Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KKZ03_RS05565 Genome accession   NZ_CP076024
Coordinates   1204324..1205361 (-) Length   345 a.a.
NCBI ID   WP_243220546.1    Uniprot ID   -
Organism   Methylobacter sp. S3L5C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1199324..1210361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKZ03_RS05540 (KKZ03_05540) - 1200301..1200891 (+) 591 WP_243220542.1 aminodeoxychorismate/anthranilate synthase component II -
  KKZ03_RS05545 (KKZ03_05545) - 1200913..1201143 (+) 231 WP_243220543.1 DUF2442 domain-containing protein -
  KKZ03_RS05550 (KKZ03_05550) trpD 1201180..1202196 (+) 1017 WP_243220544.1 anthranilate phosphoribosyltransferase -
  KKZ03_RS05555 (KKZ03_05555) trpC 1202210..1203010 (+) 801 WP_243220545.1 indole-3-glycerol phosphate synthase TrpC -
  KKZ03_RS05560 (KKZ03_05560) pilU 1203156..1204313 (-) 1158 WP_243221543.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KKZ03_RS05565 (KKZ03_05565) pilT 1204324..1205361 (-) 1038 WP_243220546.1 type IV pilus twitching motility protein PilT Machinery gene
  KKZ03_RS05570 (KKZ03_05570) - 1205488..1206159 (+) 672 WP_243220547.1 YggS family pyridoxal phosphate-dependent enzyme -
  KKZ03_RS05575 (KKZ03_05575) - 1206172..1206876 (-) 705 WP_243220548.1 hypothetical protein -
  KKZ03_RS21975 - 1207477..1208813 (-) 1337 Protein_1105 IS4 family transposase -
  KKZ03_RS05590 (KKZ03_05590) - 1208959..1209087 (-) 129 Protein_1106 group II intron maturase-specific domain-containing protein -
  KKZ03_RS05595 - 1209442..1209621 (-) 180 WP_243218224.1 hypothetical protein -
  KKZ03_RS05600 (KKZ03_05595) - 1209631..1210188 (-) 558 WP_243220549.1 transposase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38406.23 Da        Isoelectric Point: 7.0746

>NTDB_id=571347 KKZ03_RS05565 WP_243220546.1 1204324..1205361(-) (pilT) [Methylobacter sp. S3L5C]
MDIAELLVFSVKNKASDLHLSAGLPPMIRVDGDIKKINLPALDHKAVHALIYDIMNDKQRRDYEEFLETDFSFELPGIAR
FRVNAFNQQRGAAAVFRTIPSKVLSLEDLNAPKFFEELTRKPRGLILVTGPTGSGKSTTLAAMINHINCNDYAHILTVED
PIEFVHDSQKCLINQREVHRDTHGFNEALRSALREDPDIIMVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLMKKTGGGRVAAHEIMVGTSAIRNLIREAKVAQMYSAIQTGRKEGMQTLDQNL
RELVDKGLVTSKEAMVKAVNKDSFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=571347 KKZ03_RS05565 WP_243220546.1 1204324..1205361(-) (pilT) [Methylobacter sp. S3L5C]
ATGGATATTGCAGAGTTACTGGTCTTTTCGGTTAAAAATAAAGCGTCAGATTTACATTTATCAGCAGGTCTGCCACCCAT
GATCCGGGTTGATGGTGATATAAAGAAAATTAATCTGCCGGCTCTCGACCATAAGGCAGTGCATGCTTTAATTTATGACA
TCATGAATGACAAGCAACGTCGTGATTATGAAGAGTTTTTGGAGACTGACTTTTCGTTTGAATTGCCTGGAATAGCGAGG
TTTCGTGTTAATGCCTTTAATCAGCAACGTGGGGCAGCGGCAGTTTTTAGAACCATTCCCTCAAAAGTATTGTCTTTGGA
AGACCTGAATGCTCCCAAGTTTTTTGAGGAATTGACCAGAAAACCACGCGGACTTATCCTGGTGACAGGTCCCACCGGCT
CAGGCAAATCAACTACACTGGCGGCGATGATTAATCATATTAATTGTAATGATTACGCACATATACTGACTGTAGAAGAC
CCCATCGAGTTTGTTCATGACAGCCAGAAATGTTTAATTAATCAGCGCGAAGTCCATCGCGATACCCATGGCTTTAATGA
AGCGTTACGCTCGGCACTACGTGAAGATCCTGATATTATTATGGTAGGTGAAATGCGCGATCTGGAAACTATCCGTTTGG
CATTAACCGCTGCTGAAACAGGTCACCTTGTTTTTGGTACTTTACATACCACTTCAGCAGCCAAAACCATCGACCGTATT
ATTGACGTCTTCCCGGCTGCAGAAAAAGACATGATTCGTTCCATGCTGTCGGAATCTTTACAGGCCGTTATTTCACAAAC
ACTGATGAAAAAAACCGGCGGTGGCCGTGTTGCTGCACATGAAATCATGGTGGGTACCTCGGCTATCAGAAACCTGATAC
GCGAAGCCAAAGTCGCACAGATGTATTCTGCCATTCAAACCGGGCGCAAGGAAGGTATGCAGACGCTGGATCAAAACCTG
AGAGAACTGGTTGATAAAGGTTTGGTTACCTCAAAAGAAGCAATGGTTAAAGCCGTTAATAAAGATAGCTTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Acinetobacter baumannii D1279779

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

78.488

99.71

0.783
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Pseudomonas aeruginosa PAK

77.326

99.71

0.771
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.681

0.675
  pilT Vibrio cholerae strain A1552

69.139

97.681

0.675
  pilT Neisseria meningitidis 8013

65.507

100

0.655
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Acinetobacter baylyi ADP1

40.058

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

39.306

100

0.394
  pilU Vibrio cholerae strain A1552

37.572

100

0.377