Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H681_RS01795 Genome accession   NC_020829
Coordinates   392378..393412 (+) Length   344 a.a.
NCBI ID   WP_015475130.1    Uniprot ID   M4WU88
Organism   Pseudomonas sp. ATCC 13867     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 387378..398412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H681_RS01770 (H681_01775) - 387774..389741 (-) 1968 WP_015475125.1 dynamin-like GTPase family protein -
  H681_RS01775 (H681_01780) - 389859..390149 (-) 291 WP_015475126.1 DUF167 domain-containing protein -
  H681_RS01780 (H681_01785) - 390157..390750 (-) 594 WP_015475127.1 YggT family protein -
  H681_RS01785 (H681_01790) proC 390764..391597 (-) 834 WP_015475128.1 pyrroline-5-carboxylate reductase -
  H681_RS01790 (H681_01795) - 391615..392304 (-) 690 WP_015475129.1 YggS family pyridoxal phosphate-dependent enzyme -
  H681_RS01795 (H681_01800) pilT 392378..393412 (+) 1035 WP_015475130.1 type IV pilus twitching motility protein PilT Machinery gene
  H681_RS01800 (H681_01805) pilU 393517..394662 (+) 1146 WP_015475131.1 type IV pilus ATPase PilU Machinery gene
  H681_RS01805 (H681_01810) - 394738..395142 (+) 405 WP_015475132.1 TM2 domain-containing protein -
  H681_RS01810 (H681_01815) - 395336..396709 (+) 1374 WP_015475133.1 cystathionine beta-synthase -
  H681_RS01815 (H681_01820) - 396706..397875 (+) 1170 WP_015475134.1 PLP-dependent aspartate aminotransferase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37868.73 Da        Isoelectric Point: 6.7752

>NTDB_id=57441 H681_RS01795 WP_015475130.1 392378..393412(+) (pilT) [Pseudomonas sp. ATCC 13867]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDVPRGLVLVTGPTGSGKSTSLAAMLDYLNNTKYSHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGAIGMQTLDMCL
KGLVAKGLVTRDAAKEKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=57441 H681_RS01795 WP_015475130.1 392378..393412(+) (pilT) [Pseudomonas sp. ATCC 13867]
ATGGATATTACCGAGTTGCTCGCCTTCAGCGCCAAGCAGGGCGCTTCGGACCTGCACCTCTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGACGGTGATGTTCGCCGGATCAACCTGCCGCCCCTGGAGCACAAGCAGGTCCATGCGCTGATCTATGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAAGAATTTCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGGGTCGCCCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTACTCACGATGGA
GGACCTGGGGATGGGAGAAGTGTTCAAACGTGTTTCAGACGTGCCACGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACTTCGCTGGCGGCGATGCTCGACTACCTGAACAACACCAAGTACAGCCACATTCTCACTGTCGAAGAC
CCGATCGAATTCGTCCATGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAAGTGCACCGCGACACCCTCGGCTTCAACGA
GGCGCTGCGCTCGGCCCTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTCTTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGCCGAGGAGAAGTCGATGGTCCGCTCCATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGGGTGGCGGCCCACGAGATCATGATCGGTACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCCATCCAGACCGGCGGCGCGATCGGCATGCAGACCCTGGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGGCTGGTCACTCGCGATGCGGCGAAAGAAAAAGCCAAGATTCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M4WU88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.349

100

0.953
  pilT Pseudomonas stutzeri DSM 10701

93.314

100

0.933
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

76.036

98.256

0.747
  pilT Legionella pneumophila strain ERS1305867

76.036

98.256

0.747
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

98.837

0.677
  pilT Vibrio cholerae strain A1552

68.529

98.837

0.677
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment