Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KNN15_RS04975 Genome accession   NZ_CP076325
Coordinates   900916..903585 (-) Length   889 a.a.
NCBI ID   WP_273107041.1    Uniprot ID   -
Organism   MAG: Thermus antranikianii isolate RBS10-92     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 895916..908585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KNN15_RS04955 (KNN15_04895) - 896743..897372 (-) 630 WP_028494587.1 histidine phosphatase family protein -
  KNN15_RS04960 (KNN15_04900) purM 897369..898370 (-) 1002 WP_273107036.1 phosphoribosylformylglycinamidine cyclo-ligase -
  KNN15_RS04965 (KNN15_04905) gatB 898376..899785 (-) 1410 WP_273107039.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  KNN15_RS04970 (KNN15_04910) pilT 899816..900904 (-) 1089 WP_028494590.1 type IV pilus twitching motility protein PilT Machinery gene
  KNN15_RS04975 (KNN15_04915) pilF 900916..903585 (-) 2670 WP_273107041.1 type IV pilus assembly ATPase PilB Machinery gene
  KNN15_RS04980 (KNN15_04920) - 903582..904064 (-) 483 WP_273107044.1 YqeG family HAD IIIA-type phosphatase -
  KNN15_RS04985 (KNN15_04925) pgeF 904099..904824 (-) 726 WP_273107047.1 peptidoglycan editing factor PgeF -
  KNN15_RS04990 (KNN15_04930) - 904904..905308 (+) 405 WP_273107050.1 DUF4395 family protein -
  KNN15_RS04995 (KNN15_04935) ribH 905295..905768 (-) 474 WP_273107053.1 6,7-dimethyl-8-ribityllumazine synthase -
  KNN15_RS05000 (KNN15_04940) crcB 905789..906166 (+) 378 WP_028494596.1 fluoride efflux transporter CrcB -
  KNN15_RS05005 (KNN15_04945) - 906171..906503 (+) 333 WP_273107057.1 DUF190 domain-containing protein -
  KNN15_RS05010 (KNN15_04950) - 906548..907162 (+) 615 WP_028494598.1 sulfite oxidase-like oxidoreductase -
  KNN15_RS05015 (KNN15_04955) - 907159..907428 (+) 270 WP_028494599.1 hypothetical protein -
  KNN15_RS05020 (KNN15_04960) - 907404..908207 (-) 804 WP_028494600.1 histidinol-phosphatase HisJ family protein -

Sequence

Protein


Download         Length: 889 a.a.        Molecular weight: 98119.13 Da        Isoelectric Point: 5.0554

>NTDB_id=574677 KNN15_RS04975 WP_273107041.1 900916..903585(-) (pilF) [MAG: Thermus antranikianii isolate RBS10-92]
MSVLTIGDKRLGAILLDAGLLTDEELQMALEKHREVGGSLAEVIVDSGLLSERRIAQAIEDHFGIPLVELHTLEIPPKVK
ALLPAEKAKELQAIPFALDEEAGVVRVAFVNPLDTLALEEVEDLTGMVVEPYQATKSAFLYALAKHYPELNLPLPPPPSG
PSREELRVGELLVEKGLLDRNTLEEALVEQEKTGDLLGRILVRKGLPEEVLYRVLAEQKGMEFLPSTQGLSPDPAATNLL
LRSDALRYSAVPVGFNNGEVEVVLADPRHKEAVAELLGRPARFFLTLPKEWEALFHQAYPEKSRLGEVLVQEGRLSREDL
REALEVQRRLPKAKPLGEILVELGLARPEDVEEALKKQRQGGGRLEDTLVASGKLRPEALAQAVATQLGYAYINPEENPP
DPGAALLLPEDLARRYGIFPHRLEGKTLVLLMKDPRNILALDDVRLALKRKGLAYEVQPAVATEAAITKLIERFYGKEEL
GEIAKELSKGYQEEEEAVTPELDESAAQKFVKQVIREAFLQDASDIHIEPRQSDVLVRLRIDGVLRQYTTLPKGALNSII
SVVKIMGGLNIAERRLPQDGRVRYREGSIDVDLRLSTLPTVYGEKAVMRLLKKAADIPEIEGLGFAPGVFERFQEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRICDHCKVEVKPDPEVLRRLGL
SEEEIQGAKLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARKKGMKTLREDGIYKALQGITTL
EEVLARTIE

