Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B1NLA3E_RS17350 Genome accession   NC_021171
Coordinates   3614327..3615367 (-) Length   346 a.a.
NCBI ID   WP_015595139.1    Uniprot ID   N0B4I6
Organism   Bacillus sp. 1NLA3E     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3609327..3620367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1NLA3E_RS17320 (B1NLA3E_16700) - 3609380..3610156 (-) 777 WP_015595133.1 pilus assembly protein PilO -
  B1NLA3E_RS17325 (B1NLA3E_16705) - 3610153..3610791 (-) 639 WP_015595134.1 DUF3185 family protein -
  B1NLA3E_RS17330 (B1NLA3E_16710) pilM 3610794..3611786 (-) 993 WP_015595135.1 pilus assembly protein PilM -
  B1NLA3E_RS17335 (B1NLA3E_16715) comC 3611804..3612553 (-) 750 WP_041580643.1 A24 family peptidase Machinery gene
  B1NLA3E_RS17340 (B1NLA3E_16720) - 3612579..3613007 (-) 429 WP_015595137.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  B1NLA3E_RS17345 (B1NLA3E_16725) - 3613114..3614325 (-) 1212 WP_015595138.1 type II secretion system F family protein -
  B1NLA3E_RS17350 (B1NLA3E_16730) pilT 3614327..3615367 (-) 1041 WP_015595139.1 type IV pilus twitching motility protein PilT Machinery gene
  B1NLA3E_RS17355 (B1NLA3E_16735) - 3615380..3617041 (-) 1662 WP_015595140.1 ATPase, T2SS/T4P/T4SS family -
  B1NLA3E_RS17360 (B1NLA3E_16740) - 3617067..3618386 (-) 1320 WP_041580644.1 VanW family protein -
  B1NLA3E_RS17365 (B1NLA3E_16745) - 3618446..3619738 (-) 1293 WP_015595142.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38650.57 Da        Isoelectric Point: 6.7784

>NTDB_id=58408 B1NLA3E_RS17350 WP_015595139.1 3614327..3615367(-) (pilT) [Bacillus sp. 1NLA3E]
MRERIDLLLRAAFELKASDIHLTVSVQPIFRIHGELKRYGKEVLKPEDTEAMARAITPDGKWGVFEEMGELDFSYSIPNV
SRYRVNIFKQRGCVALAIRTVPTRIPTIDELNLPDTLRRIVEKKQGLILVTGPTGSGKSTTLAAMINYMNQTMHEHIITL
EDPIEYLHKHGMSIIDQREIGFDTKNFGNALRAALRQDPDAILVGEMRDFETIQIAITAAETGHLVFGTLHTSSAPASVE
RMIDVFEPAQQPQVRTQLASVLLSIISQRLFKTADGKGRRAALEILINNAAIANLIRNDKIHQIESTMQTSRSQGMQTLE
MSIKELLETGVIAQEVADPYLKGPVN

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=58408 B1NLA3E_RS17350 WP_015595139.1 3614327..3615367(-) (pilT) [Bacillus sp. 1NLA3E]
GTGAGGGAAAGAATCGATCTATTATTACGAGCAGCCTTTGAGCTAAAGGCATCAGATATCCATTTAACAGTATCTGTACA
ACCCATTTTTCGAATCCATGGTGAATTGAAGCGCTATGGAAAAGAAGTTCTCAAGCCCGAGGATACCGAAGCGATGGCAA
GGGCGATTACCCCAGATGGAAAGTGGGGGGTATTTGAAGAAATGGGGGAGTTGGACTTTTCCTATTCGATTCCTAATGTA
TCACGGTACCGTGTCAATATATTCAAGCAAAGAGGCTGTGTGGCCTTGGCGATCCGGACGGTTCCGACTCGTATTCCGAC
TATTGATGAGTTAAATCTTCCTGATACGCTACGGAGAATTGTCGAGAAGAAACAAGGTTTAATCCTCGTGACAGGACCAA
CCGGAAGTGGTAAGTCAACGACGTTAGCGGCGATGATCAATTATATGAACCAAACGATGCACGAGCACATTATTACGCTA
GAGGACCCGATTGAATATTTGCACAAACATGGCATGTCAATTATCGACCAGCGCGAAATTGGCTTTGATACGAAGAACTT
TGGGAATGCCTTACGGGCAGCACTCCGCCAGGATCCAGATGCGATTCTAGTTGGAGAGATGCGTGACTTTGAAACGATTC
AAATTGCCATCACCGCTGCTGAAACAGGCCACTTAGTGTTTGGAACGCTCCATACATCTAGCGCACCTGCTTCAGTGGAA
CGAATGATTGATGTATTCGAGCCAGCCCAGCAGCCCCAAGTCCGTACCCAGCTTGCATCGGTATTACTATCGATTATTTC
ACAGCGACTGTTTAAAACGGCAGACGGAAAAGGCCGAAGAGCTGCACTAGAAATATTAATCAATAATGCAGCGATTGCAA
ACTTAATTCGAAATGATAAAATCCACCAAATTGAGTCGACCATGCAGACGTCTCGGTCACAAGGGATGCAAACGTTAGAA
ATGAGCATTAAAGAGTTGCTGGAAACGGGAGTCATTGCCCAAGAAGTTGCCGACCCATATTTAAAGGGACCGGTGAACTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N0B4I6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.453

95.665

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.453

95.665

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.701

96.532

0.48
  pilT Neisseria meningitidis 8013

49.699

95.954

0.477
  pilT Pseudomonas aeruginosa PAK

48.368

97.399

0.471
  pilT Neisseria gonorrhoeae MS11

49.096

95.954

0.471
  pilT Pseudomonas stutzeri DSM 10701

47.478

97.399

0.462
  pilT Acinetobacter baumannii D1279779

47.463

96.821

0.46
  pilT Acinetobacter nosocomialis M2

47.463

96.821

0.46
  pilT Acinetobacter baumannii strain A118

47.463

96.821

0.46
  pilT Acinetobacter baylyi ADP1

47.181

97.399

0.46
  pilT Legionella pneumophila strain Lp02

46.061

95.376

0.439
  pilT Legionella pneumophila strain ERS1305867

46.061

95.376

0.439
  pilU Pseudomonas stutzeri DSM 10701

41.212

95.376

0.393
  pilU Acinetobacter baylyi ADP1

39.706

98.266

0.39
  pilU Vibrio cholerae strain A1552

41.304

93.064

0.384

Multiple sequence alignment