Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KI610_RS18330 Genome accession   NZ_CP075189
Coordinates   3822133..3823269 (-) Length   378 a.a.
NCBI ID   WP_310491598.1    Uniprot ID   -
Organism   Ferribacterium limneticum strain 77     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3817133..3828269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI610_RS18300 (KI610_18215) - 3817406..3817588 (+) 183 WP_226496373.1 hypothetical protein -
  KI610_RS18305 (KI610_18220) - 3817902..3818345 (+) 444 WP_226496374.1 cytochrome c5 family protein -
  KI610_RS18310 (KI610_18225) - 3818476..3818796 (-) 321 WP_226496375.1 hypothetical protein -
  KI610_RS18315 (KI610_18230) - 3818936..3820927 (-) 1992 WP_226496376.1 UvrD-helicase domain-containing protein -
  KI610_RS18320 (KI610_18235) - 3821065..3821382 (-) 318 WP_226496377.1 hypothetical protein -
  KI610_RS18325 (KI610_18240) - 3821427..3822107 (-) 681 WP_226496378.1 DUF4197 domain-containing protein -
  KI610_RS18330 (KI610_18245) pilU 3822133..3823269 (-) 1137 WP_310491598.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI610_RS18335 (KI610_18250) pilT 3823304..3824347 (-) 1044 WP_226496379.1 type IV pilus twitching motility protein PilT Machinery gene
  KI610_RS18340 (KI610_18255) - 3824404..3825093 (+) 690 WP_226496380.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI610_RS18345 (KI610_18260) proC 3825090..3825902 (+) 813 WP_226496381.1 pyrroline-5-carboxylate reductase -
  KI610_RS18350 (KI610_18265) - 3825902..3826435 (+) 534 WP_226496382.1 YggT family protein -
  KI610_RS18355 (KI610_18270) - 3826432..3826725 (+) 294 WP_226496383.1 DUF167 domain-containing protein -
  KI610_RS18360 (KI610_18275) ureG 3826735..3827340 (-) 606 WP_226496384.1 urease accessory protein UreG -
  KI610_RS18365 (KI610_18280) - 3827337..3827696 (-) 360 WP_226496385.1 endonuclease domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42258.71 Da        Isoelectric Point: 6.6644

>NTDB_id=568536 KI610_RS18330 WP_310491598.1 3822133..3823269(-) (pilU) [Ferribacterium limneticum strain 77]
MERDQAMKFMHDLLRLMSQKKGSDLFITAGFPPAIKIDGKITPVSNQILTPQHTAELARSIMNDRQASEFEATKECNFAI
SPAGIGRFRVNALVQQGRVGVVCRTINMSIPTLDELLLPPVLKDLAMTKRGLIIFVGGTGTGKTTSLAALVDYRNENSYG
HIITIEDPIEYVHEHKNCIVTQREVGVDTDDWGPALKNTLRQAPDVILMGEIRDRNTMDYAIAFAETGHLALATLHANSA
NQAIDRIINFFPEERRQQLLMDLSLNLRALVSQRLIPKKDGRGRMAAIEVMLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEAGKITYEDALRNADSLNDLRLQIKLHGKESKDRDLSTGIGHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=568536 KI610_RS18330 WP_310491598.1 3822133..3823269(-) (pilU) [Ferribacterium limneticum strain 77]
ATGGAACGCGACCAGGCAATGAAATTCATGCACGATCTTTTGCGTTTGATGTCGCAAAAGAAAGGCTCCGACCTTTTCAT
CACCGCCGGCTTTCCGCCAGCCATCAAGATCGACGGCAAGATCACGCCGGTCTCGAACCAGATTCTGACGCCGCAGCACA
CGGCCGAACTGGCGCGTTCGATTATGAATGACCGCCAGGCTTCGGAGTTCGAAGCAACGAAGGAATGCAATTTCGCCATT
TCGCCGGCCGGCATCGGCCGTTTCCGGGTCAATGCCCTGGTTCAGCAGGGTCGTGTCGGTGTCGTCTGTCGGACGATCAA
CATGAGCATTCCGACGCTGGATGAGCTGCTGTTGCCCCCGGTGCTCAAGGATCTGGCGATGACCAAGCGCGGCCTGATCA
TCTTCGTCGGCGGCACCGGTACCGGCAAGACGACGTCGCTGGCGGCCCTCGTCGATTACCGCAACGAGAATTCGTATGGC
CACATCATTACCATCGAAGACCCGATCGAATACGTGCACGAGCACAAGAACTGTATCGTCACCCAGCGTGAAGTCGGCGT
TGATACCGACGACTGGGGCCCGGCGCTCAAGAACACACTGCGCCAGGCGCCGGACGTTATCCTGATGGGCGAAATCCGCG
ACCGCAATACCATGGACTACGCCATCGCCTTCGCCGAAACCGGCCACCTGGCATTGGCGACGCTGCACGCCAACAGCGCC
AACCAGGCCATCGACCGGATCATCAACTTCTTCCCGGAAGAGCGTCGCCAGCAACTGCTCATGGACCTCTCGCTGAACCT
GCGCGCGCTCGTTTCGCAGCGCCTGATTCCGAAAAAAGATGGTCGCGGGCGGATGGCGGCGATCGAGGTCATGCTCAATT
CGCCGCTGATTTCGGACCTGATCTTCAAGGGCGAAGTGCACGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTCGGC
ATGCAGACTTTCGACCAGGCGCTGTTCGACCTCTACGAAGCCGGCAAGATCACCTACGAAGATGCGCTACGCAACGCCGA
CTCGCTCAACGACCTGCGCCTGCAGATCAAGCTGCATGGCAAGGAGTCGAAGGACCGCGATCTATCGACCGGCATCGGTC
ACCTCGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.011

96.296

0.616
  pilU Acinetobacter baylyi ADP1

60.724

94.974

0.577
  pilU Vibrio cholerae strain A1552

54.252

90.212

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

88.624

0.384
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Acinetobacter baylyi ADP1

41.84

89.153

0.373
  pilT Legionella pneumophila strain ERS1305867

42.296

87.566

0.37
  pilT Legionella pneumophila strain Lp02

42.296

87.566

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.194

88.624

0.365
  pilT Acinetobacter baumannii D1279779

41.39

87.566

0.362
  pilT Acinetobacter baumannii strain A118

41.39

87.566

0.362
  pilT Acinetobacter nosocomialis M2

41.39

87.566

0.362