Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KM539_RS14325 Genome accession   NZ_CP076250
Coordinates   3288757..3289887 (-) Length   376 a.a.
NCBI ID   WP_053840293.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. poae strain LMG 728     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3283757..3294887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM539_RS14300 (KM539_14250) - 3284006..3284953 (-) 948 WP_053834932.1 aspartate carbamoyltransferase catalytic subunit -
  KM539_RS14305 (KM539_14255) ruvX 3284973..3285470 (-) 498 WP_053840290.1 Holliday junction resolvase RuvX -
  KM539_RS14310 (KM539_14260) - 3285463..3286029 (-) 567 WP_053840291.1 YqgE/AlgH family protein -
  KM539_RS14315 (KM539_14265) - 3286137..3287945 (+) 1809 WP_053840322.1 DUF4153 domain-containing protein -
  KM539_RS14320 (KM539_14270) - 3287961..3288500 (+) 540 WP_053840292.1 DNA-3-methyladenine glycosylase I -
  KM539_RS14325 (KM539_14275) pilU 3288757..3289887 (-) 1131 WP_053840293.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KM539_RS14330 (KM539_14280) pilT 3290029..3291066 (-) 1038 WP_053840294.1 type IV pilus twitching motility protein PilT Machinery gene
  KM539_RS14335 (KM539_14285) - 3291191..3291841 (+) 651 WP_053840323.1 YggS family pyridoxal phosphate-dependent enzyme -
  KM539_RS14340 (KM539_14290) - 3292324..3293379 (+) 1056 WP_170874270.1 hypothetical protein -
  KM539_RS14345 (KM539_14295) proC 3293509..3294351 (+) 843 WP_053840296.1 pyrroline-5-carboxylate reductase -
  KM539_RS14350 (KM539_14300) - 3294366..3294821 (+) 456 WP_053840297.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41956.32 Da        Isoelectric Point: 6.8810

>NTDB_id=574027 KM539_RS14325 WP_053840293.1 3288757..3289887(-) (pilU) [Xanthomonas translucens pv. poae strain LMG 728]
MSIIDFTSFLKLMAHQKASDLFITAGMPPAIKVHGKITPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNMRGVVAQQLIPTPDGKSRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFDLYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=574027 KM539_RS14325 WP_053840293.1 3288757..3289887(-) (pilU) [Xanthomonas translucens pv. poae strain LMG 728]
ATGAGCATCATCGACTTCACCTCATTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCGCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCGCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGTATCCC
CACCGTCGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATCTCCTTCGCCGAGACCGGCCACCTGGTGTTGTGCACCCTGCATGCCAACAACGCCAATCAGGCGATG
GACCGGATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACTTGTCGCTGAACATGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGAGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGATCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAGGACCCTGGCGCAGGGCATGGACGGGGTGGAAATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.583

98.138

0.644
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367