Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TOL_RS17030 Genome accession   NC_020888
Coordinates   3700365..3701399 (-) Length   344 a.a.
NCBI ID   WP_015488611.1    Uniprot ID   M5E9B4
Organism   Thalassolituus oleivorans MIL-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3695365..3706399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL_RS16995 (TOL_3519) moaA 3695852..3696826 (+) 975 WP_015488604.1 GTP 3',8-cyclase MoaA -
  TOL_RS17000 (TOL_3520) mobA 3696828..3697421 (+) 594 WP_015488605.1 molybdenum cofactor guanylyltransferase -
  TOL_RS17005 (TOL_3521) moaB 3697423..3697965 (+) 543 WP_015488606.1 molybdenum cofactor biosynthesis protein B -
  TOL_RS17010 (TOL_3522) moaC 3697972..3698460 (+) 489 WP_015488607.1 cyclic pyranopterin monophosphate synthase MoaC -
  TOL_RS17015 (TOL_3523) - 3698457..3698705 (+) 249 WP_015488608.1 MoaD/ThiS family protein -
  TOL_RS17020 (TOL_3524) moaE 3698707..3699162 (+) 456 WP_015488609.1 molybdopterin synthase catalytic subunit MoaE -
  TOL_RS17025 (TOL_3525) pilU 3699237..3700352 (-) 1116 WP_015488610.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TOL_RS17030 (TOL_3526) pilT 3700365..3701399 (-) 1035 WP_015488611.1 type IV pilus twitching motility protein PilT Machinery gene
  TOL_RS17035 (TOL_3527) - 3701559..3702245 (+) 687 WP_015488612.1 YggS family pyridoxal phosphate-dependent enzyme -
  TOL_RS17040 (TOL_3528) proC 3702310..3703128 (+) 819 WP_015488613.1 pyrroline-5-carboxylate reductase -
  TOL_RS17045 (TOL_3529) - 3703225..3703812 (+) 588 WP_015488614.1 YggT family protein -
  TOL_RS17050 (TOL_3530) - 3703816..3704127 (+) 312 WP_015488615.1 DUF167 family protein -
  TOL_RS17055 (TOL_3531) metX 3704185..3705351 (+) 1167 WP_015488616.1 homoserine O-succinyltransferase MetX -
  TOL_RS17060 (TOL_3532) metW 3705344..3705946 (+) 603 WP_015488617.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38019.55 Da        Isoelectric Point: 6.5073

>NTDB_id=57645 TOL_RS17030 WP_015488611.1 3700365..3701399(-) (pilT) [Thalassolituus oleivorans MIL-1]
MDITELLAFSAKQGASDLHLSADLPPMIRVDGDVRRINLPPLGHKEVHALVYDIMNDKQRKDFEEFLETDFSFEVSGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMDQLGMGQVFKDMANISRGIVLVTGPTGSGKSTTLAAMVDYINETKYNHILTVED
PIEFVHESKKSLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHVVFGTLHTSSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQGVVSQGLLKKNGGGRIAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGASYGMQTMDQCL
QNMVGKGLISRETAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=57645 TOL_RS17030 WP_015488611.1 3700365..3701399(-) (pilT) [Thalassolituus oleivorans MIL-1]
ATGGATATTACCGAACTATTGGCATTCAGCGCGAAGCAAGGCGCATCCGATTTACATCTTTCAGCTGATTTACCGCCGAT
GATTCGTGTAGACGGCGATGTGCGTCGAATTAATTTGCCGCCGTTGGGTCACAAAGAAGTACACGCGCTGGTTTACGATA
TTATGAACGATAAGCAGCGTAAGGATTTTGAAGAGTTCTTAGAAACTGACTTTTCGTTTGAAGTGTCAGGCGTTGCCCGT
TTCCGTGTGAATGCCTTTAATCACAATCGCGGTGCCGGTGCAGTGTTTCGTACGATTCCATCCAAGGTTTTGACGATGGA
TCAGCTCGGTATGGGGCAAGTCTTTAAAGACATGGCCAATATTTCGCGCGGCATTGTGCTGGTGACTGGGCCTACCGGTT
CAGGTAAGTCGACGACGTTAGCGGCTATGGTGGATTACATCAATGAAACCAAATACAACCATATTCTAACGGTTGAAGAC
CCGATTGAATTCGTACACGAATCCAAAAAAAGCCTGATCAACCAGCGTGAAGTGCATCGCGATACTCTAGGTTTTAATGA
AGCGTTACGCTCGGCGTTACGTGAAGACCCGGATGTGATTCTCGTGGGTGAGATGCGCGACTTAGAAACTATTCGTTTGG
CCTTAACCGCGGCAGAAACCGGTCACGTGGTATTTGGTACTTTGCATACCAGCTCGGCCGCGAAAACCATTGACCGTATT
GTCGACGTGTTTCCGGCAGAAGAAAAATCAATGGTGCGCTCAATGTTATCCGAGTCGCTACAAGGCGTTGTGTCTCAGGG
TTTGTTAAAGAAAAACGGCGGTGGTCGAATTGCGGCTCATGAAATCATGATTGGTACACCAGCGATTCGAAATTTGATTC
GTGAAGACAAAGTGGCGCAAATGTACTCGTCGATACAGACTGGCGCGAGTTACGGTATGCAGACTATGGATCAGTGCTTG
CAAAACATGGTCGGTAAAGGATTAATATCTCGTGAGACCGCACGAGAAAAAGCCAAAATGCCAGAAAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M5E9B4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

86.628

100

0.866
  pilT Pseudomonas stutzeri DSM 10701

85.465

100

0.855
  pilT Acinetobacter baumannii D1279779

81.977

100

0.82
  pilT Acinetobacter baumannii strain A118

81.977

100

0.82
  pilT Acinetobacter nosocomialis M2

81.977

100

0.82
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.882

98.837

0.651
  pilT Vibrio cholerae strain A1552

65.882

98.837

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384

Multiple sequence alignment