Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6L40_RS05380 Genome accession   NZ_CP077401
Coordinates   1117913..1118947 (+) Length   344 a.a.
NCBI ID   WP_033114043.1    Uniprot ID   A0AAQ0EDN6
Organism   Aeromonas sp. FDAARGOS 1410     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1112913..1123947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L40_RS05350 (I6L40_05350) - 1113668..1114864 (+) 1197 WP_071913545.1 NupC/NupG family nucleoside CNT transporter -
  I6L40_RS05355 (I6L40_05355) - 1114967..1115386 (-) 420 WP_041209070.1 DUF4426 domain-containing protein -
  I6L40_RS05360 (I6L40_05360) yggU 1115416..1115715 (-) 300 WP_216995094.1 DUF167 family protein YggU -
  I6L40_RS05365 (I6L40_05365) - 1115715..1116266 (-) 552 WP_041209071.1 YggT family protein -
  I6L40_RS05370 (I6L40_05370) proC 1116278..1117105 (-) 828 WP_216995095.1 pyrroline-5-carboxylate reductase -
  I6L40_RS05375 (I6L40_05375) - 1117172..1117873 (-) 702 WP_071913549.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L40_RS05380 (I6L40_05380) pilT 1117913..1118947 (+) 1035 WP_033114043.1 type IVa pilus ATPase TapT Machinery gene
  I6L40_RS05385 (I6L40_05385) pilU 1118976..1120085 (+) 1110 WP_071913663.1 type IVa pilus ATPase TapU Machinery gene
  I6L40_RS05390 (I6L40_05390) yaaA 1120133..1120906 (+) 774 WP_071913551.1 peroxide stress protein YaaA -
  I6L40_RS05395 (I6L40_05395) - 1121101..1121529 (+) 429 WP_033114045.1 hypothetical protein -
  I6L40_RS05400 (I6L40_05400) - 1121844..1122335 (-) 492 WP_071913553.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  I6L40_RS05405 (I6L40_05405) arfB 1122357..1122773 (-) 417 WP_033114047.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  I6L40_RS05410 (I6L40_05410) glnK 1122975..1123313 (+) 339 WP_033114048.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38305.09 Da        Isoelectric Point: 6.7440

>NTDB_id=581249 I6L40_RS05380 WP_033114043.1 1117913..1118947(+) (pilT) [Aeromonas sp. FDAARGOS 1410]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGMVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=581249 I6L40_RS05380 WP_033114043.1 1117913..1118947(+) (pilT) [Aeromonas sp. FDAARGOS 1410]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCGTCGGATCTACACCTGTCCGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACATCGGGAAGTGCACGCCCTCATTTACGACA
TCATGAACGATCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCTTTCCAGCAAGCGCGCGGTTCCGGCGCGGTATTTCGTACCATCCCCAGCACAGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTTCGCAAGATCGCCGAGTTCCCGCGCGGTCTGGTGCTGGTGACCGGGCCGACCGGTT
CCGGTAAGTCGACCACGCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCATATCCTCACCATCGAAGAC
CCCATCGAATTTGTGCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCACCGCGATACCAAGAGCTTCAGCAA
TGCCCTGCGCTCGGCACTGCGGGAAGACCCTGACATCATTCTGGTAGGTGAGATGCGCGATCTTGAAACCATTCGCCTCG
CCATGACCGCAGCCGAAACCGGCCACCTTGTATTTGGCACCCTGCACACCTCGTCGGCGGCCAAAACCATCGACCGTATC
ATCGACGTCTTTCCCGGCGCTGAAAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGCGGCCGGGTGGCTGCTCACGAGATCATGATGGGGATCCCGGCGGTGCGTAATCTCATCC
GGGAGGACAAGATCGCCCAGCTCTACTCGGTGATACAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGTCTC
AAGCAGCTGGTCAGCCGTGGCATGGTGGCGTCTCTCGACGCCAAGGCCAAGGCGGTTGACCCCAACAGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.668

97.965

0.741
  pilT Vibrio cholerae strain A1552

75.668

97.965

0.741
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407