Nucleotide


Download         Length: 2670 bp        

>NTDB_id=574677 KNN15_RS04975 WP_273107041.1 900916..903585(-) (pilF) [MAG: Thermus antranikianii isolate RBS10-92]
ATGAGCGTGCTCACCATCGGGGACAAACGGCTAGGTGCCATCCTATTGGATGCCGGCCTCCTCACCGACGAGGAGCTGCA
GATGGCCCTGGAGAAGCACCGGGAGGTGGGGGGTTCCTTGGCCGAGGTCATCGTGGACTCGGGGCTGCTTTCGGAAAGGC
GGATCGCCCAGGCCATCGAGGACCACTTCGGCATCCCCCTGGTGGAGCTCCACACTTTGGAGATCCCCCCCAAGGTGAAG
GCCCTCCTGCCGGCGGAGAAGGCCAAGGAACTCCAGGCCATCCCCTTCGCCCTGGACGAGGAGGCAGGGGTGGTGCGGGT
GGCCTTCGTAAACCCCCTGGACACCTTGGCCCTCGAGGAGGTGGAGGACCTCACCGGGATGGTGGTGGAACCCTACCAAG
CCACCAAGAGCGCCTTCCTCTACGCCTTGGCCAAGCACTACCCCGAGCTCAACCTGCCCCTGCCTCCTCCCCCCAGCGGC
CCCAGCCGGGAGGAGCTGAGGGTGGGCGAGCTTCTGGTGGAAAAGGGCCTCCTGGACCGGAACACCCTGGAGGAAGCCCT
GGTGGAGCAGGAAAAGACCGGGGATCTCCTGGGCCGCATCCTGGTGCGCAAGGGGCTACCCGAGGAAGTCCTCTACCGGG
TTTTGGCCGAGCAGAAGGGAATGGAATTTCTCCCCTCCACCCAGGGCCTATCCCCCGATCCGGCCGCCACCAACCTTCTC
CTGCGCTCAGATGCCCTGCGCTATAGCGCCGTGCCCGTGGGCTTCAACAACGGGGAGGTGGAGGTGGTCCTCGCCGATCC
CCGTCACAAGGAGGCAGTGGCGGAGCTCCTTGGGCGCCCCGCCCGCTTCTTCCTCACCCTTCCCAAGGAGTGGGAAGCCC
TTTTCCACCAGGCCTATCCGGAAAAAAGCCGCCTGGGGGAGGTCCTGGTCCAGGAGGGGCGCCTGAGCCGGGAGGATTTA
AGAGAGGCCCTCGAGGTGCAAAGGCGTCTGCCCAAGGCCAAGCCCCTGGGGGAGATCCTGGTGGAGCTGGGCTTGGCCCG
GCCCGAGGACGTGGAGGAAGCCCTGAAGAAGCAACGCCAGGGTGGAGGCCGCCTCGAGGACACCCTGGTGGCCTCGGGCA
AGCTCAGGCCCGAAGCCCTGGCTCAGGCGGTGGCCACCCAGCTGGGCTACGCCTACATCAACCCCGAGGAGAACCCTCCG
GATCCCGGGGCCGCCCTCCTCCTCCCCGAGGACCTGGCCCGGCGGTACGGGATCTTCCCCCACCGCCTGGAGGGCAAGAC
CCTGGTCCTCCTCATGAAGGATCCCAGGAACATCCTGGCCCTGGACGACGTGCGCTTGGCCCTTAAGCGCAAGGGCCTGG
CCTACGAGGTTCAGCCCGCCGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAACGCTTCTACGGCAAGGAGGAACTC
GGCGAGATCGCCAAGGAGCTGTCCAAGGGCTACCAGGAGGAGGAGGAAGCCGTCACCCCTGAGCTGGACGAAAGCGCCGC
CCAGAAGTTTGTCAAACAGGTAATCCGGGAGGCCTTCCTGCAGGATGCCTCCGACATCCACATAGAACCCCGCCAGTCCG
ACGTCCTGGTGCGCCTTCGCATCGACGGGGTCTTGCGCCAGTACACCACCCTGCCCAAGGGGGCCCTAAACTCCATCATC
AGCGTGGTCAAGATCATGGGCGGCCTCAACATCGCCGAAAGGCGCCTGCCCCAGGACGGGCGGGTGCGCTACCGGGAAGG
TTCCATCGACGTGGACCTCCGCCTCTCCACCCTGCCCACGGTCTACGGGGAAAAGGCGGTGATGCGCCTTCTGAAAAAGG
CCGCCGACATCCCCGAGATCGAGGGCCTGGGCTTCGCCCCTGGGGTCTTTGAGCGCTTCCAGGAGGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGGCCCACCGGAAGCGGCAAAAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATCGAGGACCCCGTGGAGTACGAGATCCCTGGCATCAACCAGACCCAGGTGAACCCCC
AAGCTGGCCTCACCTTTGCCCGGGCCCTCAGGGCCTTCCTAAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGG
GACTCGGAAACCGCCAAGATCGCCACCGAGGCCGCTCTCACCGGCCACCTGGTCATCGCCACCCTGCACACCAACGATGC
TGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAACCCTTCAACATCTCCGCCGCCCTCATCGGGGTTCTCTCCC
AGCGTCTGGTGCGGAGGATCTGCGACCACTGCAAGGTAGAGGTGAAGCCCGACCCCGAGGTCCTGCGGCGCTTAGGCCTT
TCCGAAGAGGAGATCCAAGGGGCCAAGCTCTACAAGGGGATGGGATGCGAGCGCTGCGGGGGAACGGGCTATAAGGGGCG
CTACGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCCGGCAAGTCGGCCACGGAGATCA
AGGAGATCGCCAGGAAAAAGGGCATGAAAACCCTGCGGGAGGACGGCATCTACAAGGCCCTCCAGGGAATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(173-303)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-148)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(369-479)

Type II/IV secretion system protein (T2SSE)

(513-779)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

88.076

100

0.881
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.629

100

0.567