Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6L45_RS03670 Genome accession   NZ_CP077363
Coordinates   770407..771441 (+) Length   344 a.a.
NCBI ID   WP_216983475.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1415     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 765407..776441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L45_RS03630 (I6L45_03630) - 765568..765942 (+) 375 WP_216983471.1 YacL family protein -
  I6L45_RS03635 (I6L45_03635) - 765995..766288 (+) 294 WP_043757565.1 hypothetical protein -
  I6L45_RS03640 (I6L45_03640) - 766487..767318 (+) 832 Protein_694 lysostaphin resistance A-like protein -
  I6L45_RS03645 (I6L45_03645) - 767391..767810 (-) 420 WP_043757560.1 DUF4426 domain-containing protein -
  I6L45_RS03650 (I6L45_03650) yggU 767815..768123 (-) 309 WP_043809026.1 DUF167 family protein YggU -
  I6L45_RS03655 (I6L45_03655) - 768123..768674 (-) 552 WP_216983472.1 YggT family protein -
  I6L45_RS03660 (I6L45_03660) proC 768699..769523 (-) 825 WP_216983473.1 pyrroline-5-carboxylate reductase -
  I6L45_RS03665 (I6L45_03665) - 769660..770367 (-) 708 WP_216983474.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L45_RS03670 (I6L45_03670) pilT 770407..771441 (+) 1035 WP_216983475.1 type IVa pilus ATPase TapT Machinery gene
  I6L45_RS03675 (I6L45_03675) pilU 771463..772572 (+) 1110 WP_216983476.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6L45_RS03680 (I6L45_03680) yaaA 772606..773379 (+) 774 WP_216983477.1 peroxide stress protein YaaA -
  I6L45_RS03685 (I6L45_03685) srmB 773564..774784 (-) 1221 WP_134694885.1 ATP-dependent RNA helicase SrmB -
  I6L45_RS03690 (I6L45_03690) - 774912..775622 (+) 711 WP_216983478.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38261.08 Da        Isoelectric Point: 6.8744

>NTDB_id=580884 I6L45_RS03670 WP_216983475.1 770407..771441(+) (pilT) [Aeromonas sp. FDAARGOS 1415]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHRKVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLDDLEAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGIVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=580884 I6L45_RS03670 WP_216983475.1 770407..771441(+) (pilT) [Aeromonas sp. FDAARGOS 1415]
ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCCGCCGGGGTTCCTCCGAT
GATCAGGGTTGACGGAGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGAAAGTTCACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAGGTGCCCGGCATGGCGCGT
TTCCGGGTCAACGCCTTCCAGCAGGCCCGCGGTTCGGGCGCCGTGTTTCGGACCATCCCGAGCACTGTCCTGACCCTGGA
CGATCTGGAGGCACCGGAGATATTCCGCAAGATAGCGGAATTCCCCCGCGGCCTGGTGCTGGTGACCGGGCCAACCGGCT
CGGGCAAATCCACCACTCTGGCGGCCATGGTCGATTACATCAACGACAACTTCCACCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAACGCTGCCTAGTCAACCAGCGGGAGGTGCATCGCGACACCAAGAGCTTCAGCAA
TGCCCTGCGCTCGGCCCTGCGTGAAGACCCGGACATCATACTGGTGGGGGAAATGCGCGACCTCGAGACCATCCGTCTCG
CCATGACAGCGGCCGAGACCGGTCATCTGGTGTTTGGCACCCTGCATACCTCCTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTTCCGGGATCCGAGAAAGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAACGGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATCCCTGCGGTGCGAAACCTCATCC
GTGAAGACAAGGTGGCCCAGCTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAACAGCTGGTCAATCGGGGCATAGTCGCCGCCCTCGATGCCAAGGCCAAGGCAGTCGATCCCAACACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.006

99.128

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

76.163

100

0.762
  pilT Acinetobacter baumannii strain A118

76.163

100

0.762
  pilT Acinetobacter nosocomialis M2

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

76.54

99.128

0.759
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.668

97.965

0.741
  pilT Vibrio cholerae strain A1552

75.668

97.965

0.741
  pilT Legionella pneumophila strain Lp02

72.059

98.837

0.712
  pilT Legionella pneumophila strain ERS1305867

72.059

98.837

0.712
  pilT Neisseria meningitidis 8013

68.732

98.547

0.677
  pilT Neisseria gonorrhoeae MS11

68.437

98.547

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.339

99.419

0.52
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407