Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TOL_RS17025 Genome accession   NC_020888
Coordinates   3699237..3700352 (-) Length   371 a.a.
NCBI ID   WP_015488610.1    Uniprot ID   M5DWG0
Organism   Thalassolituus oleivorans MIL-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3694237..3705352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL_RS16985 (TOL_3517) - 3694649..3695143 (+) 495 WP_015488602.1 GNAT family N-acetyltransferase -
  TOL_RS16990 (TOL_3518) - 3695173..3695457 (-) 285 WP_025266500.1 DUF6916 family protein -
  TOL_RS16995 (TOL_3519) moaA 3695852..3696826 (+) 975 WP_015488604.1 GTP 3',8-cyclase MoaA -
  TOL_RS17000 (TOL_3520) mobA 3696828..3697421 (+) 594 WP_015488605.1 molybdenum cofactor guanylyltransferase -
  TOL_RS17005 (TOL_3521) moaB 3697423..3697965 (+) 543 WP_015488606.1 molybdenum cofactor biosynthesis protein B -
  TOL_RS17010 (TOL_3522) moaC 3697972..3698460 (+) 489 WP_015488607.1 cyclic pyranopterin monophosphate synthase MoaC -
  TOL_RS17015 (TOL_3523) - 3698457..3698705 (+) 249 WP_015488608.1 MoaD/ThiS family protein -
  TOL_RS17020 (TOL_3524) moaE 3698707..3699162 (+) 456 WP_015488609.1 molybdopterin synthase catalytic subunit MoaE -
  TOL_RS17025 (TOL_3525) pilU 3699237..3700352 (-) 1116 WP_015488610.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TOL_RS17030 (TOL_3526) pilT 3700365..3701399 (-) 1035 WP_015488611.1 type IV pilus twitching motility protein PilT Machinery gene
  TOL_RS17035 (TOL_3527) - 3701559..3702245 (+) 687 WP_015488612.1 YggS family pyridoxal phosphate-dependent enzyme -
  TOL_RS17040 (TOL_3528) proC 3702310..3703128 (+) 819 WP_015488613.1 pyrroline-5-carboxylate reductase -
  TOL_RS17045 (TOL_3529) - 3703225..3703812 (+) 588 WP_015488614.1 YggT family protein -
  TOL_RS17050 (TOL_3530) - 3703816..3704127 (+) 312 WP_015488615.1 DUF167 family protein -
  TOL_RS17055 (TOL_3531) metX 3704185..3705351 (+) 1167 WP_015488616.1 homoserine O-succinyltransferase MetX -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41407.43 Da        Isoelectric Point: 6.6923

>NTDB_id=57644 TOL_RS17025 WP_015488610.1 3699237..3700352(-) (pilU) [Thalassolituus oleivorans MIL-1]
MSMEKFLRLMVEKSASDLFITAGVPPSMKVHGKLVPLNKTVLSAEQTREIVVGMMNERQQHEFEEKKELNFAVNASGIGR
FRASAFFQRNTAGMVLRRIETRIPSVDQLGLPDIIKDLSMSKRGLILFVGATGAGKSTSLAAMIGHRNRNSKGHILSIED
PIEFIHQHDNCIVTQREVGLDTETFAVALKNCLRQAPDVIMIGEVRSAETMEHAITFAETGHLCLATLHGNNANQALDRV
IHFFPQERHSQIWMDLSLNLRAIVAQQLIPTPDGRGRRAVVEVLLNTPLVSDLIRKGDVHELKALMARSNELGMQTYDQA
LYSLYEQSEITYEDAIAHADSPNDLRLMIKLGNETNADRFDGSSNNLRLQD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=57644 TOL_RS17025 WP_015488610.1 3699237..3700352(-) (pilU) [Thalassolituus oleivorans MIL-1]
GTGTCAATGGAAAAATTTTTACGGCTTATGGTCGAAAAGAGTGCCTCAGACCTGTTTATTACCGCAGGTGTGCCTCCTTC
GATGAAAGTGCACGGTAAACTGGTGCCTTTAAATAAGACGGTATTGAGTGCTGAACAAACCCGCGAAATTGTTGTTGGTA
TGATGAACGAACGTCAACAACATGAGTTCGAAGAAAAGAAAGAATTGAATTTCGCCGTTAACGCCTCAGGTATTGGGCGT
TTTCGTGCGAGTGCGTTTTTTCAGCGCAACACAGCAGGCATGGTGTTACGTCGAATCGAAACCCGTATTCCTAGCGTTGA
TCAATTAGGCTTACCCGACATTATTAAAGACTTATCAATGTCGAAGCGTGGTTTGATTCTATTTGTGGGGGCAACGGGGG
CCGGTAAATCAACTTCGCTAGCCGCGATGATTGGTCATCGTAACCGTAATAGCAAAGGCCACATCTTAAGTATCGAAGAT
CCGATTGAATTTATTCATCAGCATGACAATTGTATCGTTACCCAGCGCGAAGTGGGTTTGGATACGGAAACCTTTGCGGT
TGCCTTAAAAAATTGTCTGCGTCAGGCGCCCGACGTCATTATGATTGGTGAGGTGCGTTCAGCAGAAACAATGGAACATG
CGATTACCTTCGCAGAAACCGGTCACTTGTGTTTGGCGACCTTACACGGTAATAACGCTAACCAAGCACTGGATCGTGTT
ATTCACTTCTTTCCACAAGAACGTCATTCGCAAATTTGGATGGATTTATCGCTTAACTTGCGTGCCATTGTTGCTCAGCA
ATTAATTCCTACCCCTGATGGTCGTGGCCGTCGTGCCGTGGTGGAAGTGTTGCTGAACACCCCTTTGGTGTCTGACTTGA
TTCGAAAAGGCGATGTACACGAATTGAAGGCTCTGATGGCGCGTTCAAACGAACTCGGTATGCAGACTTACGATCAAGCT
CTTTATTCCTTGTACGAGCAAAGCGAAATCACTTACGAAGATGCAATTGCTCACGCGGATTCACCAAACGATTTACGTTT
AATGATCAAGCTTGGCAATGAAACCAATGCGGATCGTTTTGATGGTTCTTCGAACAACTTACGTTTGCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M5DWG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

73.243

99.73

0.73
  pilU Acinetobacter baylyi ADP1

58.747

100

0.606
  pilU Vibrio cholerae strain A1552

55.285

99.461

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.978

96.226

0.423
  pilT Legionella pneumophila strain Lp02

41.194

90.296

0.372
  pilT Legionella pneumophila strain ERS1305867

41.194

90.296

0.372
  pilT Pseudomonas aeruginosa PAK

39.13

92.992

0.364
  pilT Acinetobacter baumannii strain A118

39.181

92.183

0.361
  pilT Acinetobacter nosocomialis M2

39.181

92.183

0.361
  pilT Acinetobacter baumannii D1279779

39.181

92.183

0.361

Multiple sequence alignment