Detailed information    

experimental Experimentally validated

Overview

Name   recF   Type   Machinery gene
Locus tag   BSU_00040 Genome accession   NC_000964
Coordinates   3437..4549 (+) Length   370 a.a.
NCBI ID   NP_387885.1    Uniprot ID   P05651
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   homologous recombination   
Homologous recombination

Function

The recF and addAB genes are not required for transformation with plasmid DNA, but have overlapping activities in transformation with chromosomal DNA.


Genomic Context

Location: 1..9549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_00010 (BSU00010) dnaA 410..1750 (+) 1341 NP_387882.1 chromosomal replication initiator informational ATPase -
  BSU_00020 (BSU00020) dnaN 1939..3075 (+) 1137 NP_387883.1 DNA polymerase III (beta subunit) -
  BSU_00030 (BSU00030) rlbA 3206..3421 (+) 216 NP_387884.1 RNA binding protein involved in ribosome maturation -
  BSU_00040 (BSU00040) recF 3437..4549 (+) 1113 NP_387885.1 RecA filament-DNA complex stabilisation, ssDNA and dsDNA binding, ATP binding Machinery gene
  BSU_00050 (BSU00050) remB 4567..4812 (+) 246 NP_387886.2 regulator of extracellular matrix formation -
  BSU_00060 (BSU00060) gyrB 4867..6783 (+) 1917 NP_387887.1 DNA gyrase (subunit B) -
  BSU_00070 (BSU00070) gyrA 6994..9459 (+) 2466 NP_387888.1 DNA gyrase (subunit A) -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42304.34 Da        Isoelectric Point: 7.3660

>NTDB_id=353 BSU_00040 NP_387885.1 3437..4549(+) (recF) [Bacillus subtilis subsp. subtilis str. 168]
MYIQNLELTSYRNYDHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLVEVAAKVVVKRLQFTAQLEKWAQPIHAGISRGLEELTLKYHTALDVSDPLDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=353 BSU_00040 NP_387885.1 3437..4549(+) (recF) [Bacillus subtilis subsp. subtilis str. 168]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTACGACCATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATCGGAGAAAACGCCCAGGGGAAGACAAACCTCATGGAGGCGATCTATGTCTTGTCCATGGCGAAATCGCACCGGA
CATCAAATGACAAAGAACTTATACGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATTGAACAGCAAAAGCTCAGCCAGTA
TGTCGGGGCCCTCAACACCATTATGTTCGCGCCGGAAGATTTAAATCTTGTAAAGGGAAGCCCTCAAGTGAGAAGGCGGT
TTCTTGACATGGAAATCGGACAGGTTTCTCCCGTCTACCTTCATGATCTTTCTCTTTACCAGAAAATCCTTTCCCAGCGG
AATCATTTTTTGAAACAGCTGCAAACAAGAAAACAAACTGACCGGACGATGCTCGATGTTCTGACCGATCAGCTTGTAGA
AGTTGCAGCAAAAGTCGTCGTAAAACGCCTGCAGTTTACAGCACAGCTCGAGAAATGGGCGCAGCCCATCCATGCAGGCA
TCTCAAGAGGGCTTGAAGAACTGACCCTGAAATACCATACAGCTCTTGATGTATCAGATCCCCTAGATTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATTGAGCGTGGTGTGACGCTGTCAGGGCCTCATCG
CGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGAACGACGGCGTTGT
CCCTTAAGCTGGCGGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTACTGAGT
GAACTGGATGATTATCGCCAGTCACACTTGCTTCATACGATCCAAGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGTCAAGCAGGAATGTTCCGTGTGCAAAATGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P05651

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] B J Haijema et al. (1996) Replacement of the lysine residue in the consensus ATP-binding sequence of the AddA subunit of AddAB drastically affects chromosomal recombination in transformation and transduction of Bacillus subtilis. Molecular Microbiology 21(5):989-99. [PMID: 8885269]