Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KNV97_RS16675 Genome accession   NZ_CP076643
Coordinates   2313036..2314151 (-) Length   371 a.a.
NCBI ID   WP_218563448.1    Uniprot ID   A0A975YQ07
Organism   Vibrio ostreae strain OG9-811     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2308036..2319151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KNV97_RS16645 (KNV97_16645) - 2308476..2308976 (+) 501 WP_136487557.1 SprT family zinc-dependent metalloprotease -
  KNV97_RS16650 (KNV97_16650) - 2309158..2309873 (+) 716 Protein_2059 endonuclease -
  KNV97_RS16655 (KNV97_16655) rsmE 2310098..2310829 (+) 732 WP_218562396.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KNV97_RS16660 (KNV97_16660) gshB 2310841..2311797 (+) 957 WP_136487560.1 glutathione synthase -
  KNV97_RS16665 (KNV97_16665) - 2311875..2312439 (+) 565 Protein_2062 YqgE/AlgH family protein -
  KNV97_RS16670 (KNV97_16670) ruvX 2312468..2312893 (+) 426 WP_136487562.1 Holliday junction resolvase RuvX -
  KNV97_RS16675 (KNV97_16675) pilU 2313036..2314151 (-) 1116 WP_218563448.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KNV97_RS16680 (KNV97_16680) pilT 2314197..2315234 (-) 1038 WP_136487563.1 type IV pilus twitching motility protein PilT Machinery gene
  KNV97_RS16685 (KNV97_16685) - 2315275..2315985 (+) 711 WP_136487564.1 YggS family pyridoxal phosphate-dependent enzyme -
  KNV97_RS16690 (KNV97_16690) proC 2316197..2317015 (+) 819 WP_218562397.1 pyrroline-5-carboxylate reductase -
  KNV97_RS16695 (KNV97_16695) - 2317138..2317695 (+) 558 WP_136487566.1 YggT family protein -
  KNV97_RS16700 (KNV97_16700) yggU 2317695..2317985 (+) 291 WP_136487567.1 DUF167 family protein YggU -
  KNV97_RS16705 (KNV97_16705) - 2318154..2318555 (+) 402 WP_256612999.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40869.44 Da        Isoelectric Point: 5.2856

>NTDB_id=576824 KNV97_RS16675 WP_218563448.1 2313036..2314151(-) (pilU) [Vibrio ostreae strain OG9-811]
MQLDECLQGMVTRKASDIYITVGAPLLYRVNGALEAEGSALGSAQVGQLLDAMMDSARQQEFTASHEANFALVRDFGRFR
VSAFMQREQPGAVIRRIETDIPTFEQLDLPPVLKDLALAKRGLVLVVGATGSGKSTTLAAMTGYRNAERSGHILSVEDPI
EFVHPHQRSIVTQREVGLDTDSFEVALKNSLRQAPDMIVIGEIRTRETMEYAMTFAETGHLCLATLHANNANQALERILH
LVSKERREQFLFDLSLNLQGIIAQQLLADKQGQGRHGVFEILLNSPRVADLIRRGDLHELKATMAKSRENGMQTFDQALY
QLVAEDKIDEQEALHSADSANDLRLMLKTRSGTEGSALSGGSLANVQIDMD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=576824 KNV97_RS16675 WP_218563448.1 2313036..2314151(-) (pilU) [Vibrio ostreae strain OG9-811]
ATGCAACTCGATGAGTGCCTGCAGGGCATGGTGACGCGTAAAGCGTCCGATATCTATATCACGGTCGGCGCGCCGCTGCT
GTACCGGGTCAACGGCGCACTGGAGGCTGAGGGCAGTGCACTTGGCTCGGCTCAGGTCGGGCAACTGCTGGATGCGATGA
TGGACAGTGCCCGCCAGCAAGAGTTTACCGCCAGCCATGAAGCTAATTTTGCCCTGGTGCGTGACTTTGGCCGTTTCCGG
GTCAGTGCTTTTATGCAGCGCGAGCAGCCGGGCGCGGTGATCCGCCGCATCGAAACCGACATCCCGACCTTTGAGCAGCT
CGATTTGCCGCCGGTACTGAAAGATCTGGCACTGGCCAAGCGCGGCCTGGTGCTGGTAGTGGGGGCGACCGGCTCCGGTA
AGTCGACCACCCTGGCGGCGATGACCGGCTATCGCAATGCCGAGCGCAGCGGGCATATCCTCAGCGTGGAAGATCCGATT
GAGTTCGTTCATCCCCATCAGCGCAGTATCGTGACCCAGCGTGAAGTCGGCCTGGATACCGACAGTTTTGAAGTGGCGCT
GAAAAACTCGCTGCGTCAGGCGCCGGACATGATCGTCATCGGGGAGATCCGCACACGTGAGACGATGGAATACGCCATGA
CCTTTGCCGAAACCGGCCATCTGTGTCTGGCTACCCTGCACGCTAATAATGCCAACCAGGCGCTGGAGCGCATTTTACAT
CTGGTCAGCAAAGAGCGCCGCGAGCAGTTTCTGTTTGATCTGTCGCTCAACCTGCAAGGGATTATCGCGCAGCAATTGCT
GGCGGATAAACAGGGTCAGGGGCGGCACGGGGTGTTTGAAATATTGCTCAACAGTCCGCGCGTGGCGGATCTTATCCGGC
GCGGCGATCTGCATGAACTGAAAGCGACCATGGCCAAATCACGTGAAAACGGCATGCAGACCTTTGATCAGGCGCTGTAC
CAGCTGGTGGCAGAGGATAAAATCGATGAGCAGGAAGCGCTGCACAGTGCCGATTCGGCCAATGATCTGCGTCTGATGCT
CAAAACCCGCTCGGGCACAGAGGGTTCTGCGCTGTCCGGCGGTTCACTGGCGAATGTGCAGATTGATATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

78.167

100

0.782
  pilU Pseudomonas stutzeri DSM 10701

58.242

98.113

0.571
  pilU Acinetobacter baylyi ADP1

52.422

94.609

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.884

97.574

0.399
  pilT Legionella pneumophila strain Lp02

40.708

91.375

0.372
  pilT Legionella pneumophila strain ERS1305867

40.708

91.375

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.027

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.027

0.369
  pilT Acinetobacter baumannii D1279779

39.823

91.375

0.364
  pilT Acinetobacter baumannii strain A118

39.823

91.375

0.364
  pilT Acinetobacter nosocomialis M2

39.528

91.375

0.361