.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41164.44 Da        Isoelectric Point: 6.1100

>NTDB_id=589107 KYK33_RS03460 WP_224432035.1 733421..734530(+) (pilU) [Aeromonas rivuli strain 20-VB00005]
MKLEMLLVQLVERQGSDLFITVGSRPMVKVDGHLHPLDDRVFDEEQVLALIRETLSPEHLAHFMATQEANYAIHRPELGR
FRVSAFWQQALPGMVLRRIETRIPTFEELGLPDILKEIGMAKRGLVLFVGATGTGKTTTQAAMIGYRNQHSDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDAALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQAIDRI
LHLVPPEKHRQLLFDLSFNLRGIIAQQLLPALDGQRRCPVFEVMLNTPIITDIIRKGEVHRLKEVMTKSTELGMQTFDQG
LFQLFMAGRIGLAEALAHADSANDLRLQIKLSGKDPLDSGTLEGVTVDD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=589107 KYK33_RS03460 WP_224432035.1 733421..734530(+) (pilU) [Aeromonas rivuli strain 20-VB00005]
ATGAAACTCGAGATGTTGTTGGTACAGCTGGTCGAGCGACAAGGCTCGGATTTGTTCATCACAGTGGGCAGTCGCCCCAT
GGTCAAGGTAGACGGTCACCTGCACCCCCTCGACGATAGGGTGTTCGATGAGGAGCAGGTGCTTGCCTTGATCCGGGAGA
CCCTGAGCCCGGAACATCTCGCGCATTTCATGGCGACCCAGGAGGCAAACTACGCCATTCACCGACCTGAGCTGGGGCGA
TTTCGGGTCAGCGCCTTCTGGCAGCAGGCCCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGGATCCCGACGTTTGA
GGAGCTGGGCTTGCCCGATATCCTGAAAGAGATCGGCATGGCCAAGCGGGGGCTGGTGTTGTTTGTCGGGGCGACGGGAA
CCGGGAAAACCACTACCCAGGCGGCCATGATTGGTTATCGCAACCAGCACAGCGATGGGCATATCCTGACGGTGGAGGAT
CCGGTGGAATTCGTGCACCAGCATGGCCGCAGCCTCATCACCCAGCGGGAGGTGGGCATTGATACGGCCTCTTTCGATGC
CGCCCTCAAGAGTTCCTTGCGTCAGGCTCCGGACGTCATCCTCATCGGCGAGATCCGCAGTCAGGAGACCATGGAGTTCG
CACTGCAATTTGCCGAGACGGGTCACCTCTGCCTCGCGACCCTGCATGCCAACAATGCCAATCAGGCCATAGATCGCATC
CTGCATCTGGTGCCGCCCGAGAAGCATCGTCAACTGCTGTTCGATCTCTCCTTCAACCTGAGAGGCATCATTGCCCAACA
GCTGTTGCCTGCCCTCGATGGCCAGCGGCGCTGCCCTGTGTTCGAGGTGATGCTCAATACTCCCATCATCACCGACATCA
TCCGCAAGGGAGAAGTGCATCGCCTCAAGGAGGTGATGACCAAATCGACCGAGCTGGGCATGCAGACCTTCGATCAGGGG
CTCTTCCAGCTATTCATGGCCGGCCGCATCGGCCTCGCGGAGGCGTTGGCCCACGCGGATTCCGCCAATGACTTGCGCCT
GCAGATCAAGTTATCGGGCAAGGATCCTCTGGATTCTGGTACGCTGGAGGGCGTAACCGTAGATGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.967

99.729

0.588
  pilU Pseudomonas stutzeri DSM 10701

57.91

95.935

0.556
  pilU Acinetobacter baylyi ADP1

54.696

98.103

0.537
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.515

0.382
  pilT Vibrio cholerae strain A1552

42.216

90.515

0.382
  pilT Acinetobacter baylyi ADP1

41.349

92.412

0.382
  pilT Pseudomonas stutzeri DSM 10701

39.244

93.225

0.366