Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KNN15_RS04970 Genome accession   NZ_CP076325
Coordinates   899816..900904 (-) Length   362 a.a.
NCBI ID   WP_028494590.1    Uniprot ID   -
Organism   MAG: Thermus antranikianii isolate RBS10-92     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 894816..905904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KNN15_RS04955 (KNN15_04895) - 896743..897372 (-) 630 WP_028494587.1 histidine phosphatase family protein -
  KNN15_RS04960 (KNN15_04900) purM 897369..898370 (-) 1002 WP_273107036.1 phosphoribosylformylglycinamidine cyclo-ligase -
  KNN15_RS04965 (KNN15_04905) gatB 898376..899785 (-) 1410 WP_273107039.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  KNN15_RS04970 (KNN15_04910) pilT 899816..900904 (-) 1089 WP_028494590.1 type IV pilus twitching motility protein PilT Machinery gene
  KNN15_RS04975 (KNN15_04915) pilF 900916..903585 (-) 2670 WP_273107041.1 type IV pilus assembly ATPase PilB Machinery gene
  KNN15_RS04980 (KNN15_04920) - 903582..904064 (-) 483 WP_273107044.1 YqeG family HAD IIIA-type phosphatase -
  KNN15_RS04985 (KNN15_04925) pgeF 904099..904824 (-) 726 WP_273107047.1 peptidoglycan editing factor PgeF -
  KNN15_RS04990 (KNN15_04930) - 904904..905308 (+) 405 WP_273107050.1 DUF4395 family protein -
  KNN15_RS04995 (KNN15_04935) ribH 905295..905768 (-) 474 WP_273107053.1 6,7-dimethyl-8-ribityllumazine synthase -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40114.45 Da        Isoelectric Point: 7.2367

>NTDB_id=574676 KNN15_RS04970 WP_028494590.1 899816..900904(-) (pilT) [MAG: Thermus antranikianii isolate RBS10-92]
MAKTPDIVDLLTLAVERGASDLVITVGLPPMIKVDGEFHPTEYEPLSPQDTRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPATIKSFEELGLPKTIAEIAMAPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHKHKKAIVNQREIGSDTHSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMIT
MDACLADLYKRKLITYEMGLSRAVDPKEFMRLAGVQEGSKRP

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=574676 KNN15_RS04970 WP_028494590.1 899816..900904(-) (pilT) [MAG: Thermus antranikianii isolate RBS10-92]
ATGGCGAAAACACCGGACATCGTCGATCTTCTGACCCTGGCTGTAGAGCGAGGGGCCAGCGATTTGGTGATCACCGTGGG
CCTTCCCCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTTTCTCCCCAGGATACCCGCCGGC
TCATGTACGCCCTCATGGACGAAAAGCAGCAGCGGATCTTTGAAGAGGAAAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGAAAAGGGCGGTACCGGGTAAACGTTTTCCTCCAACGGGGAAGCGTGGGGGGGGTCTTAAGGGTGGTGCCCGCCACCAT
CAAGAGCTTTGAGGAACTCGGCTTGCCCAAGACCATCGCCGAGATCGCCATGGCTCCCAGGGGCCTGGTCCTGGTCACGG
GGCCCACCGGGTCGGGGAAGAGCACCACCTTAGCCTCCATGATCGACTACATCAACGAACGCAAGCCCGTGCACATCGTG
ACCATTGAGGACCCCATAGAGTTCTTCCACAAGCACAAAAAGGCCATCGTCAACCAACGGGAGATCGGCTCCGATACCCA
CAGCTTCCACAAGGCGCTTAGAAGCGTTCTCCGCCAGGCCCCGGATGTGATCCTGGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCGGAAACCGGGCATTTGGTCATGGGCACCCTGCACACCAACTCTGCCCCCGAGACC
ATCGACCGCATCATCGACGTCTTCCCGGAAAACCAGCAGGAGCAGGTGCGGGTGCAGCTTTCCAACAACCTGGTGGCGGT
GCTCACCCAGCAGCTTCTGCCCAAGGCCTTCGGGGGCGGAAGGGTCTTGGCCTACGAGCTCATGATCGCCACCCCTGCGG
TAAGGGCCTTGATCCGGGAGGGCAAAAGCCACCAGCTCCGGAGCGTCATCCAGACCGGGGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTGGCGGATCTCTACAAGCGCAAGTTGATCACCTACGAGATGGGCTTGAGCCGGGCGGTGGACCCCAA
GGAGTTCATGCGCCTGGCTGGGGTGCAGGAGGGGAGCAAGCGCCCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

68.839

97.514

0.671
  pilT Pseudomonas aeruginosa PAK

54.07

95.028

0.514
  pilT Acinetobacter nosocomialis M2

53.913

95.304

0.514
  pilT Acinetobacter baumannii strain A118

53.913

95.304

0.514
  pilT Acinetobacter baumannii D1279779

53.913

95.304

0.514
  pilT Pseudomonas stutzeri DSM 10701

53.779

95.028

0.511
  pilT Acinetobacter baylyi ADP1

53.779

95.028

0.511
  pilT Legionella pneumophila strain Lp02

52.035

95.028

0.494
  pilT Legionella pneumophila strain ERS1305867

52.035

95.028

0.494
  pilT Neisseria meningitidis 8013

52.663

93.37

0.492
  pilT Neisseria gonorrhoeae MS11

51.929

93.094

0.483
  pilT Vibrio cholerae strain A1552

52.888

90.884

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.888

90.884

0.481
  pilU Pseudomonas stutzeri DSM 10701

47.813

94.751

0.453
  pilU Vibrio cholerae strain A1552

45.21

92.265

0.417
  pilU Acinetobacter baylyi ADP1

41.341

98.895

0.409