Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L40_RS05385 Genome accession   NZ_CP077401
Coordinates   1118976..1120085 (+) Length   369 a.a.
NCBI ID   WP_071913663.1    Uniprot ID   A0AAQ0J1U4
Organism   Aeromonas sp. FDAARGOS 1410     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1113976..1125085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L40_RS05355 (I6L40_05355) - 1114967..1115386 (-) 420 WP_041209070.1 DUF4426 domain-containing protein -
  I6L40_RS05360 (I6L40_05360) yggU 1115416..1115715 (-) 300 WP_216995094.1 DUF167 family protein YggU -
  I6L40_RS05365 (I6L40_05365) - 1115715..1116266 (-) 552 WP_041209071.1 YggT family protein -
  I6L40_RS05370 (I6L40_05370) proC 1116278..1117105 (-) 828 WP_216995095.1 pyrroline-5-carboxylate reductase -
  I6L40_RS05375 (I6L40_05375) - 1117172..1117873 (-) 702 WP_071913549.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L40_RS05380 (I6L40_05380) pilT 1117913..1118947 (+) 1035 WP_033114043.1 type IVa pilus ATPase TapT Machinery gene
  I6L40_RS05385 (I6L40_05385) pilU 1118976..1120085 (+) 1110 WP_071913663.1 type IVa pilus ATPase TapU Machinery gene
  I6L40_RS05390 (I6L40_05390) yaaA 1120133..1120906 (+) 774 WP_071913551.1 peroxide stress protein YaaA -
  I6L40_RS05395 (I6L40_05395) - 1121101..1121529 (+) 429 WP_033114045.1 hypothetical protein -
  I6L40_RS05400 (I6L40_05400) - 1121844..1122335 (-) 492 WP_071913553.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  I6L40_RS05405 (I6L40_05405) arfB 1122357..1122773 (-) 417 WP_033114047.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  I6L40_RS05410 (I6L40_05410) glnK 1122975..1123313 (+) 339 WP_033114048.1 P-II family nitrogen regulator -
  I6L40_RS05415 (I6L40_05415) - 1123507..1124514 (+) 1008 WP_216995096.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41084.10 Da        Isoelectric Point: 6.4422

>NTDB_id=581250 I6L40_RS05385 WP_071913663.1 1118976..1120085(+) (pilU) [Aeromonas sp. FDAARGOS 1410]
MNLDELLGQLVEKKGSDLFVTVGTPPTLKVNGHLLSLGGEALDKKAALTLVRETLSPEHFERYIRTREANYAIHRETLGR
FRVSAFWQQELPGMVVRRIETRIPTFDDLQLPVILQEVALAKRGLVLFVGATGAGKSTTQATMIGYRNQHADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSSETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLKAIVAQQLVPSVDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQS
LFNLFCSGQIGYSEALAHADSANDLRLLIKLSGRDQLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=581250 I6L40_RS05385 WP_071913663.1 1118976..1120085(+) (pilU) [Aeromonas sp. FDAARGOS 1410]
ATGAATCTGGATGAACTGCTGGGCCAGCTGGTAGAGAAAAAAGGGTCGGATCTCTTTGTGACGGTGGGGACGCCCCCCAC
CCTCAAGGTGAACGGCCATCTGCTCTCTTTGGGAGGCGAAGCGCTCGATAAAAAGGCGGCGTTGACGCTGGTGAGGGAGA
CCCTGAGCCCCGAGCATTTCGAGCGCTATATCCGCACCAGGGAGGCCAACTACGCCATTCACCGCGAAACTTTGGGCCGG
TTTCGGGTCAGCGCTTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTTAGGCGTATCGAGACCCGCATCCCGACCTTCGA
TGACCTGCAACTGCCCGTGATCCTGCAGGAGGTCGCATTGGCCAAGCGCGGACTGGTGCTGTTTGTTGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCGACCATGATTGGTTATCGCAACCAGCATGCCGACGGCCATATTCTGACGGTGGAGGAT
CCGGTGGAGTTTGTCCATCAGCATGGCCGCAGCCTGATAACCCAGCGGGAGGTGGGGATCGATACCGAGTCGTTCGATGT
GGCGTTGAAAAGCTCGCTGCGTCAGGCACCTGACGTCATTTTGATCGGCGAGATCAGAAGCTCTGAGACCATGGAGTTTG
CCCTGCAATTCGCCGAGACCGGTCACCTCTGCCTCGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATT
CTGCATCTGGTGCCGCAGGAGAAGCATCGTCAGTTCCTGTTCGATCTCTCCTTCAACCTCAAGGCCATTGTCGCCCAGCA
GCTGGTGCCGAGTGTGGATGGCAAGCGGCGCTGCGCCGCATTCGAGATCCTGCTCAACACCCCGCTTATCACCGACATCA
TTCGCAAAGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAATCGACCGAGCTTGGCATGCAAACCTTCGATCAATCG
CTTTTCAACCTGTTCTGCTCCGGCCAGATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGGCT
GCTGATCAAGCTTTCCGGCCGTGATCAGCTTGGAACCGGTACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.054

99.729

0.599
  pilU Pseudomonas stutzeri DSM 10701

60.114

95.122

0.572
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.708

91.87

0.374
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363