Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KUU75_RS02010 Genome accession   NZ_CP077997
Coordinates   431503..432525 (+) Length   340 a.a.
NCBI ID   WP_034052146.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SRRSH1521     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 426503..437525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUU75_RS01985 (KUU75_01985) - 426791..428758 (-) 1968 WP_010793544.1 dynamin-like GTPase family protein -
  KUU75_RS01990 (KUU75_01990) - 428866..429162 (-) 297 WP_031628111.1 DUF167 domain-containing protein -
  KUU75_RS01995 (KUU75_01995) - 429159..429752 (-) 594 WP_003084544.1 YggT family protein -
  KUU75_RS02000 (KUU75_02000) proC 429763..430584 (-) 822 WP_003084546.1 pyrroline-5-carboxylate reductase -
  KUU75_RS02005 (KUU75_02005) - 430596..431288 (-) 693 WP_010793543.1 YggS family pyridoxal phosphate-dependent enzyme -
  KUU75_RS02010 (KUU75_02010) pilT 431503..432525 (+) 1023 WP_034052146.1 type IV pilus twitching motility protein PilT Machinery gene
  KUU75_RS02015 (KUU75_02015) pilU 432703..433851 (+) 1149 WP_003100959.1 type IV pilus ATPase PilU Machinery gene
  KUU75_RS02020 (KUU75_02020) czcD 433859..434758 (-) 900 WP_003100957.1 CDF family zinc transporter CzcD -
  KUU75_RS02025 (KUU75_02025) - 434912..435316 (+) 405 WP_003084560.1 NINE protein -
  KUU75_RS02030 (KUU75_02030) - 435559..436932 (+) 1374 WP_003100955.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37593.39 Da        Isoelectric Point: 6.6660

>NTDB_id=585979 KUU75_RS02010 WP_034052146.1 431503..432525(+) (pilT) [Pseudomonas aeruginosa strain SRRSH1521]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGEVFKRVSDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRVVDVF
PAEEKAMVRSMLSESLQSVISQTLIKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCLKGLV
AKGLISRENAREKAKIPENF

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=585979 KUU75_RS02010 WP_034052146.1 431503..432525(+) (pilT) [Pseudomonas aeruginosa strain SRRSH1521]
ATGGATATTACCGAGCTGCTCGCCTTCAGTGCCAAACAGGGCGCTTCGGACCTGCACCTCTCCGCCGGCCTGCCACCCAT
GATCCGGGTGGATGGCGATGTACGCCGGATCAACCTGCCACCGCTGGAACACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTATTCCGGACCATTCCCTCCAAGGTACTGACCATGGA
GGAGCTTGGCATGGGAGAAGTGTTCAAACGTGTTTCAGACGTCCCGCGCGGGCTGGTACTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGACTACCTGAACAACACCAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCTCGGCTTCAGCGA
AGCGCTGCGGGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGGCCCTGACCGCGG
CGGAGACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCGAAGACCATCGACCGGGTGGTCGACGTGTTC
CCGGCCGAGGAAAAGGCCATGGTCCGCTCGATGCTCTCCGAGTCGCTGCAATCGGTGATCTCGCAGACCCTGATCAAGAA
GATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCCGCGAGGACAAGG
TCGCGCAGATGTATTCGGCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTCGACATGTGCCTCAAGGGCCTGGTC
GCCAAGGGCCTGATCAGCCGCGAGAACGCCCGCGAGAAGGCGAAGATCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

98.837

100

1
  pilT Pseudomonas stutzeri DSM 10701

93.023

100

0.941
  pilT Acinetobacter baumannii D1279779

83.14

100

0.841
  pilT Acinetobacter baumannii strain A118

83.14

100

0.841
  pilT Acinetobacter nosocomialis M2

82.849

100

0.838
  pilT Acinetobacter baylyi ADP1

80.523

100

0.815
  pilT Legionella pneumophila strain Lp02

73.373

99.412

0.729
  pilT Legionella pneumophila strain ERS1305867

73.373

99.412

0.729
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.668
  pilT Neisseria meningitidis 8013

65.507

100

0.665
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.588

100

0.656
  pilT Vibrio cholerae strain A1552

65.588

100

0.656
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.524
  pilU Vibrio cholerae strain A1552

40.708

99.706

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.597

98.529

0.4
  pilU Acinetobacter baylyi ADP1

39.017

100

0.397