Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KUD94_RS13240 Genome accession   NZ_CP078069
Coordinates   2749571..2750707 (+) Length   378 a.a.
NCBI ID   WP_146912251.1    Uniprot ID   A0A5B8RSY7
Organism   Comamonas sp. NLF-1-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2744571..2755707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUD94_RS13220 (KUD94_13220) - 2745778..2746914 (-) 1137 WP_218237645.1 aminotransferase class V-fold PLP-dependent enzyme -
  KUD94_RS13225 (KUD94_13225) - 2746965..2747726 (-) 762 WP_218237646.1 YggS family pyridoxal phosphate-dependent enzyme -
  KUD94_RS13230 (KUD94_13230) pilT 2747774..2748817 (+) 1044 WP_218237647.1 type IV pilus twitching motility protein PilT Machinery gene
  KUD94_RS13235 (KUD94_13235) - 2748899..2749528 (+) 630 WP_218237648.1 cyclic nucleotide-binding domain-containing protein -
  KUD94_RS13240 (KUD94_13240) pilU 2749571..2750707 (+) 1137 WP_146912251.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KUD94_RS13245 (KUD94_13245) - 2750725..2751660 (+) 936 WP_218237649.1 NAD(P)-dependent oxidoreductase -
  KUD94_RS13250 (KUD94_13250) - 2751705..2752415 (-) 711 WP_218237650.1 BON domain-containing protein -
  KUD94_RS13255 (KUD94_13255) - 2752618..2753784 (-) 1167 WP_218237651.1 phospholipase A -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41757.20 Da        Isoelectric Point: 6.6113

>NTDB_id=586552 KUD94_RS13240 WP_146912251.1 2749571..2750707(+) (pilU) [Comamonas sp. NLF-1-9]
MERDQASKFINDLLKLMVSRGGSDLFITADFPPAIKVDGKMTKVSPQPLTAMHTLTLARSIMSDKQVADFERTKECNFAI
SPVGIGRFRVNAFIQQGNVGMVLRTIPLSLPSIDSLGLPQVLKEVAMTKRGLCIMVGATGSGKSTSLAAMVDWRNENSYG
HIVTVEDPIEFVHAHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVVFSETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRALVSQRLVPKQDGKGRIAAVEIMLNSPLIADFIFKGEVAEIKDVMKKSRNLG
MQTFDQALFDLFEANLISYEDALRNADSLNDLRLQIKLSSQRAKSGDLAAGTEQFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=586552 KUD94_RS13240 WP_146912251.1 2749571..2750707(+) (pilU) [Comamonas sp. NLF-1-9]
ATGGAACGCGACCAGGCAAGCAAATTCATCAACGACCTGCTCAAGCTGATGGTCAGCCGCGGTGGCAGCGACTTGTTCAT
CACCGCGGACTTCCCGCCGGCCATCAAGGTGGACGGCAAGATGACCAAGGTCTCGCCCCAGCCGCTCACGGCCATGCACA
CGCTCACCCTGGCGCGCTCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACCAAGGAATGCAACTTCGCCATC
TCGCCCGTGGGCATAGGGCGTTTTCGCGTCAATGCCTTCATCCAGCAGGGCAATGTCGGCATGGTGCTGCGCACCATCCC
GCTGAGTCTGCCGTCCATCGACAGCCTGGGCCTGCCCCAGGTGCTCAAGGAAGTGGCCATGACCAAGCGCGGCCTGTGCA
TCATGGTGGGTGCTACCGGCTCGGGCAAGTCCACGTCGCTCGCGGCCATGGTGGACTGGCGCAATGAAAATTCCTACGGC
CACATCGTGACCGTGGAAGACCCGATCGAGTTCGTGCACGCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTAGGCCT
GGATACCGACAGCTGGGAGGCGGCGCTGAAAAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAAACCATGGAGCATGCAGTGGTGTTTTCCGAGACCGGTCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAATTTCTTCCCCGAGGAGCGGCGCGCCCAGTTGCTCATGGACTTGTCGCTGAACCT
GCGCGCGCTGGTCTCGCAGCGCCTGGTGCCCAAGCAGGACGGCAAGGGGCGCATCGCGGCGGTGGAGATCATGCTCAATT
CGCCCCTGATCGCCGACTTCATCTTCAAGGGCGAGGTGGCCGAGATCAAGGACGTGATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGTTGTTCGACCTGTTCGAAGCCAACCTCATCAGCTACGAGGATGCACTGCGCAACGCCGA
TTCGCTCAACGACCTGCGCCTGCAGATCAAGCTCTCGAGCCAGCGTGCCAAGTCGGGCGACCTGGCAGCAGGCACGGAAC
AATTTGCCATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8RSY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.073

94.709

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Legionella pneumophila strain Lp02

44.745

88.095

0.394
  pilT Legionella pneumophila strain ERS1305867

44.745

88.095

0.394
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373