Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KME82_RS05980 Genome accession   NZ_CP076103
Coordinates   1478046..1479083 (+) Length   345 a.a.
NCBI ID   WP_036109068.1    Uniprot ID   A0A108U802
Organism   Lysobacter capsici strain NF87_2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1473046..1484083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KME82_RS05955 (KME82_05955) - 1473290..1474234 (+) 945 WP_215497712.1 triacylglycerol lipase -
  KME82_RS05960 (KME82_05960) - 1474238..1475257 (+) 1020 WP_215497713.1 lipase secretion chaperone -
  KME82_RS05965 (KME82_05965) - 1475507..1475980 (-) 474 WP_215497714.1 DUF4426 domain-containing protein -
  KME82_RS05970 (KME82_05970) proC 1475977..1476813 (-) 837 WP_215497715.1 pyrroline-5-carboxylate reductase -
  KME82_RS05975 (KME82_05975) - 1477169..1477870 (-) 702 WP_345777968.1 YggS family pyridoxal phosphate-dependent enzyme -
  KME82_RS05980 (KME82_05980) pilT 1478046..1479083 (+) 1038 WP_036109068.1 type IV pilus twitching motility protein PilT Machinery gene
  KME82_RS05985 (KME82_05985) pilU 1479191..1480357 (+) 1167 WP_046655750.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38546.39 Da        Isoelectric Point: 6.7270

>NTDB_id=571832 KME82_RS05980 WP_036109068.1 1478046..1479083(+) (pilT) [Lysobacter capsici strain NF87_2]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIEQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREIHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQGYGMMTLDQHL
QDLVKRGMILRPQAREYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=571832 KME82_RS05980 WP_036109068.1 1478046..1479083(+) (pilT) [Lysobacter capsici strain NF87_2]
ATGGATATCGCCGAACTACTGGCGTTCTCGGTTAAGAACAAAGCATCCGACTTGCATCTGTCGGCGGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGGATCAACATCCCGGCCCTGGACCACAAGCAGGTCCATGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTTCGAAGAATTCCTCGAGGTCGACTTCTCCTTCGAAATCCCTGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGTACCATTCCCTCCGAAGTGCTGACCCTGGA
AGACCTCGGCTGCCCGCCGATCTTCCGTCAGCTGATCGAACAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCGATGATCGACCACATCAACAAGAACGAGTACGCGCACATCCTGTCGGTGGAAGAC
CCGATCGAGTTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAATCCACCGCGACACGCACGGCTTCAACGA
AGCCTTGCGCTCGGCGTTGCGCGAAGATCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCCTCGGCGGCCAAGACCGTCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCGGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTCAAGAAGGTCGGCGGCGGCCGTACCGCGGCCTGGGAAATCATGGTCGGCATCCCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCCAGGGCTACGGCATGATGACGCTCGATCAGCATCTG
CAGGATCTGGTCAAGCGCGGCATGATCCTGCGCCCGCAGGCACGCGAGTACGCGAAGGACAAGCGCCTGTTCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A108U802

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

73.256

99.71

0.73
  pilT Legionella pneumophila strain ERS1305867

73.256

99.71

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.605

95.362

0.664
  pilT Vibrio cholerae strain A1552

69.605

95.362

0.664
  pilT Neisseria meningitidis 8013

65.789

99.13

0.652
  pilT Neisseria gonorrhoeae MS11

65.497

99.13

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38