Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KW115_RS15275 Genome accession   NZ_CP079095
Coordinates   3156570..3157691 (-) Length   373 a.a.
NCBI ID   WP_218806520.1    Uniprot ID   -
Organism   Methylococcus sp. Mc7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3151570..3162691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW115_RS15250 (KW115_15250) - 3151850..3153259 (+) 1410 WP_218806516.1 aspartate ammonia-lyase -
  KW115_RS15255 (KW115_15255) - 3153292..3154074 (+) 783 WP_255556728.1 succinate dehydrogenase iron-sulfur subunit -
  KW115_RS15260 (KW115_15260) - 3154071..3154328 (+) 258 WP_218806517.1 succinate dehydrogenase assembly factor 2 -
  KW115_RS15265 (KW115_15265) - 3154322..3154744 (+) 423 WP_218806518.1 protein YgfX -
  KW115_RS15270 (KW115_15270) - 3154755..3156536 (+) 1782 WP_218806519.1 VWA domain-containing protein -
  KW115_RS15275 (KW115_15275) pilU 3156570..3157691 (-) 1122 WP_218806520.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KW115_RS15280 (KW115_15280) pilT 3157706..3158740 (-) 1035 WP_218806521.1 type IV pilus twitching motility protein PilT Machinery gene
  KW115_RS15285 (KW115_15285) - 3158794..3159483 (+) 690 WP_218806522.1 YggS family pyridoxal phosphate-dependent enzyme -
  KW115_RS15290 (KW115_15290) proC 3159554..3160378 (+) 825 WP_218806523.1 pyrroline-5-carboxylate reductase -
  KW115_RS15295 (KW115_15295) - 3160382..3160960 (+) 579 WP_218806524.1 YggT family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41675.08 Da        Isoelectric Point: 6.4191

>NTDB_id=587639 KW115_RS15275 WP_218806520.1 3156570..3157691(-) (pilU) [Methylococcus sp. Mc7]
MEFESLLKLMVLKKASDLFITAAKEPCMKVNGTIVPLSSTKLSAEQVRQLVLGIMNQRQRDEFENTNECNFALSAAGLGR
FRVSAFVQRNSPGMVLRRIETEIPTVEQLDLPSILNDLVMTKRGLILFVGATGTGKSTSLAALLKYRNEHSSGHIITIED
PLEFVHPHAGCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMQQAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHPQLFMDLSLNLRAIIAQQLVKRADGKGRYPAVEILINTPLVSDLIRQGEVHKLKEVMKQSRELGMQTFDQA
LFELFKAGRIGYEDALYSADSKNEVRLMIKLSEQGGIDKYAPKDDSIQIVANE

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=587639 KW115_RS15275 WP_218806520.1 3156570..3157691(-) (pilU) [Methylococcus sp. Mc7]
ATGGAATTCGAATCGCTGCTCAAACTGATGGTGCTGAAGAAAGCGTCCGATCTCTTCATCACCGCGGCCAAGGAGCCCTG
CATGAAGGTCAACGGCACCATCGTGCCGCTGTCCAGCACCAAACTGAGCGCCGAACAGGTGCGCCAGCTCGTCCTCGGCA
TCATGAACCAGCGCCAGCGCGACGAGTTCGAAAACACCAACGAATGCAACTTCGCCCTCTCGGCGGCCGGGCTGGGCCGG
TTCCGGGTCAGCGCCTTCGTCCAGCGGAACAGTCCCGGCATGGTGCTGCGCCGGATCGAGACCGAGATTCCCACCGTGGA
ACAGCTCGATCTGCCGTCCATCCTCAACGACCTGGTCATGACCAAGCGCGGCCTGATCCTGTTCGTGGGCGCGACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGTTGCTCAAGTACCGCAACGAGCACTCCAGCGGCCACATCATCACCATCGAGGAT
CCCCTGGAGTTCGTACACCCGCACGCGGGCTGCATCGTCACCCAGCGCGAGGTCGGCATCGACACCGAATCCTACGAAGT
CGCGCTGAAGAACACTCTGCGGCAGGCACCGGACGTGATCCTCATCGGCGAGATCCGCACCCGCGAGACCATGCAGCAGG
CCATCACCTTCGCCGAAACCGGCCATCTCTGTCTGAGCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
CTCCATTTCTTTCCGGAAGACATGCATCCGCAGCTGTTCATGGACCTGTCGCTGAACCTCCGCGCCATCATCGCCCAGCA
GCTCGTCAAGCGCGCCGACGGCAAGGGACGCTATCCGGCGGTGGAAATCCTCATCAACACGCCCCTGGTCTCCGACCTGA
TCCGCCAAGGAGAGGTCCACAAGCTCAAGGAGGTCATGAAGCAGTCGCGCGAACTGGGCATGCAGACCTTCGACCAGGCG
CTGTTCGAACTGTTCAAGGCGGGCAGGATCGGCTACGAGGATGCCCTCTACTCCGCCGACTCCAAGAACGAAGTGCGCTT
GATGATCAAGCTCAGCGAACAGGGCGGCATCGACAAATACGCGCCCAAGGACGACTCCATCCAGATCGTCGCCAACGAAT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.492

94.906

0.66
  pilU Acinetobacter baylyi ADP1

64.706

95.71

0.619
  pilU Vibrio cholerae strain A1552

57.714

93.834

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.103

93.298

0.402
  pilT Legionella pneumophila strain Lp02

40.816

91.957

0.375
  pilT Legionella pneumophila strain ERS1305867

40.816

91.957

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

89.544

0.373
  pilT Vibrio cholerae strain A1552

41.617

89.544

0.373
  pilT Acinetobacter baylyi ADP1

40.176

91.421

0.367
  pilT Pseudomonas aeruginosa PAK

40.597

89.812

0.365
  pilT Acinetobacter nosocomialis M2

39.823

90.885

0.362
  pilT Acinetobacter baumannii D1279779

39.823

90.885

0.362
  pilT Acinetobacter baumannii strain A118

39.823

90.885

0.362