Detailed information    

experimental Experimentally validated

Overview

Name   recF   Type   Machinery gene
Locus tag   SPR_RS10345 Genome accession   NC_003098
Coordinates   2022400..2023497 (+) Length   365 a.a.
NCBI ID   WP_000266662.1    Uniprot ID   Q8DMX3
Organism   Streptococcus pneumoniae R6     
Function   homologous recombination   
Homologous recombination

Function

A ∼1.45-fold drop in transformation was observed with total chromosomal DNA in recFOR mutants. Transformation with chromosomal DNA stimulated unexpectedly high frequency (>30% of cells) formation of chromosome dimers as an intermediate in the generation of tandem duplications, and that RecFOR were crucial for dimer resolution. The site-specific recombinase XerS was also crucial for dimer resolution.


Genomic Context

Location: 2017400..2028497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS10320 (spr2027) pgsA 2017921..2018466 (-) 546 WP_000712132.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  SPR_RS10325 (spr2028) rodZ 2018477..2019298 (-) 822 WP_001227958.1 cytoskeleton protein RodZ -
  SPR_RS10330 (spr2029) yfmH 2019340..2020623 (-) 1284 WP_000170194.1 EF-P 5-aminopentanol modification-associated protein YfmH -
  SPR_RS10335 (spr2030) yfmF 2020620..2021870 (-) 1251 WP_000424263.1 EF-P 5-aminopentanol modification-associated protein YfmF -
  SPR_RS10340 (spr2031) yaaA 2022029..2022397 (+) 369 WP_000455903.1 S4 domain-containing protein YaaA -
  SPR_RS10345 (spr2032) recF 2022400..2023497 (+) 1098 WP_000266662.1 DNA replication/repair protein RecF Machinery gene
  SPR_RS10350 (spr2033) guaB 2023548..2025026 (-) 1479 WP_000073428.1 IMP dehydrogenase -
  SPR_RS10355 (spr2034) trpS 2025178..2026203 (-) 1026 WP_000165444.1 tryptophan--tRNA ligase -
  SPR_RS10360 (spr2035) - 2026409..2028031 (+) 1623 WP_000958770.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 41916.00 Da        Isoelectric Point: 9.0596

>NTDB_id=650 SPR_RS10345 WP_000266662.1 2022400..2023497(+) (recF) [Streptococcus pneumoniae R6]
MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIYFLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGS
IPLEIELTQKGRVTKVNHLKQARLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEELSISYQSSVNITDKQNLSESFKI
ALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFGSQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQ
LKLLETISQSIQTFITTTSLDHLQNLPENLSIFTIQDGKASVNGN

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=650 SPR_RS10345 WP_000266662.1 2022400..2023497(+) (recF) [Streptococcus pneumoniae R6]
ATGTGGCTACAACATCTATCTCTCAAGACTTTTCGTAATTACAAAGAAACGAAAATAGACTTTAATCCTAAATTGAATGT
CTTTTTGGGACGTAATGCACAAGGAAAAACAAACATGTTAGAGGCTATCTATTTTTTAGCCTTAACGCGTAGTCATCGAA
CTCGAACAGATAAAAATCTCATTCATTTTGATGAGGAACAACTTCATCTTTCAGGTCTCGTTCAGAAAAAAACTGGATCC
ATTCCCCTAGAAATCGAACTAACACAAAAAGGCCGTGTAACAAAAGTTAATCACTTAAAACAGGCACGCCTTTCAGATTA
TGTAGGACACATGAATGTTGTCTTATTTGCTCCTGAAGATTTACAACTAATTAAAGGAGCACCTTCGATTCGACGAAAAT
TCATTGATATGGAACTTGGGCAAATTAAGCCAATCTATTTATCTGACTTAACCAATTATAACCACATCCTAAAGCAAAGA
AACACTTACCTAAAATCAGCTCAAAAAATAGATGAAACATTCCTTTCTGTGTTAGATGATCAGCTAGTTGATTATGGATG
TCGTGTAATGAATCACCGCTTAGATTTCATAAAAAAACTAGAATCATTTGGGCGTAAGAAACATTTTGAACTCTCTAATC
AGATCGAAGAGTTGTCAATATCCTATCAATCTTCTGTCAATATAACTGACAAACAAAACTTATCCGAATCTTTCAAAATT
GCTTTAGAAAAAAGTAGATCCAGAGATTTATTTAAAAAGAATACTGGTGTCGGTCCTCATCGAGATGACATTTCTTTTTA
TATAAATGGGATGGATGCTAGTTTCGGAAGCCAAGGTCAACATCGTAGTCTCGTCCTCTCGATAAAATTAGCAGAAATCG
AATTAATGGAAAGCATTACCACAGAATCTCCTATATTACTGCTTGACGATGTGATGAGTGAACTTGACAACACTAGACAG
TTAAAATTATTAGAAACGATTTCTCAATCAATCCAAACCTTTATCACAACAACAAGCTTAGACCATCTTCAAAATCTGCC
AGAAAATCTAAGTATCTTCACTATTCAGGATGGTAAAGCTTCTGTAAATGGAAATTGA

Domains

Predicted by InterProScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(3-358)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DMX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

45.902

100

0.46

References

[1] Calum Johnston et al. (2015) RecFOR is not required for pneumococcal transformation but together with XerS for resolution of chromosome dimers frequently formed in the process. PLoS Genetics 11(1):e1004934. [PMID: 25569614]