Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KI614_RS14950 Genome accession   NZ_CP075187
Coordinates   3172728..3173771 (-) Length   347 a.a.
NCBI ID   WP_203467827.1    Uniprot ID   -
Organism   Dechloromonas denitrificans strain 98     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3167728..3178771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI614_RS14925 (KI614_14870) - 3167920..3168255 (-) 336 WP_226406645.1 amine oxidase -
  KI614_RS14930 (KI614_14875) - 3168394..3170385 (-) 1992 WP_203467823.1 UvrD-helicase domain-containing protein -
  KI614_RS14935 (KI614_14880) - 3170484..3170801 (-) 318 WP_226406646.1 hypothetical protein -
  KI614_RS14940 (KI614_14885) - 3170850..3171536 (-) 687 WP_226406647.1 DUF4197 domain-containing protein -
  KI614_RS14945 (KI614_14890) pilU 3171561..3172697 (-) 1137 WP_319002666.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI614_RS14950 (KI614_14895) pilT 3172728..3173771 (-) 1044 WP_203467827.1 type IV pilus twitching motility protein PilT Machinery gene
  KI614_RS14955 (KI614_14900) - 3173825..3174514 (+) 690 WP_226406648.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI614_RS14960 (KI614_14905) proC 3174511..3175323 (+) 813 WP_226406649.1 pyrroline-5-carboxylate reductase -
  KI614_RS14965 (KI614_14910) - 3175326..3175859 (+) 534 WP_203467830.1 YggT family protein -
  KI614_RS14970 (KI614_14915) - 3175867..3176160 (+) 294 WP_226406650.1 DUF167 domain-containing protein -
  KI614_RS14975 (KI614_14920) - 3176212..3176550 (-) 339 WP_264178074.1 ComEA family DNA-binding protein -
  KI614_RS14980 (KI614_14925) - 3176690..3177943 (-) 1254 WP_226406652.1 dihydroorotase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38422.10 Da        Isoelectric Point: 7.1069

>NTDB_id=568491 KI614_RS14950 WP_203467827.1 3172728..3173771(-) (pilT) [Dechloromonas denitrificans strain 98]
MDITELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHGMVYDIMNDGQRKVYEETLECDFSFEIPNLAR
FRVNAFNQQRGAGAVFRTIPTKVMTLEELNCPKIFREISEYPRGIVLVTGPTGSGKSTTLAAMVNHVNENHYGHILTVED
PIEFVHDAKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGAGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQNVGMQTLDQN
LLDMVRRGIVSSNDARNKASNKDAFPA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=568491 KI614_RS14950 WP_203467827.1 3172728..3173771(-) (pilT) [Dechloromonas denitrificans strain 98]
ATGGACATTACCGAACTGCTGGCTTTCAGCGTCAAGAACAAGGCTTCCGACTTGCACCTGTCGGCCGGCCTGCCGCCCAT
GATCCGGGTGCACGGCGATGTTCGGCGGATCAACCTGCCGCCGATGGAGCACAAAGATGTTCATGGCATGGTCTATGACA
TCATGAATGACGGCCAGCGCAAGGTTTATGAAGAAACGCTGGAGTGCGACTTCTCCTTCGAAATTCCCAACCTGGCGCGT
TTCCGGGTCAATGCCTTCAACCAGCAGCGGGGTGCCGGCGCCGTTTTCCGGACCATTCCGACCAAGGTCATGACGCTGGA
AGAGCTCAACTGTCCGAAGATTTTCCGCGAAATTTCCGAGTATCCGCGCGGTATCGTACTGGTCACCGGGCCGACCGGCT
CGGGCAAGTCGACGACGCTGGCGGCGATGGTCAATCACGTCAATGAGAACCACTATGGTCATATCCTGACGGTCGAGGAC
CCGATCGAATTTGTGCACGATGCCAAGAAGTGCCTGATCAACCAGCGTGAAGTCGGGCCGCATACCCTGTCTTTCTCGAA
CGCCCTGCGCTCGGCGCTGCGCGAAGATCCGGACGTGATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGCCTGG
CTCTGACGGCGGCCGAAACCGGCCACCTGGTATTCGGCACGCTGCACACTTCTTCGGCTGCCAAGACGGTCGACCGTATC
GTTGACGTTTTCCCGGCGGCAGAAAAGGAAATGGTTCGTTCGATGCTGTCCGAGTCGCTGCGCGCCGTCATTTCGCAGAC
CCTGCTCAAGACCAAGGATGGCGCCGGTCGCGTGGCCGCCCATGAAATCATGATCGGCACGCCGGCCATCCGTAACCTGA
TTCGTGAAAACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCCAGAATGTCGGCATGCAGACGCTGGATCAGAAT
CTGCTGGACATGGTTCGGCGCGGTATTGTTTCGAGCAACGATGCCCGCAACAAGGCATCGAACAAGGATGCTTTCCCGGC
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.971

99.424

0.775
  pilT Acinetobacter baumannii strain A118

77.971

99.424

0.775
  pilT Acinetobacter nosocomialis M2

77.971

99.424

0.775
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Neisseria meningitidis 8013

71.594

99.424

0.712
  pilT Legionella pneumophila strain Lp02

71.594

99.424

0.712
  pilT Legionella pneumophila strain ERS1305867

71.594

99.424

0.712
  pilT Neisseria gonorrhoeae MS11

71.304

99.424

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.118

97.406

0.683
  pilT Vibrio cholerae strain A1552

70.118

97.406

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Acinetobacter baylyi ADP1

40.936

98.559

0.403
  pilU Vibrio cholerae strain A1552

41.056

98.271

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403