Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KNV97_RS16680 Genome accession   NZ_CP076643
Coordinates   2314197..2315234 (-) Length   345 a.a.
NCBI ID   WP_136487563.1    Uniprot ID   -
Organism   Vibrio ostreae strain OG9-811     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2309197..2320234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KNV97_RS16655 (KNV97_16655) rsmE 2310098..2310829 (+) 732 WP_218562396.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KNV97_RS16660 (KNV97_16660) gshB 2310841..2311797 (+) 957 WP_136487560.1 glutathione synthase -
  KNV97_RS16665 (KNV97_16665) - 2311875..2312439 (+) 565 Protein_2062 YqgE/AlgH family protein -
  KNV97_RS16670 (KNV97_16670) ruvX 2312468..2312893 (+) 426 WP_136487562.1 Holliday junction resolvase RuvX -
  KNV97_RS16675 (KNV97_16675) pilU 2313036..2314151 (-) 1116 WP_218563448.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KNV97_RS16680 (KNV97_16680) pilT 2314197..2315234 (-) 1038 WP_136487563.1 type IV pilus twitching motility protein PilT Machinery gene
  KNV97_RS16685 (KNV97_16685) - 2315275..2315985 (+) 711 WP_136487564.1 YggS family pyridoxal phosphate-dependent enzyme -
  KNV97_RS16690 (KNV97_16690) proC 2316197..2317015 (+) 819 WP_218562397.1 pyrroline-5-carboxylate reductase -
  KNV97_RS16695 (KNV97_16695) - 2317138..2317695 (+) 558 WP_136487566.1 YggT family protein -
  KNV97_RS16700 (KNV97_16700) yggU 2317695..2317985 (+) 291 WP_136487567.1 DUF167 family protein YggU -
  KNV97_RS16705 (KNV97_16705) - 2318154..2318555 (+) 402 WP_256612999.1 DUF4426 domain-containing protein -
  KNV97_RS16710 (KNV97_16710) - 2318573..2319196 (+) 624 WP_218562399.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38100.65 Da        Isoelectric Point: 6.6687

>NTDB_id=576825 KNV97_RS16680 WP_136487563.1 2314197..2315234(-) (pilT) [Vibrio ostreae strain OG9-811]
MDISELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHADVHRLIFEIMNDAQRSEYEERLEVDFSFELPSVGR
FRVNAFHQARGASAVLRTIPTTIPSLEQLEAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGNDKSMVRSMLSESLRAVIAQKLLKRVGGGRVACHEVMLATPAIRNLIREDKVAQMYSIIQTGAMHGMQTMEQNA
KQLLAKGLVDQAEVQQKIATDIAGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=576825 KNV97_RS16680 WP_136487563.1 2314197..2315234(-) (pilT) [Vibrio ostreae strain OG9-811]
ATGGATATCTCTGAATTACTGGAATTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCTGCAGGCGTTCCTCCGAT
GGTTCGCATCGACGGCGAGGTGAGAAAGCTTGGTGTGCCTGCTTTTAGCCATGCTGACGTGCATCGATTAATTTTTGAAA
TTATGAACGATGCCCAGCGCAGCGAGTATGAAGAACGGCTCGAAGTGGACTTTTCCTTTGAACTGCCGTCGGTCGGCCGT
TTCCGGGTCAACGCATTTCATCAGGCGCGTGGTGCCTCAGCGGTGCTGCGGACCATCCCGACCACGATTCCAAGTTTAGA
GCAGCTGGAAGCGCCGGAGATCTTCAGTAAAATCGCCAACTATGAGAAAGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CCGGTAAATCGACCACACTGGCGGCGATGGTGGATTACATCAATGCCCACCATAATAAACATATCCTGACCATTGAAGAC
CCGATCGAGTTTGTCCATAGCAACAATAAATGCCTGATTAACCAGCGTGAAGTGCACCGCGATACCCATAGCTTTAAAAA
TGCGCTGCGCAGCGCGCTGCGTGAGGATCCGGATGTGATTCTGGTTGGTGAGCTGCGTGACCAGGAGACTATCAGCCTGG
CGCTGACCGCCGCAGAAACCGGCCACTTAGTGTTTGGTACCCTGCACACCAGCTCGGCGGCGAAAACCATCGACCGGATT
ATCGATGTCTTCCCCGGCAATGATAAATCTATGGTGCGCTCCATGCTGTCGGAGTCACTGCGCGCGGTGATCGCCCAGAA
GCTGCTCAAACGGGTCGGGGGCGGCCGGGTGGCCTGTCATGAGGTGATGCTGGCCACACCGGCGATCCGTAACTTGATCC
GCGAGGATAAAGTGGCGCAGATGTACTCGATTATCCAGACCGGTGCCATGCACGGCATGCAGACCATGGAGCAGAATGCC
AAGCAGCTGCTGGCGAAAGGGCTGGTGGATCAGGCTGAAGTGCAGCAAAAAATCGCCACCGATATTGCTGGGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

92.174

100

0.922
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.174

100

0.922
  pilT Acinetobacter baylyi ADP1

71.818

95.652

0.687
  pilT Acinetobacter baumannii D1279779

71.429

95.362

0.681
  pilT Acinetobacter baumannii strain A118

71.429

95.362

0.681
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Acinetobacter nosocomialis M2

71.125

95.362

0.678
  pilT Pseudomonas aeruginosa PAK

67.656

97.681

0.661
  pilT Legionella pneumophila strain Lp02

67.372

95.942

0.646
  pilT Legionella pneumophila strain ERS1305867

67.372

95.942

0.646
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.515

95.652

0.493
  pilU Vibrio cholerae strain A1552

44

94.203

0.414
  pilU Pseudomonas stutzeri DSM 10701

41.768

95.072

0.397
  pilU Acinetobacter baylyi ADP1

41.25

92.754

0.383