Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KKZ03_RS05560 Genome accession   NZ_CP076024
Coordinates   1203156..1204313 (-) Length   385 a.a.
NCBI ID   WP_243221543.1    Uniprot ID   -
Organism   Methylobacter sp. S3L5C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1198156..1209313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKZ03_RS05535 (KKZ03_05535) trpE 1198817..1200304 (+) 1488 WP_243220541.1 anthranilate synthase component I -
  KKZ03_RS05540 (KKZ03_05540) - 1200301..1200891 (+) 591 WP_243220542.1 aminodeoxychorismate/anthranilate synthase component II -
  KKZ03_RS05545 (KKZ03_05545) - 1200913..1201143 (+) 231 WP_243220543.1 DUF2442 domain-containing protein -
  KKZ03_RS05550 (KKZ03_05550) trpD 1201180..1202196 (+) 1017 WP_243220544.1 anthranilate phosphoribosyltransferase -
  KKZ03_RS05555 (KKZ03_05555) trpC 1202210..1203010 (+) 801 WP_243220545.1 indole-3-glycerol phosphate synthase TrpC -
  KKZ03_RS05560 (KKZ03_05560) pilU 1203156..1204313 (-) 1158 WP_243221543.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KKZ03_RS05565 (KKZ03_05565) pilT 1204324..1205361 (-) 1038 WP_243220546.1 type IV pilus twitching motility protein PilT Machinery gene
  KKZ03_RS05570 (KKZ03_05570) - 1205488..1206159 (+) 672 WP_243220547.1 YggS family pyridoxal phosphate-dependent enzyme -
  KKZ03_RS05575 (KKZ03_05575) - 1206172..1206876 (-) 705 WP_243220548.1 hypothetical protein -
  KKZ03_RS21975 - 1207477..1208813 (-) 1337 Protein_1105 IS4 family transposase -
  KKZ03_RS05590 (KKZ03_05590) - 1208959..1209087 (-) 129 Protein_1106 group II intron maturase-specific domain-containing protein -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 43162.47 Da        Isoelectric Point: 5.9100

>NTDB_id=571346 KKZ03_RS05560 WP_243221543.1 1203156..1204313(-) (pilU) [Methylobacter sp. S3L5C]
MNLNTLFAIMAEKKASDLFITAGRAPTIKVDNTLIEVSKTPLTAEQALEVVLSTMNPRQKTEFENTKECQYALGVKDIGR
FRISAFTQRDSAGMVMRRIENEIPDADSLFLPPVLKEIIMEKRGLILFVGATGTGKSTSLAALIKHRNQNSSGHIITIED
PIEFQHPHMGCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMQNAITFAETGHLCLSTLHANNANQALDRI
LHFFPDEMHGQLLMDLSLNLKGIVAQQLIKRADGKGRYPAVEILINTPLIKDYIRKGEIDKIKELMKNSREQGMQTFDQA
LYDLYIAGKISYEDALNSADSRNEVRLMIKLSGENITTFDDNDGMRLSATEENTFFANNAFKRPD

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=571346 KKZ03_RS05560 WP_243221543.1 1203156..1204313(-) (pilU) [Methylobacter sp. S3L5C]
ATGAATTTGAATACACTATTTGCAATAATGGCCGAGAAAAAAGCCTCTGATTTATTTATAACCGCAGGAAGGGCTCCGAC
CATAAAGGTTGATAACACTCTGATTGAGGTTTCCAAGACGCCATTGACAGCAGAGCAGGCTTTAGAGGTTGTCCTGAGTA
CGATGAACCCGCGACAAAAAACCGAGTTTGAAAATACCAAGGAGTGTCAATATGCCCTGGGTGTAAAAGATATAGGAAGG
TTTAGAATCAGCGCCTTTACCCAGCGAGATTCTGCCGGTATGGTAATGCGACGGATTGAAAATGAAATACCCGATGCTGA
TTCCTTGTTTTTGCCACCTGTTTTAAAAGAAATAATTATGGAAAAACGTGGCCTTATTCTGTTTGTAGGGGCTACAGGCA
CAGGCAAATCAACCTCATTGGCTGCCTTAATCAAGCATCGTAATCAAAACAGTAGCGGCCATATTATTACCATTGAGGAT
CCTATAGAGTTTCAGCATCCGCATATGGGTTGTATTGTCACCCAGCGTGAAGTAGGAATTGATACGGAATCTTATGAGGT
TGCGTTAAAAAACACACTACGACAGGCACCTGATGTTATTTTGATTGGTGAAATCAGGACACGGGAAACCATGCAGAATG
CCATTACTTTTGCCGAGACAGGACATTTATGCCTGTCTACATTACATGCAAACAATGCCAACCAAGCCTTGGATCGAATT
TTGCATTTCTTTCCTGATGAAATGCATGGCCAGTTATTAATGGATTTGTCATTGAATCTGAAAGGAATCGTTGCCCAACA
ATTAATCAAGCGAGCCGATGGCAAGGGTCGCTATCCCGCCGTCGAAATATTGATTAATACGCCATTGATCAAAGATTATA
TCCGTAAAGGCGAGATTGACAAGATTAAAGAGTTAATGAAAAACTCACGCGAACAAGGTATGCAAACTTTTGACCAAGCC
CTGTATGACTTGTATATCGCTGGAAAAATCAGTTATGAAGATGCGTTAAATTCAGCAGATTCAAGAAACGAAGTACGCTT
AATGATAAAACTAAGCGGTGAAAATATTACGACTTTTGACGATAATGATGGAATGCGCTTATCAGCAACAGAGGAGAATA
CATTTTTTGCCAATAATGCTTTTAAAAGACCGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.804

97.922

0.605
  pilU Acinetobacter baylyi ADP1

60.477

97.922

0.592
  pilU Vibrio cholerae strain A1552

56.857

90.909

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.379

90.39

0.374
  pilT Legionella pneumophila strain Lp02

40.816

89.091

0.364
  pilT Legionella pneumophila strain ERS1305867

40.816

89.091

0.364