Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KW115_RS15280 Genome accession   NZ_CP079095
Coordinates   3157706..3158740 (-) Length   344 a.a.
NCBI ID   WP_218806521.1    Uniprot ID   -
Organism   Methylococcus sp. Mc7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3152706..3163740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW115_RS15255 (KW115_15255) - 3153292..3154074 (+) 783 WP_255556728.1 succinate dehydrogenase iron-sulfur subunit -
  KW115_RS15260 (KW115_15260) - 3154071..3154328 (+) 258 WP_218806517.1 succinate dehydrogenase assembly factor 2 -
  KW115_RS15265 (KW115_15265) - 3154322..3154744 (+) 423 WP_218806518.1 protein YgfX -
  KW115_RS15270 (KW115_15270) - 3154755..3156536 (+) 1782 WP_218806519.1 VWA domain-containing protein -
  KW115_RS15275 (KW115_15275) pilU 3156570..3157691 (-) 1122 WP_218806520.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KW115_RS15280 (KW115_15280) pilT 3157706..3158740 (-) 1035 WP_218806521.1 type IV pilus twitching motility protein PilT Machinery gene
  KW115_RS15285 (KW115_15285) - 3158794..3159483 (+) 690 WP_218806522.1 YggS family pyridoxal phosphate-dependent enzyme -
  KW115_RS15290 (KW115_15290) proC 3159554..3160378 (+) 825 WP_218806523.1 pyrroline-5-carboxylate reductase -
  KW115_RS15295 (KW115_15295) - 3160382..3160960 (+) 579 WP_218806524.1 YggT family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38292.98 Da        Isoelectric Point: 6.7754

>NTDB_id=587640 KW115_RS15280 WP_218806521.1 3157706..3158740(-) (pilT) [Methylococcus sp. Mc7]
MDIAELLAFSVKNKSSDLHLSSGLPPMIRVDGDIRRINVPPLEHKEVHALVYDIMNDKQRRDFEEFLETDFSFELPGVAR
FRVNAFNQDRGAAAVFRTIPSKVLTLEELGCPKFFQDVTRQPRGLIVVTGPTGSGKSTTLAAMIDYINSNDYSHILTIED
PIEFVHQSKKCLINQREVHRNTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMIRSMLSESLQAVISQALVKKAGGGRTAAWEIMVGTPAIRNLIREAKVAQMYSTIQTGRKDGMQTLDQHL
QELVEKGVITRQVAREIAVNKAAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=587640 KW115_RS15280 WP_218806521.1 3157706..3158740(-) (pilT) [Methylococcus sp. Mc7]
ATGGATATCGCCGAACTGCTCGCCTTCTCGGTCAAAAACAAATCCTCAGACCTGCACCTTTCCTCGGGGCTGCCGCCGAT
GATCCGCGTCGACGGCGACATCCGGCGCATCAACGTGCCGCCGCTGGAGCACAAGGAAGTCCACGCCCTGGTCTATGACA
TCATGAACGACAAGCAGCGGCGCGACTTCGAGGAATTCCTGGAAACCGACTTCTCGTTCGAACTGCCCGGCGTCGCGCGT
TTCCGCGTCAACGCCTTCAACCAGGACCGGGGCGCGGCGGCCGTGTTCCGGACCATTCCATCCAAAGTCCTCACGCTGGA
GGAACTGGGCTGCCCGAAATTCTTCCAGGACGTCACCCGCCAGCCGCGCGGACTGATCGTGGTCACCGGACCCACGGGGT
CGGGCAAGTCCACCACGCTGGCGGCCATGATCGACTACATCAACTCCAACGACTATTCCCACATCCTGACCATCGAAGAC
CCCATCGAATTCGTGCACCAAAGCAAGAAATGCCTGATCAACCAGCGCGAGGTGCACCGCAACACCTTGGGCTTCAACGA
AGCCTTGCGCTCGGCACTGCGCGAAGACCCGGACGTGATCCTGGTGGGTGAAATGCGCGATCTCGAAACCATCCGCCTGG
CGCTCACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCCGCCGCCAAGACCATCGACCGCATC
GTCGACGTATTCCCCGCCGCGGAAAAGGAGATGATCCGCTCCATGCTGTCGGAATCGCTGCAAGCCGTGATTTCGCAGGC
GCTCGTCAAGAAAGCCGGCGGCGGCCGGACCGCGGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GGGAGGCCAAAGTCGCCCAGATGTACTCGACGATCCAGACCGGCCGCAAGGACGGCATGCAGACCCTGGACCAGCACCTC
CAGGAACTGGTGGAAAAAGGGGTCATCACCCGCCAGGTCGCCCGCGAAATCGCCGTCAACAAGGCCGCGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.065

98.256

0.797
  pilT Pseudomonas stutzeri DSM 10701

78.779

100

0.788
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Acinetobacter baylyi ADP1

76.744

100

0.767
  pilT Legionella pneumophila strain Lp02

74.419

100

0.744
  pilT Legionella pneumophila strain ERS1305867

74.419

100

0.744
  pilT Neisseria meningitidis 8013

68.406

100

0.686
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.667

100

0.669
  pilT Vibrio cholerae strain A1552

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

39.157

96.512

0.378