Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L45_RS03675 Genome accession   NZ_CP077363
Coordinates   771463..772572 (+) Length   369 a.a.
NCBI ID   WP_216983476.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1415     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 766463..777572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L45_RS03640 (I6L45_03640) - 766487..767318 (+) 832 Protein_694 lysostaphin resistance A-like protein -
  I6L45_RS03645 (I6L45_03645) - 767391..767810 (-) 420 WP_043757560.1 DUF4426 domain-containing protein -
  I6L45_RS03650 (I6L45_03650) yggU 767815..768123 (-) 309 WP_043809026.1 DUF167 family protein YggU -
  I6L45_RS03655 (I6L45_03655) - 768123..768674 (-) 552 WP_216983472.1 YggT family protein -
  I6L45_RS03660 (I6L45_03660) proC 768699..769523 (-) 825 WP_216983473.1 pyrroline-5-carboxylate reductase -
  I6L45_RS03665 (I6L45_03665) - 769660..770367 (-) 708 WP_216983474.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L45_RS03670 (I6L45_03670) pilT 770407..771441 (+) 1035 WP_216983475.1 type IVa pilus ATPase TapT Machinery gene
  I6L45_RS03675 (I6L45_03675) pilU 771463..772572 (+) 1110 WP_216983476.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6L45_RS03680 (I6L45_03680) yaaA 772606..773379 (+) 774 WP_216983477.1 peroxide stress protein YaaA -
  I6L45_RS03685 (I6L45_03685) srmB 773564..774784 (-) 1221 WP_134694885.1 ATP-dependent RNA helicase SrmB -
  I6L45_RS03690 (I6L45_03690) - 774912..775622 (+) 711 WP_216983478.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  I6L45_RS03695 (I6L45_03695) brnQ 775858..777120 (+) 1263 WP_043757542.1 branched-chain amino acid transport system II carrier protein -
  I6L45_RS03700 (I6L45_03700) - 777227..777454 (-) 228 WP_216983479.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41163.34 Da        Isoelectric Point: 6.1309

>NTDB_id=580885 I6L45_RS03675 WP_216983476.1 771463..772572(+) (pilU) [Aeromonas sp. FDAARGOS 1415]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRFIPLGDKPLDREAALTLVRESLDQAHFERYTRTREANCAIQRAALGR
FRVSAFWQQDMPGMVLRRIETKIPTFDELMLPPILQDVVMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHGEGHILTVED
PVEFVHQHDRCLVTQREVGLDTESFDTALKNSLRQAPDVILLGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPATHGQRRVAAFEILLNTPLITDIIRKGEMQRLKEVMTKSSELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=580885 I6L45_RS03675 WP_216983476.1 771463..772572(+) (pilU) [Aeromonas sp. FDAARGOS 1415]
ATGAACATGGATGTTCTGCTCGCAGCACTGGTTGAACAGAAGGGCTCGGATCTCTTCATCACCGTCGATGCCCCTCCCAC
CCTCAAGGTGAACGGGCGCTTCATCCCCCTCGGCGATAAACCGCTCGACAGGGAAGCAGCCCTGACCCTGGTCAGGGAGA
GCCTGGATCAGGCCCACTTTGAACGCTATACCCGCACCCGGGAGGCCAACTGCGCCATTCAGCGGGCAGCCCTCGGCCGG
TTCCGGGTCAGCGCCTTCTGGCAGCAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCAAGATCCCCACCTTTGA
CGAGCTGATGCTGCCCCCCATATTGCAGGACGTCGTCATGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGCGCCACGGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGTTACCGCAACCAGCATGGTGAGGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGCCTGGTGACCCAGCGGGAGGTGGGACTAGATACAGAGTCCTTCGACAC
CGCACTCAAGAACTCACTACGCCAGGCGCCGGACGTCATTCTCCTCGGCGAGATCCGCTCCCAGGAAACCATGGAGTTCG
CCATCCAGTTCGCCGAGACCGGTCACCTCTGCCTCGCCACCCTGCACGCCAACAACGCCAACCAGGCACTGGATCGCATT
CTCCACCTGGTGCCGCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCACAGCA
GCTGCTGCCCGCCACCCATGGGCAGCGCCGTGTCGCCGCCTTCGAGATCCTGCTCAACACGCCTCTCATCACCGACATCA
TCCGCAAGGGAGAGATGCAGCGGCTCAAGGAGGTGATGACCAAATCCAGCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTCTTCACCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCTCATGCCGACTCCGCCAACGACCTGCGGCT
GCTCATCAAGCTGTCCGGTCGCGAGCAACTGGGAGCCGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.239

99.729

0.591
  pilU Pseudomonas stutzeri DSM 10701

58.405

95.122

0.556
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36