Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AZKH_RS19080 Genome accession   NC_020516
Coordinates   4272342..4273382 (+) Length   346 a.a.
NCBI ID   WP_015437435.1    Uniprot ID   H0PT91
Organism   Azoarcus sp. KH32C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4267342..4278382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZKH_RS19050 (AZKH_3843) - 4267537..4268511 (+) 975 WP_015437429.1 aspartate carbamoyltransferase catalytic subunit -
  AZKH_RS19055 (AZKH_3844) - 4268511..4269791 (+) 1281 WP_015437430.1 dihydroorotase -
  AZKH_RS19060 (AZKH_3845) - 4269828..4270127 (-) 300 WP_015437431.1 DUF167 family protein -
  AZKH_RS19065 (AZKH_3846) - 4270124..4270708 (-) 585 WP_015437432.1 YggT family protein -
  AZKH_RS19070 (AZKH_3847) proC 4270715..4271527 (-) 813 WP_041657589.1 pyrroline-5-carboxylate reductase -
  AZKH_RS19075 (AZKH_3848) - 4271536..4272237 (-) 702 WP_041656400.1 YggS family pyridoxal phosphate-dependent enzyme -
  AZKH_RS19080 (AZKH_3849) pilT 4272342..4273382 (+) 1041 WP_015437435.1 type IV pilus twitching motility protein PilT Machinery gene
  AZKH_RS19085 (AZKH_3850) pilU 4273505..4274638 (+) 1134 WP_015437436.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AZKH_RS19090 (AZKH_3851) - 4274745..4275905 (-) 1161 WP_015437437.1 class I SAM-dependent RNA methyltransferase -
  AZKH_RS19095 (AZKH_3852) - 4275932..4276342 (-) 411 WP_015437438.1 CopD family protein -
  AZKH_RS19100 (AZKH_3853) - 4276430..4277320 (-) 891 WP_041656401.1 SDR family oxidoreductase -
  AZKH_RS19105 (AZKH_3854) - 4277342..4278367 (+) 1026 WP_041656403.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38355.93 Da        Isoelectric Point: 7.0291

>NTDB_id=56722 AZKH_RS19080 WP_015437435.1 4272342..4273382(+) (pilT) [Azoarcus sp. KH32C]
MDITELLAFAVKNKASDLHLSSGLPPMIRVHGDVRRINLPPMEHKDVHSMVYDIMNDAQRKQYEEVLECDFSFAVPNLAR
FRVNAFNQNRGAAAVFRTIPSKVLSLEELNAPKIFKEISNQPRGIVLVTGPTGSGKSTTLAAMIDHVNDNEYGHILTIED
PIEFVHESKRCLINQREVARDTLSFNNALRSALREDPDVILVGELRDLETIRLALTGAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVIAQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMQTLDQN
LADLVRRNIVSAGEAKQKAQNKDNFA

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=56722 AZKH_RS19080 WP_015437435.1 4272342..4273382(+) (pilT) [Azoarcus sp. KH32C]
ATGGATATCACCGAACTGCTCGCCTTCGCGGTCAAGAACAAGGCCTCCGACCTGCACCTGTCCTCCGGCCTTCCGCCGAT
GATCCGCGTACATGGCGACGTGCGGCGCATCAACCTGCCTCCGATGGAACACAAGGACGTCCATTCCATGGTGTATGACA
TCATGAATGACGCCCAGCGCAAGCAATACGAAGAAGTGCTCGAATGCGACTTCTCGTTCGCGGTGCCCAACCTCGCCCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGCCGCGGTGTTCCGGACCATTCCGTCGAAGGTCCTGTCGCTCGA
AGAACTCAACGCGCCGAAGATCTTCAAGGAAATCTCGAACCAGCCGCGCGGCATCGTGCTCGTGACCGGCCCGACCGGGT
CGGGCAAGTCGACGACGCTCGCGGCGATGATCGACCATGTCAACGACAACGAATACGGCCACATCCTGACGATCGAGGAC
CCGATCGAATTCGTGCATGAATCCAAGCGCTGCCTGATCAACCAGCGCGAAGTCGCGCGCGACACGCTGTCGTTCAACAA
CGCGCTGCGCTCGGCACTGCGTGAAGACCCGGACGTGATCCTCGTCGGCGAATTGCGCGACCTCGAAACCATCCGCCTCG
CGCTGACCGGCGCCGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
GTCGACGTCTTCCCCGCCGCCGAAAAGGAAATGGTCCGCTCGATGCTGTCCGAGTCGCTGCGTGCGGTCATCGCGCAGAC
GCTCTTGAAGACCAAGGACGGCCAGGGCCGCGTCGCCGCGCACGAGATCATGATCGGCACCCCCGCGATCCGCAACCTGA
TCCGCGAGAACAAGATCGCACAGATGTACTCCGCGATCCAGACCGGTCAGAACGTCGGCATGCAGACGCTGGACCAGAAC
CTCGCGGACCTCGTCCGGCGCAATATCGTCTCCGCCGGCGAAGCCAAGCAGAAGGCGCAGAACAAGGACAACTTCGCCTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H0PT91

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.101

99.711

0.769
  pilT Acinetobacter baumannii D1279779

77.101

99.711

0.769
  pilT Acinetobacter baumannii strain A118

77.101

99.711

0.769
  pilT Pseudomonas aeruginosa PAK

76.232

99.711

0.76
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.711

0.757
  pilT Acinetobacter baylyi ADP1

74.783

99.711

0.746
  pilT Legionella pneumophila strain Lp02

74.203

99.711

0.74
  pilT Legionella pneumophila strain ERS1305867

74.203

99.711

0.74
  pilT Neisseria meningitidis 8013

71.387

100

0.714
  pilT Neisseria gonorrhoeae MS11

71.098

100

0.711
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.412

98.266

0.682
  pilT Vibrio cholerae strain A1552

69.412

98.266

0.682
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.601

100

0.526
  pilU Acinetobacter baylyi ADP1

41.667

100

0.419
  pilU Vibrio cholerae strain A1552

40.58

99.711

0.405
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.711

0.402

Multiple sequence alignment