Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KME82_RS05985 Genome accession   NZ_CP076103
Coordinates   1479191..1480357 (+) Length   388 a.a.
NCBI ID   WP_046655750.1    Uniprot ID   -
Organism   Lysobacter capsici strain NF87_2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1474191..1485357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KME82_RS05960 (KME82_05960) - 1474238..1475257 (+) 1020 WP_215497713.1 lipase secretion chaperone -
  KME82_RS05965 (KME82_05965) - 1475507..1475980 (-) 474 WP_215497714.1 DUF4426 domain-containing protein -
  KME82_RS05970 (KME82_05970) proC 1475977..1476813 (-) 837 WP_215497715.1 pyrroline-5-carboxylate reductase -
  KME82_RS05975 (KME82_05975) - 1477169..1477870 (-) 702 WP_345777968.1 YggS family pyridoxal phosphate-dependent enzyme -
  KME82_RS05980 (KME82_05980) pilT 1478046..1479083 (+) 1038 WP_036109068.1 type IV pilus twitching motility protein PilT Machinery gene
  KME82_RS05985 (KME82_05985) pilU 1479191..1480357 (+) 1167 WP_046655750.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43133.57 Da        Isoelectric Point: 6.8286

>NTDB_id=571833 KME82_RS05985 WP_046655750.1 1479191..1480357(+) (pilU) [Lysobacter capsici strain NF87_2]
MNTPAPTNPTTASGAIDFTSFLKLMAHQRASDLFITSGMPPSMKVHGKISPITQNPLTPQQSRDLVLNVMNPQQREEFEK
THECNFAIGVTGIGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIG
YRNLNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVL
CTLHANNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKEL
MKDSVQLGMKTFDQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=571833 KME82_RS05985 WP_046655750.1 1479191..1480357(+) (pilU) [Lysobacter capsici strain NF87_2]
ATGAATACCCCGGCCCCGACCAACCCGACCACCGCCTCCGGCGCGATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCA
CCAGCGCGCCTCGGACTTGTTCATCACCTCGGGCATGCCCCCGTCGATGAAGGTCCACGGCAAGATCAGCCCGATCACCC
AGAACCCGCTGACGCCGCAGCAAAGCCGCGACCTCGTGCTCAACGTGATGAATCCGCAGCAGCGCGAAGAGTTCGAAAAG
ACCCACGAGTGCAACTTCGCCATCGGCGTGACCGGCATCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGT
CGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCCGACCGTCGAAGAACTGAACCTGCCGCCGATCATCAAGACGCTGG
CGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCGACCGGCACCGGCAAGTCGACCTCGCTCGCGGCGATGATCGGC
TACCGCAATCTGAATTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCATCGCCACGAAGGCTGCAT
CATCACCCAGCGCGAAGTCGGCATCGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACG
TGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGTCACCTGGTGCTG
TGCACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGTCGCAACCAGTT
GCTGATGGACTTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGATCCCGACCCCCGACGGCAAGGGCCGCCGCG
TCGCCATGGAAATCCTGCTCGGCACCCCGCTGGCCCAGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAAGAACTG
ATGAAGGACTCCGTGCAGCTCGGCATGAAGACCTTCGACCAGGCGCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTA
CGAAGACGCCCTGCGCTACGCCGACTCGCAGAACGAAGTGCGTTTGCGCATCAAGCTGGCGCAAGGTGGCGATGCGCGGA
CCTTGTCGCAGGGGTTGGATGGGGTTGAGGTGGCTGAGGTCAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.755

94.588

0.66
  pilU Acinetobacter baylyi ADP1

63.866

92.01

0.588
  pilU Vibrio cholerae strain A1552

53.652

91.753

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.165

90.464

0.381