Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KI617_RS18550 Genome accession   NZ_CP075191
Coordinates   3869214..3870257 (-) Length   347 a.a.
NCBI ID   WP_226448844.1    Uniprot ID   -
Organism   Ferribacterium limneticum strain 128     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3864214..3875257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI617_RS18525 (KI617_18470) - 3864237..3864680 (+) 444 WP_226448836.1 cytochrome c5 family protein -
  KI617_RS18530 (KI617_18475) - 3864871..3866862 (-) 1992 WP_226448838.1 UvrD-helicase domain-containing protein -
  KI617_RS18535 (KI617_18480) - 3866971..3867288 (-) 318 WP_226448840.1 hypothetical protein -
  KI617_RS18540 (KI617_18485) - 3867339..3868025 (-) 687 WP_226448842.1 DUF4197 domain-containing protein -
  KI617_RS18545 (KI617_18490) pilU 3868044..3869180 (-) 1137 WP_319004126.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI617_RS18550 (KI617_18495) pilT 3869214..3870257 (-) 1044 WP_226448844.1 type IV pilus twitching motility protein PilT Machinery gene
  KI617_RS18555 (KI617_18500) - 3870314..3871003 (+) 690 WP_226448846.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI617_RS18560 (KI617_18505) proC 3871000..3871812 (+) 813 WP_226448848.1 pyrroline-5-carboxylate reductase -
  KI617_RS18565 (KI617_18510) - 3871812..3872345 (+) 534 WP_226448850.1 YggT family protein -
  KI617_RS18570 (KI617_18515) - 3872342..3872632 (+) 291 WP_226448851.1 DUF167 domain-containing protein -
  KI617_RS18575 (KI617_18520) - 3872681..3872962 (-) 282 WP_226448853.1 helix-hairpin-helix domain-containing protein -
  KI617_RS18580 (KI617_18525) - 3873082..3874332 (-) 1251 WP_226448855.1 dihydroorotase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38542.18 Da        Isoelectric Point: 6.8848

>NTDB_id=568584 KI617_RS18550 WP_226448844.1 3869214..3870257(-) (pilT) [Ferribacterium limneticum strain 128]
MDITELLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINLPAMEHKDVHGMVYDIMNDGQRKIYEETLECDFSFEIPNLAR
FRVNAFNQQRGAAAVFRTIPSKVLTLEELNCPKIFKEISEYPRGIVLVTGPTGSGKSTTLAAMVNHVNENEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQNFGMQTLDQN
LIDMVRRNVVSSNEARYKAANKDAFPA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=568584 KI617_RS18550 WP_226448844.1 3869214..3870257(-) (pilT) [Ferribacterium limneticum strain 128]
ATGGACATCACCGAACTGCTGGCCTTCAGTGTCAAGAACAAGGCTTCAGACCTTCACTTGTCGTCCGGGTTGCCGCCCAT
GATCCGCGTGCACGGCGACGTCCGTCGAATCAACTTGCCGGCCATGGAGCACAAGGATGTGCACGGCATGGTGTACGACA
TCATGAACGACGGTCAGCGCAAGATTTACGAAGAAACGCTGGAGTGCGACTTCTCGTTCGAAATCCCCAACCTGGCGCGT
TTCCGGGTCAACGCCTTCAACCAGCAACGCGGCGCCGCCGCGGTTTTCCGGACCATTCCGTCCAAGGTGCTGACGCTTGA
AGAGTTGAACTGCCCGAAAATTTTCAAGGAAATTTCGGAGTATCCGCGCGGTATCGTGCTGGTTACCGGGCCGACCGGTT
CCGGCAAATCGACGACGTTGGCGGCGATGGTCAATCACGTTAATGAAAACGAGTATGGCCACATCCTGACGGTCGAAGAT
CCGATCGAATTCGTCCATGAGTCGAAGAAGTGTCTGATCAACCAGCGCGAAGTCGGGCCACATACCCTGTCTTTCTCCAA
CGCCCTGCGTTCGGCGCTGCGTGAGGATCCGGACGTGATCCTGGTCGGTGAAATGCGCGATCTGGAGACCATTCGCCTGG
CGCTGACTGCGGCTGAAACCGGTCACCTGGTTTTCGGTACCCTGCATACGTCGTCCGCAGCCAAGACGGTCGACCGCGTG
GTTGACGTTTTCCCGGCCGCAGAAAAAGAAATGGTTCGCTCGATGTTGTCCGAGTCTTTGCGCGCCGTCATTTCGCAGAC
TTTGCTCAAGACCAAAGACGGTAGCGGGCGGGTCGCGGCGCACGAAATCATGATCGGCACGCCGGCCATTCGTAACCTGA
TTCGTGAAAACAAGGTCGCCCAGATGTATTCGGCCATCCAGACCGGTCAGAATTTTGGCATGCAGACGCTGGATCAGAAT
CTGATCGACATGGTGCGGCGCAATGTGGTGTCGAGCAACGAGGCTCGCTACAAGGCGGCCAACAAGGACGCATTCCCGGC
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.13

99.424

0.787
  pilT Acinetobacter baumannii strain A118

79.13

99.424

0.787
  pilT Acinetobacter nosocomialis M2

79.13

99.424

0.787
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Neisseria meningitidis 8013

72.754

99.424

0.723
  pilT Neisseria gonorrhoeae MS11

72.464

99.424

0.72
  pilT Legionella pneumophila strain Lp02

72.174

99.424

0.718
  pilT Legionella pneumophila strain ERS1305867

72.174

99.424

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.527

97.406

0.677
  pilT Vibrio cholerae strain A1552

69.527

97.406

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.233

98.847

0.398
  pilU Vibrio cholerae strain A1552

40.413

97.695

0.395
  pilU Acinetobacter baylyi ADP1

40.476

96.83

0.392