Detailed information NTG0581172

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6L25_RS11265
• Genome accession: NZ_CP077398
• Coordinates: 2424514..2425632 (+)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain FDAARGOS 1395
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2419514..2430632

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6L25_RS11250 (I6L25_11250)	-	2421680..2422522 (-)	843	WP_004710225.1	undecaprenyl-diphosphate phosphatase	-
I6L25_RS11255 (I6L25_11255)	-	2422630..2423322 (-)	693	WP_004710226.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6L25_RS11260 (I6L25_11260)	pilT	2423449..2424486 (+)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
I6L25_RS11265 (I6L25_11265)	pilU	2424514..2425632 (+)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6L25_RS11270 (I6L25_11270)	fur	2425702..2426139 (-)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
I6L25_RS11275 (I6L25_11275)	-	2426251..2426649 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
I6L25_RS11280 (I6L25_11280)	-	2426705..2427028 (-)	324	WP_002052062.1	RnfH family protein	-
I6L25_RS11285 (I6L25_11285)	-	2427028..2428095 (-)	1068	WP_004710227.1	hypothetical protein	-
I6L25_RS11290 (I6L25_11290)	-	2428248..2428703 (-)	456	WP_004710228.1	bacteriohemerythrin	-
I6L25_RS11295 (I6L25_11295)	-	2428956..2429804 (-)	849	WP_009505210.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=581172 I6L25_RS11265 WP_002052019.1 2424514..2425632(+) (pilU) [Acinetobacter nosocomialis strain FDAARGOS 1395]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=581172 I6L25_RS11265 WP_002052019.1 2424514..2425632(+) (pilU) [Acinetobacter nosocomialis strain FDAARGOS 1395]
ATGGATTTTAATGACTTGCTCAATCTGATGGTTGAAAAAAAATCATCCGATCTGTTTATTACAGATGGTGTTGCACCATC
AATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTAATTGGTCAACTCTTACATTCCA
TCATGAGTGAAAAACAGCGTAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGCATTATTACTCAACGTGAAGTCGGTATTGATACGGACTCATTCGA
AATTGCGTTAAAAAACACGTTACGACAAGCGCCAGATGTGATCTTAATTGGTGAAATCCGCTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGACACCTTGTATTAGCTACAATGCATGCCAACAATGCCAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
ACAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTCGACTTATACAAAGCCGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAACTTGCAAATGAAGGTCCTGATCAAATAACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403