Detailed information NTG0585980

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KUU75_RS02015
• Genome accession: NZ_CP077997
• Coordinates: 432703..433851 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain SRRSH1521
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 427703..438851

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KUU75_RS01990 (KUU75_01990)	-	428866..429162 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
KUU75_RS01995 (KUU75_01995)	-	429159..429752 (-)	594	WP_003084544.1	YggT family protein	-
KUU75_RS02000 (KUU75_02000)	proC	429763..430584 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
KUU75_RS02005 (KUU75_02005)	-	430596..431288 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
KUU75_RS02010 (KUU75_02010)	pilT	431503..432525 (+)	1023	WP_034052146.1	type IV pilus twitching motility protein PilT	Machinery gene
KUU75_RS02015 (KUU75_02015)	pilU	432703..433851 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
KUU75_RS02020 (KUU75_02020)	czcD	433859..434758 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
KUU75_RS02025 (KUU75_02025)	-	434912..435316 (+)	405	WP_003084560.1	NINE protein	-
KUU75_RS02030 (KUU75_02030)	-	435559..436932 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
KUU75_RS02035 (KUU75_02035)	-	436929..438113 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=585980 KUU75_RS02015 WP_003100959.1 432703..433851(+) (pilU) [Pseudomonas aeruginosa strain SRRSH1521]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=585980 KUU75_RS02015 WP_003100959.1 432703..433851(+) (pilU) [Pseudomonas aeruginosa strain SRRSH1521]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382