Detailed information NTG0057442

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H681_RS01800
• Genome accession: NC_020829
• Coordinates: 393517..394662 (+)
• Length: 381 a.a.
• NCBI ID: WP_015475131.1
• Uniprot ID: -
• Organism: Pseudomonas sp. ATCC 13867
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 388517..399662

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H681_RS01775 (H681_01780)	-	389859..390149 (-)	291	WP_015475126.1	DUF167 domain-containing protein	-
H681_RS01780 (H681_01785)	-	390157..390750 (-)	594	WP_015475127.1	YggT family protein	-
H681_RS01785 (H681_01790)	proC	390764..391597 (-)	834	WP_015475128.1	pyrroline-5-carboxylate reductase	-
H681_RS01790 (H681_01795)	-	391615..392304 (-)	690	WP_015475129.1	YggS family pyridoxal phosphate-dependent enzyme	-
H681_RS01795 (H681_01800)	pilT	392378..393412 (+)	1035	WP_015475130.1	type IV pilus twitching motility protein PilT	Machinery gene
H681_RS01800 (H681_01805)	pilU	393517..394662 (+)	1146	WP_015475131.1	type IV pilus ATPase PilU	Machinery gene
H681_RS01805 (H681_01810)	-	394738..395142 (+)	405	WP_015475132.1	TM2 domain-containing protein	-
H681_RS01810 (H681_01815)	-	395336..396709 (+)	1374	WP_015475133.1	cystathionine beta-synthase	-
H681_RS01815 (H681_01820)	-	396706..397875 (+)	1170	WP_015475134.1	PLP-dependent aspartate aminotransferase family protein	-
H681_RS01820 (H681_01825)	-	398088..399359 (-)	1272	WP_015475135.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42556.86 Da
• Isoelectric Point: 6.7258

>NTDB_id=57442 H681_RS01800 WP_015475131.1 393517..394662(+) (pilU) [Pseudomonas sp. ATCC 13867]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKVLPVTKTALSPEQTRETVLSVMNEQQRREFAENHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETNIPTIEDLKLPEVLKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDTFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSSMTQGLTLEMSDDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=57442 H681_RS01800 WP_015475131.1 393517..394662(+) (pilU) [Pseudomonas sp. ATCC 13867]
ATGGAATTCGAGAAGCTGTTGCGCCTGATGGTGGAAAAGGGGGCCTCCGACCTGTTCATCACGGCGGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGGTGTTGCCGGTGACCAAGACCGCGCTGTCGCCGGAGCAGACCCGCGAAACCGTGCTCTCGG
TGATGAACGAACAGCAACGCCGCGAGTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGTGGCGTCGGCCGT
TTCCGGGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAATATCCCGACCATCGA
AGACCTCAAGCTGCCGGAAGTCCTCAAGAAACTGGCGATGACCAAGCGTGGCCTGGTGATCTTCGTCGGCGCCACCGGTA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGCCACATCATCTCCATCGAAGAC
CCTATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGCCTGGATACCGATACCTTCGAAGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCATGGCCAGGTGTGGATGGACCTCTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTATTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAAGGCATGCAGACCTTCGACCAGGCG
CTGTACCAACTCTACAGCCAGGGCGAGATCACCTACGAAGACGCCCTGGCCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGATGCCGACCACCTGTCCAGCATGACCCAGGGGCTGACCCTGGAGATGAGCGACG
ACGACCCGAACCGCCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	89.239	100	0.892
pilU	Acinetobacter baylyi ADP1	63.298	98.688	0.625
pilU	Vibrio cholerae strain A1552	58.76	97.375	0.572
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.976	0.383