Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KI614_RS14945 Genome accession   NZ_CP075187
Coordinates   3171561..3172697 (-) Length   378 a.a.
NCBI ID   WP_319002666.1    Uniprot ID   -
Organism   Dechloromonas denitrificans strain 98     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3166561..3177697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI614_RS14915 (KI614_14860) - 3166930..3167112 (+) 183 WP_203467820.1 hypothetical protein -
  KI614_RS14920 (KI614_14865) - 3167339..3167788 (+) 450 WP_226406644.1 c-type cytochrome -
  KI614_RS14925 (KI614_14870) - 3167920..3168255 (-) 336 WP_226406645.1 amine oxidase -
  KI614_RS14930 (KI614_14875) - 3168394..3170385 (-) 1992 WP_203467823.1 UvrD-helicase domain-containing protein -
  KI614_RS14935 (KI614_14880) - 3170484..3170801 (-) 318 WP_226406646.1 hypothetical protein -
  KI614_RS14940 (KI614_14885) - 3170850..3171536 (-) 687 WP_226406647.1 DUF4197 domain-containing protein -
  KI614_RS14945 (KI614_14890) pilU 3171561..3172697 (-) 1137 WP_319002666.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI614_RS14950 (KI614_14895) pilT 3172728..3173771 (-) 1044 WP_203467827.1 type IV pilus twitching motility protein PilT Machinery gene
  KI614_RS14955 (KI614_14900) - 3173825..3174514 (+) 690 WP_226406648.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI614_RS14960 (KI614_14905) proC 3174511..3175323 (+) 813 WP_226406649.1 pyrroline-5-carboxylate reductase -
  KI614_RS14965 (KI614_14910) - 3175326..3175859 (+) 534 WP_203467830.1 YggT family protein -
  KI614_RS14970 (KI614_14915) - 3175867..3176160 (+) 294 WP_226406650.1 DUF167 domain-containing protein -
  KI614_RS14975 (KI614_14920) - 3176212..3176550 (-) 339 WP_264178074.1 ComEA family DNA-binding protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42217.61 Da        Isoelectric Point: 6.6644

>NTDB_id=568490 KI614_RS14945 WP_319002666.1 3171561..3172697(-) (pilU) [Dechloromonas denitrificans strain 98]
MERDQAMKFMHDLLRLMSQKKGSDLFIAAGFAPAIKIDGKVTPVSNQVLTAQHTAELARSIMNDRQAAEFEATKECNFAI
SPSGIGRFRVNALVQQGRVGVVCRTINMTIPTLDELHMPPVLKDLAMSKRGLIIFVGGTGTGKTTSLAALVDYRNENSFG
HIITIEDPIEYVHEHKNCIVTQREVGVDTDDWGPALKNTLRQAPDVILMGEIRDRNTMDYAIAFAETGHLALATLHANSA
NQAIDRIINFFPEERRQQLLMDLSLNMRAMVSQRLLPKKDGKGRVAAIEVMLNSPLIADLIFRGEVHEIKEIMKKSRELG
MQTFDQALFDLYEAGKISYEDALRNADSLNDLRLQIKLYGKESKDRDLSSGLNHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=568490 KI614_RS14945 WP_319002666.1 3171561..3172697(-) (pilU) [Dechloromonas denitrificans strain 98]
ATGGAACGCGATCAGGCAATGAAGTTCATGCACGATCTTTTGCGCCTGATGTCGCAAAAGAAAGGCTCCGATTTGTTCAT
CGCCGCCGGTTTTGCACCAGCGATCAAGATCGATGGCAAGGTGACGCCGGTGTCGAACCAGGTATTGACGGCACAACACA
CCGCCGAGCTGGCCCGCTCGATCATGAACGACCGCCAGGCGGCCGAGTTCGAGGCGACCAAGGAGTGCAATTTCGCCATT
TCGCCTTCCGGTATCGGGCGCTTCCGGGTCAATGCGCTGGTGCAGCAAGGTCGCGTCGGTGTCGTGTGCCGGACGATCAA
CATGACGATTCCGACACTCGATGAGTTGCACATGCCGCCGGTGCTCAAGGATCTGGCGATGAGCAAGCGCGGCCTGATCA
TCTTCGTCGGCGGCACCGGTACCGGCAAGACGACCTCGCTGGCTGCCTTGGTCGATTACCGCAACGAGAATTCCTTCGGC
CACATCATCACCATCGAAGACCCGATCGAATATGTGCACGAGCACAAGAACTGCATCGTGACGCAGCGCGAAGTCGGCGT
TGATACTGACGACTGGGGGCCGGCCTTGAAGAACACCCTGCGCCAGGCACCGGATGTCATCCTGATGGGCGAAATCCGCG
ACCGCAACACGATGGATTACGCCATCGCCTTCGCCGAAACCGGCCACCTGGCGCTGGCTACGTTGCACGCCAACAGCGCC
AATCAGGCGATCGACCGGATCATCAATTTCTTCCCCGAAGAGCGTCGCCAGCAATTGCTGATGGATCTGTCGCTCAATAT
GCGGGCCATGGTTTCCCAGCGCCTGCTGCCCAAGAAAGACGGCAAGGGGCGGGTTGCCGCGATCGAGGTGATGCTCAATT
CGCCGCTGATCGCCGATCTGATCTTCCGTGGCGAGGTGCATGAGATCAAGGAAATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGATCTCTACGAAGCCGGCAAGATCAGCTATGAAGATGCATTGCGCAACGCCGA
TTCGCTCAATGACCTGCGCTTGCAGATCAAGTTGTATGGCAAGGAGTCGAAGGATCGTGACCTGAGTAGCGGTCTGAACC
ATCTCGATATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.363

96.296

0.601
  pilU Acinetobacter baylyi ADP1

59.889

94.974

0.569
  pilU Vibrio cholerae strain A1552

55.132

90.212

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.624

0.386
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Acinetobacter baylyi ADP1

41.543

89.153

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.624

0.37
  pilT Legionella pneumophila strain ERS1305867

41.692

87.566

0.365
  pilT Legionella pneumophila strain Lp02

41.692

87.566

0.365
  pilT Acinetobacter nosocomialis M2

41.39

87.566

0.362
  pilT Acinetobacter baumannii D1279779

41.39

87.566

0.362
  pilT Acinetobacter baumannii strain A118

41.39

87.566

0.362