Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KM539_RS14330 Genome accession   NZ_CP076250
Coordinates   3290029..3291066 (-) Length   345 a.a.
NCBI ID   WP_053840294.1    Uniprot ID   A0A0K2ZNS6
Organism   Xanthomonas translucens pv. poae strain LMG 728     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3285029..3296066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM539_RS14310 (KM539_14260) - 3285463..3286029 (-) 567 WP_053840291.1 YqgE/AlgH family protein -
  KM539_RS14315 (KM539_14265) - 3286137..3287945 (+) 1809 WP_053840322.1 DUF4153 domain-containing protein -
  KM539_RS14320 (KM539_14270) - 3287961..3288500 (+) 540 WP_053840292.1 DNA-3-methyladenine glycosylase I -
  KM539_RS14325 (KM539_14275) pilU 3288757..3289887 (-) 1131 WP_053840293.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KM539_RS14330 (KM539_14280) pilT 3290029..3291066 (-) 1038 WP_053840294.1 type IV pilus twitching motility protein PilT Machinery gene
  KM539_RS14335 (KM539_14285) - 3291191..3291841 (+) 651 WP_053840323.1 YggS family pyridoxal phosphate-dependent enzyme -
  KM539_RS14340 (KM539_14290) - 3292324..3293379 (+) 1056 WP_170874270.1 hypothetical protein -
  KM539_RS14345 (KM539_14295) proC 3293509..3294351 (+) 843 WP_053840296.1 pyrroline-5-carboxylate reductase -
  KM539_RS14350 (KM539_14300) - 3294366..3294821 (+) 456 WP_053840297.1 DUF4426 domain-containing protein -
  KM539_RS14355 (KM539_14305) soxR 3294959..3295396 (-) 438 WP_053840298.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38627.30 Da        Isoelectric Point: 6.6650

>NTDB_id=574028 KM539_RS14330 WP_053840294.1 3290029..3291066(-) (pilT) [Xanthomonas translucens pv. poae strain LMG 728]
MDIAELLAFSVKNKASDLHLSVGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQHL
QDLVKRSLITRNQARDYAKDKRVFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=574028 KM539_RS14330 WP_053840294.1 3290029..3291066(-) (pilT) [Xanthomonas translucens pv. poae strain LMG 728]
ATGGATATCGCTGAATTGTTGGCGTTCTCGGTCAAGAACAAAGCGTCCGACCTGCACCTGTCCGTCGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGCATCAATATCCCGGCACTGGACCACAAGCAGGTGCATGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAAGAATTCCTCGAAGTGGACTTCTCGTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACCCTGGA
AGACCTCGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCTTGGTCACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGATTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
AGCGTTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCTTTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCGTGGGAGATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGTACGGCATGCAGACCCTGGACCAGCACCTG
CAGGATCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCGCGCGACTACGCCAAGGACAAGCGGGTCTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K2ZNS6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Vibrio cholerae strain A1552

40.517

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

39.943

100

0.403
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394