Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6L25_RS11260 Genome accession   NZ_CP077398
Coordinates   2423449..2424486 (+) Length   345 a.a.
NCBI ID   WP_006581387.1    Uniprot ID   A0A0A7XL61
Organism   Acinetobacter nosocomialis strain FDAARGOS 1395     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2418449..2429486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L25_RS11250 (I6L25_11250) - 2421680..2422522 (-) 843 WP_004710225.1 undecaprenyl-diphosphate phosphatase -
  I6L25_RS11255 (I6L25_11255) - 2422630..2423322 (-) 693 WP_004710226.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L25_RS11260 (I6L25_11260) pilT 2423449..2424486 (+) 1038 WP_006581387.1 type IV pilus twitching motility protein PilT Machinery gene
  I6L25_RS11265 (I6L25_11265) pilU 2424514..2425632 (+) 1119 WP_002052019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6L25_RS11270 (I6L25_11270) fur 2425702..2426139 (-) 438 WP_002052029.1 ferric iron uptake transcriptional regulator -
  I6L25_RS11275 (I6L25_11275) - 2426251..2426649 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  I6L25_RS11280 (I6L25_11280) - 2426705..2427028 (-) 324 WP_002052062.1 RnfH family protein -
  I6L25_RS11285 (I6L25_11285) - 2427028..2428095 (-) 1068 WP_004710227.1 hypothetical protein -
  I6L25_RS11290 (I6L25_11290) - 2428248..2428703 (-) 456 WP_004710228.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38328.92 Da        Isoelectric Point: 6.7271

>NTDB_id=581171 I6L25_RS11260 WP_006581387.1 2423449..2424486(+) (pilT) [Acinetobacter nosocomialis strain FDAARGOS 1395]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=581171 I6L25_RS11260 WP_006581387.1 2423449..2424486(+) (pilT) [Acinetobacter nosocomialis strain FDAARGOS 1395]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCATCCGATTTGCACTTATCAGCTGGTATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATTAACTTGCCAGCCTTAGAACATAAAGATGTCCACCGCCTCGTTTATGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCGAATGTTGCGCGT
TTCCGTGTAAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGATATTTGCGACTATCCTCGCGGGATTGTACTGGTCACAGGTCCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCAATGCTCGATTATATTAATGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACATCAGTCCAAGAAATGTCTGATTAACCAACGTGAAGTGCATCGTGATACACATGGCTTTAATGA
AGCCCTTCGCTCAGCACTACGTGAAGACCCAGATATTATTTTAGTCGGTGAGATGCGTGACCTTGAAACGATTCGATTAG
CACTTACTGCTGCCGAAACAGGTCACTTAGTATTTGGTACGCTTCACACCACATCGGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCCGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGTCGTGTTGCGGCACATGAAATTATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTATTCAGCTATTCAGACTGGTGCTAACCATGGTATGACAACCCTCGACCAAAGCCTA
AAAGGTTTGGTTTCACGTGGTGTAATTAGTCCACAAACAGCCCGTACAGCGGCGAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A7XL61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

100

100

1
  pilT Acinetobacter baumannii D1279779

99.71

100

0.997
  pilT Acinetobacter baumannii strain A118

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388