Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KI610_RS18335 Genome accession   NZ_CP075189
Coordinates   3823304..3824347 (-) Length   347 a.a.
NCBI ID   WP_226496379.1    Uniprot ID   -
Organism   Ferribacterium limneticum strain 77     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3818304..3829347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI610_RS18310 (KI610_18225) - 3818476..3818796 (-) 321 WP_226496375.1 hypothetical protein -
  KI610_RS18315 (KI610_18230) - 3818936..3820927 (-) 1992 WP_226496376.1 UvrD-helicase domain-containing protein -
  KI610_RS18320 (KI610_18235) - 3821065..3821382 (-) 318 WP_226496377.1 hypothetical protein -
  KI610_RS18325 (KI610_18240) - 3821427..3822107 (-) 681 WP_226496378.1 DUF4197 domain-containing protein -
  KI610_RS18330 (KI610_18245) pilU 3822133..3823269 (-) 1137 WP_310491598.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI610_RS18335 (KI610_18250) pilT 3823304..3824347 (-) 1044 WP_226496379.1 type IV pilus twitching motility protein PilT Machinery gene
  KI610_RS18340 (KI610_18255) - 3824404..3825093 (+) 690 WP_226496380.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI610_RS18345 (KI610_18260) proC 3825090..3825902 (+) 813 WP_226496381.1 pyrroline-5-carboxylate reductase -
  KI610_RS18350 (KI610_18265) - 3825902..3826435 (+) 534 WP_226496382.1 YggT family protein -
  KI610_RS18355 (KI610_18270) - 3826432..3826725 (+) 294 WP_226496383.1 DUF167 domain-containing protein -
  KI610_RS18360 (KI610_18275) ureG 3826735..3827340 (-) 606 WP_226496384.1 urease accessory protein UreG -
  KI610_RS18365 (KI610_18280) - 3827337..3827696 (-) 360 WP_226496385.1 endonuclease domain-containing protein -
  KI610_RS18370 (KI610_18285) - 3827791..3828468 (-) 678 WP_226496386.1 urease accessory protein UreF -
  KI610_RS18375 (KI610_18290) - 3828483..3828689 (-) 207 WP_226402565.1 4-oxalocrotonate tautomerase family protein -
  KI610_RS18380 (KI610_18295) ureE 3828707..3829306 (-) 600 WP_226496387.1 urease accessory protein UreE -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38476.13 Da        Isoelectric Point: 6.9272

>NTDB_id=568537 KI610_RS18335 WP_226496379.1 3823304..3824347(-) (pilT) [Ferribacterium limneticum strain 77]
MDITELLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINLPAMEHKDVHGMVYDIMNDGQRKVYEETLECDFSFEIPNLAR
FRVNAFNQHRGAGAVFRTIPSKVLTLEELNCPKIFKEISEYPRGIVLCTGPTGSGKSTTLAAMVNHVNENDYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFANALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNFGMQTLDQN
LIELVRRNVVSSAEARFRAANKDAFPA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=568537 KI610_RS18335 WP_226496379.1 3823304..3824347(-) (pilT) [Ferribacterium limneticum strain 77]
ATGGACATCACCGAACTGCTGGCTTTCAGCGTCAAGAACAAAGCCTCCGACCTCCACCTGTCCTCCGGCCTGCCGCCGAT
GATCCGCGTGCATGGCGATGTCAGGCGCATCAACCTGCCAGCCATGGAACACAAGGACGTTCATGGCATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGGTTTATGAAGAGACGCTGGAATGCGACTTTTCCTTCGAAATCCCCAATCTGGCGCGT
TTCCGGGTCAATGCCTTCAATCAGCATCGCGGCGCCGGCGCCGTCTTCCGGACCATTCCGTCCAAGGTGCTGACGCTCGA
AGAGCTCAACTGCCCGAAGATTTTCAAGGAGATTTCTGAGTATCCGCGCGGCATCGTGTTGTGTACCGGGCCGACCGGTT
CGGGCAAATCGACGACGCTGGCGGCGATGGTCAATCACGTTAACGAAAACGATTACGGCCATATCCTGACCGTCGAGGAT
CCGATCGAATTCGTCCATGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGCATACGCTGTCCTTCGCCAA
CGCCCTGCGCTCGGCGCTGCGTGAGGATCCGGACGTCATCCTGGTCGGCGAAATGCGCGATCTGGAAACCATCCGTCTGG
CCCTCACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACTTCGTCGGCCGCCAAAACGGTGGACCGCGTG
GTCGACGTCTTCCCGGCGGCGGAAAAGGAAATGGTCCGCTCCATGCTGTCCGAATCGCTGCGCGCCGTCATCTCGCAGAC
GCTGCTCAAGACCAAGGACGGTAGTGGCCGGGTCGCGGCGCACGAAATCATGATCGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAAAACAAGATCGCCCAGATGTATTCGGCCATCCAGACCGGCCAGAATTTCGGCATGCAGACGCTCGACCAGAAT
CTGATCGAACTGGTCCGGCGCAACGTGGTGTCGAGCGCCGAAGCGCGCTTCCGCGCCGCCAACAAGGATGCTTTCCCGGC
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.13

99.424

0.787
  pilT Acinetobacter baumannii D1279779

79.13

99.424

0.787
  pilT Acinetobacter baumannii strain A118

79.13

99.424

0.787
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Neisseria meningitidis 8013

73.043

99.424

0.726
  pilT Neisseria gonorrhoeae MS11

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

72.464

99.424

0.72
  pilT Legionella pneumophila strain ERS1305867

72.464

99.424

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

96.83

0.671
  pilT Vibrio cholerae strain A1552

69.345

96.83

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Pseudomonas stutzeri DSM 10701

40.525

98.847

0.401
  pilU Acinetobacter baylyi ADP1

41.369

96.83

0.401
  pilU Vibrio cholerae strain A1552

40.896

96.542

0.395