Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KUD94_RS13230 Genome accession   NZ_CP078069
Coordinates   2747774..2748817 (+) Length   347 a.a.
NCBI ID   WP_218237647.1    Uniprot ID   -
Organism   Comamonas sp. NLF-1-9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2742774..2753817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUD94_RS13210 (KUD94_13210) - 2742921..2744357 (+) 1437 WP_218237643.1 DUF2868 domain-containing protein -
  KUD94_RS13215 (KUD94_13215) - 2744354..2745763 (+) 1410 WP_218237644.1 GTPase/DUF3482 domain-containing protein -
  KUD94_RS13220 (KUD94_13220) - 2745778..2746914 (-) 1137 WP_218237645.1 aminotransferase class V-fold PLP-dependent enzyme -
  KUD94_RS13225 (KUD94_13225) - 2746965..2747726 (-) 762 WP_218237646.1 YggS family pyridoxal phosphate-dependent enzyme -
  KUD94_RS13230 (KUD94_13230) pilT 2747774..2748817 (+) 1044 WP_218237647.1 type IV pilus twitching motility protein PilT Machinery gene
  KUD94_RS13235 (KUD94_13235) - 2748899..2749528 (+) 630 WP_218237648.1 cyclic nucleotide-binding domain-containing protein -
  KUD94_RS13240 (KUD94_13240) pilU 2749571..2750707 (+) 1137 WP_146912251.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KUD94_RS13245 (KUD94_13245) - 2750725..2751660 (+) 936 WP_218237649.1 NAD(P)-dependent oxidoreductase -
  KUD94_RS13250 (KUD94_13250) - 2751705..2752415 (-) 711 WP_218237650.1 BON domain-containing protein -
  KUD94_RS13255 (KUD94_13255) - 2752618..2753784 (-) 1167 WP_218237651.1 phospholipase A -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38175.87 Da        Isoelectric Point: 6.6647

>NTDB_id=586551 KUD94_RS13230 WP_218237647.1 2747774..2748817(+) (pilT) [Comamonas sp. NLF-1-9]
MDITQLLAFSVKNNASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKIYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTIED
PIEFVHESRKCLVNQREVGPMTLSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLCKLKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTGNSQGMQTLDQN
LTELVRRNVISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=586551 KUD94_RS13230 WP_218237647.1 2747774..2748817(+) (pilT) [Comamonas sp. NLF-1-9]
GTGGACATCACCCAGCTACTAGCCTTCAGCGTGAAGAACAACGCCTCGGACCTGCACCTGTCCGCAGGTCTGCCGCCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAATGTCGATGCGCTCGATCACAAGACGGTACACGCCATGGTGTACGACA
TCATGAGCGACGCGCAGCGCAAGATCTACGAAGAATTCCTCGAGGTGGACTTCTCCTTCGAGATCGACGGCCTGGCGCGT
TTTCGCGTCAATGCCTTCAACCAGAACCGCGGCGCGGCGGCGGTGTTCCGCACCATTCCGAGCAAAATTCTCACGCTCGA
GCAGCTCAACGCTCCCAAGATTTTTGCCGACCTGGCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACGGGTT
CGGGCAAGTCCACGACGCTGGCGGCCATGGTCAACCACCTGAACGAAACCGAGTACGGCCACATCCTGACCATCGAGGAC
CCGATCGAGTTCGTGCACGAATCGCGCAAGTGCCTGGTGAACCAGCGCGAAGTCGGGCCGATGACGCTGTCCTTTTCGGC
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCCATCCTGGTCGGTGAGATGCGCGACCTCGAAACCATACGCCTGG
CGATGACGGCCGCCGAAACCGGGCACCTGGTGTTCGGCACGCTGCACACGTCTTCGGCGGCCAAGACCATAGACCGGGTC
ATCGACGTCTTTCCGGCCGAGGAAAAGGACATGGTGCGCGCCATGCTGTCCGAGTCGCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGCTCAAAGACGGCTCGGGCCGTGTGGCCGCGCACGAAATCATGCTGGGCACCAGCGCGATCCGCAACCTGA
TCCGCGAGGCCAAGGTCGCACAGATGTATTCGACCATCCAGACCGGCAACAGCCAGGGCATGCAGACGCTGGACCAGAAC
CTCACCGAGCTGGTGCGACGCAACGTGATCAGCCCGGCCGAGGCGCGCAGCAAGGCCAAGATACCGGAGAATTTCCCCGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.971

99.424

0.775
  pilT Pseudomonas stutzeri DSM 10701

77.391

99.424

0.769
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Vibrio cholerae strain A1552

68.622

98.271

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.622

98.271

0.674
  pilT Neisseria meningitidis 8013

66.957

99.424

0.666
  pilT Neisseria gonorrhoeae MS11

66.667

99.424

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395
  pilU Acinetobacter baylyi ADP1

39.595

99.712

0.395