Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KI617_RS18545 Genome accession   NZ_CP075191
Coordinates   3868044..3869180 (-) Length   378 a.a.
NCBI ID   WP_319004126.1    Uniprot ID   -
Organism   Ferribacterium limneticum strain 128     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3863044..3874180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI617_RS18520 (KI617_18465) - 3863740..3863922 (+) 183 WP_226448834.1 hypothetical protein -
  KI617_RS18525 (KI617_18470) - 3864237..3864680 (+) 444 WP_226448836.1 cytochrome c5 family protein -
  KI617_RS18530 (KI617_18475) - 3864871..3866862 (-) 1992 WP_226448838.1 UvrD-helicase domain-containing protein -
  KI617_RS18535 (KI617_18480) - 3866971..3867288 (-) 318 WP_226448840.1 hypothetical protein -
  KI617_RS18540 (KI617_18485) - 3867339..3868025 (-) 687 WP_226448842.1 DUF4197 domain-containing protein -
  KI617_RS18545 (KI617_18490) pilU 3868044..3869180 (-) 1137 WP_319004126.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI617_RS18550 (KI617_18495) pilT 3869214..3870257 (-) 1044 WP_226448844.1 type IV pilus twitching motility protein PilT Machinery gene
  KI617_RS18555 (KI617_18500) - 3870314..3871003 (+) 690 WP_226448846.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI617_RS18560 (KI617_18505) proC 3871000..3871812 (+) 813 WP_226448848.1 pyrroline-5-carboxylate reductase -
  KI617_RS18565 (KI617_18510) - 3871812..3872345 (+) 534 WP_226448850.1 YggT family protein -
  KI617_RS18570 (KI617_18515) - 3872342..3872632 (+) 291 WP_226448851.1 DUF167 domain-containing protein -
  KI617_RS18575 (KI617_18520) - 3872681..3872962 (-) 282 WP_226448853.1 helix-hairpin-helix domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42263.70 Da        Isoelectric Point: 6.6642

>NTDB_id=568583 KI617_RS18545 WP_319004126.1 3868044..3869180(-) (pilU) [Ferribacterium limneticum strain 128]
MERDQAMKFMHDLLRLMSQKKGSDLFITAGFPPAIKIDGKITPVSNQILTPQHTAELARSIMNDRQASEFEATKECNFAI
SPAGIGRFRVNALVQQGRVGVVCRTINMSIPTLDELQLPPVLKDLAMTKRGLIIFVGGTGTGKTTSLAALVDYRNENSYG
HIITIEDPIEYVHDHKNCIVTQREVGVDTDDWGPALKNTLRQAPDVILMGEIRDRNTMDYAIAFAETGHLALATLHANSA
NQAIDRIINFFPEERRQQLLMDLSLNLRAMVSQRLIPKKDGKGRMAAIEVMLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEAGKITYEDALRNADSLNDLRLQIKLHGKESKDRDLTTGIGHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=568583 KI617_RS18545 WP_319004126.1 3868044..3869180(-) (pilU) [Ferribacterium limneticum strain 128]
ATGGAACGCGATCAAGCAATGAAATTCATGCACGATCTTTTGCGCCTGATGTCGCAGAAGAAAGGCTCGGACCTTTTCAT
CACGGCAGGTTTCCCGCCGGCCATCAAGATCGACGGCAAGATCACGCCCGTCTCGAATCAGATTCTGACGCCGCAGCATA
CGGCCGAACTGGCGCGCTCGATCATGAACGATCGCCAGGCCTCCGAGTTCGAAGCGACCAAGGAATGTAACTTTGCGATT
TCGCCGGCCGGCATCGGACGCTTCCGGGTCAACGCGCTGGTCCAGCAGGGTCGGGTCGGCGTTGTTTGCCGGACGATCAA
CATGAGCATCCCGACATTGGACGAATTGCAGTTGCCGCCGGTCTTGAAGGATCTGGCGATGACCAAGCGCGGCCTGATCA
TCTTCGTCGGCGGTACCGGCACCGGCAAGACGACCTCGCTGGCGGCGTTGGTCGATTACCGTAACGAAAACTCCTACGGC
CACATCATCACGATCGAAGACCCGATTGAATATGTGCACGATCACAAGAACTGCATCGTCACGCAGCGCGAGGTCGGCGT
TGACACCGACGACTGGGGCCCGGCCCTGAAGAACACGCTACGTCAGGCGCCTGACGTCATCCTGATGGGCGAAATCCGTG
ATCGCAACACTATGGACTACGCCATCGCCTTCGCCGAAACCGGCCACCTGGCGTTGGCAACCCTGCACGCCAACAGCGCC
AACCAGGCCATTGACCGGATCATCAACTTCTTCCCGGAGGAGCGTCGCCAGCAACTGCTGATGGACCTTTCGCTGAACCT
GCGTGCCATGGTTTCGCAGCGCCTGATTCCGAAAAAGGATGGCAAGGGCCGCATGGCAGCCATTGAAGTGATGCTCAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGTGAGGTACACGAGATCAAGGAAATCATGAAGAAATCCCGTGAACTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTTTACGAGGCCGGCAAGATTACCTACGAGGACGCCCTGCGCAACGCCGA
TTCACTGAACGACCTGCGCCTGCAGATCAAGCTGCACGGCAAGGAATCCAAGGATCGTGACCTGACCACCGGTATCGGTC
ATCTGGATATTGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.56

96.296

0.622
  pilU Acinetobacter baylyi ADP1

60.446

94.974

0.574
  pilU Vibrio cholerae strain A1552

54.252

90.212

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

88.624

0.384
  pilT Pseudomonas aeruginosa PAK

42.388

88.624

0.376
  pilT Acinetobacter baylyi ADP1

41.543

89.153

0.37
  pilT Legionella pneumophila strain Lp02

42.296

87.566

0.37
  pilT Legionella pneumophila strain ERS1305867

42.296

87.566

0.37
  pilT Pseudomonas stutzeri DSM 10701

40.896

88.624

0.362
  pilT Acinetobacter baumannii D1279779

41.39

87.566

0.362
  pilT Acinetobacter nosocomialis M2

41.39

87.566

0.362
  pilT Acinetobacter baumannii strain A118

41.39

87.566

0.362